TissueLyser Adapter Sets

在TissueLyser II上同时高效破碎多达48或192个样本

S_1293_2_LS_QF_TissueLyser_Vortex_Adapter_Set_2x24

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TissueLyser Adapter Set 2 x 24

Cat. No. / ID:  69982

Two sets of adapter plates and two racks for use with 2 mL microcentrifuge tubes on the TissueLyser III & II
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Accessories
Tube Adapter Sets
Plate Adapter Sets
For
2 mL tubes
50 mL tubes
TissueLyser Adapter Sets 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。

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✓ Knowledgeable and professional Product & Technical Support

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Product Details

TissueLyser Adapter Sets专用于在QIAGEN TissueLyser II上同时高效破碎48或192个样本。TissueLyser Adapter Set 2 x 24包含一对TL Reaction-Tube Holders和24-Tube。每个支架可放置多达24个2 ml的微型离心管,建议使用Safe-Lock管(如Eppendorf 2 ml Safe-Lock微型管)。TissueLyser Adapter Set 2 x 96配合QIAGEN Collection Microtubes(置于架子上)和Collection Microtube Caps使用。Collection Microtubes容积为1.2 ml 。

Resources

补充实验方案 (3)
Up to 1 x 109 bacteria are disrupted and homogenized by bead-milling in a guanidine-thiocyanate-containing lysis buffer. After addition of ethanol, the sample is loaded onto an RNeasy Mini spin column. Total RNA binds to the RNeasy silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNase-free water.
试剂盒操作手册 (2)
For high-throughput disruption of biological samples
For efficient disruption of up to 48 or 192 samples in parallel on the TissueLyser
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks (2)
For high-throughput disruption of biological samples
Accessories for the simultaneous disruption of samples in tubes and 96-well plates on the TissueLyser III & II
Supplementary Protocols (3)
Up to 1 x 109 bacteria are disrupted and homogenized by bead-milling in a guanidine-thiocyanate-containing lysis buffer. After addition of ethanol, the sample is loaded onto an RNeasy Mini spin column. Total RNA binds to the RNeasy silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNase-free water.

FAQ

Can I precool the TissueLyser 2 x 24 Adapter Set or the 2 x 96 Adapter Set using liquid nitrogen?

We do not recommend using the adapters of the TissueLyser II with liquid nitrogen. Instead, the adapters can be precooled at –80°C, either in the freezer or on dry ice.

FAQ ID -9173
What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?

TissueLyser Adapter Set 2 x 96 should be used with Collection Microtubes (racked) and Collection Microtube Caps (cat. nos. 19560 and 19566 respectively).

FAQ ID -3086
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?

Yes, we have the following protocols:

  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
  • Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).
FAQ ID -1550
What is the maximum amount of tissue that can be disrupted using the 2 x 24 TissueLyser Adapter Set (cat. no. 69982)?

100 mg tissue per sample tube is the maximum amount we recommend when using the 2 x 24 Adapter Set. Larger amounts of tissue would require considered optimization, depending on the tissue type and condition.

When using 100 mg tissue, for best homogenization results, we recommend cutting the sample into 2 pieces of approximately 50 mg each prior to disruption and performing disruption twice, each time at 25 Hz for 5 min.

FAQ ID -9178