GeneRead Library Quant System

用于qPCR定量检测NGS文库

S_5055_NGS_QIAseqLibraryQuan_s
Configure at GeneGlobe
Find or custom design the right target-specific assays and panels to research your biological targets of interest.
GeneRead Library Quant Array

Cat. No. / ID:  333304

PCR arrays with accessory components for sample library quantification prior to next-generation sequencing
Copy order details
Configure at GeneGlobe To see pricing
Product TypeKit
GeneRead Library Quant Array
QIAseq Library Quant Assay Kit
GeneRead Library Quant System 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
Configure at GeneGlobe
Find or custom design the right target-specific assays and panels to research your biological targets of interest.

Features

  • 以芯片规格提供预分装的即用型、梯度稀释DNA标准品
  • 自动计算文库浓度
  • 实验方案简便、灵敏度高、动态范围宽
  • 与Illumina和Ion Torrent/Proton NGS平台兼容
  • 与多数qPCR仪兼容

Product Details

GeneRead Library Quant System为NGS文库制备提供简便的解决方案,确保NGS实验获得可靠结果。GeneRead Library Quant System采用real-time PCR技术,特异性定量检测两端有测序接头的DNA分子。

Performance

使用两种浓度的NGS文库,将GeneRead Library Quant System的灵敏度和动态范围与Agilent's High Sensitivity DNA Kit进行对比。第一个文库使用这两种试剂盒皆可进行定量检测,但浓度较低的第二个文库低于Agilent's 2100 BioAnalyzer的最低检测阈限。相反,GeneRead Library Quant Array能够对这两种不同浓度的文库进行成功的定量检测(参见 GeneRead Library Quant System enables quantification of libraries with concentrations below the detection limit of conventional methods)。
See figures

Principle

准确定量文库对于NGS平台的高效使用十分重要。但是,当前的分光广度法只能检测总核酸浓度。GeneRead Library Quant System则采用real-time PCR技术,仅定量检测两端有接头的DNA分子。只有两端有接头的DNA分子才能在乳液PCR(Ion Torrent)或桥式PCR(Illumina)中得到扩增,因此此方法能够准确定量检测可扩增的文库DNA。real-time PCR灵敏性高,能够定量检测低浓度文库,甚至是浓度低于传统分光光度法的检测阈限。

GeneRead Library Quant System提供管装和芯片规格,经过优化,适合配合GeneRead qPCR SYBR Green Mastermixes使用,具有出色的灵敏性和宽泛的动态范围。两种规格都提供适合Illumina或Ion Torrent平台的DNA标准品。芯片规格提供分装的5个浓度的10倍梯度稀释标准品,混合有PCR引物,一式三份。确保您的样本文库在检测范围之中(参见 Principle of GeneRead Library Quant System)。

See figures

Procedure

芯片工作流程(参见 GeneRead Library Quant Array workflow)使用两个10倍稀释的样本文库,确保其浓度在梯度稀释的DNA标准品检测范围内。将样本与Generead qPCR SYBR Green Mastermix混合,等分为三份,加入芯片中。进行PCR,将原始CT数据导出至提供的数据分析Excel文件中,进行文库浓度的自动计算(Illumina)或模板稀释因子计算(Ion Torrent/Proton)。

管装标准品的工作流程与芯片的工作流程相似,仅有少数不同。先制备5个10倍梯度稀释的DNA标准品,以及2个10倍梯度稀释的样本文库。这样可确保文库的浓度在标准品稀释液范围之内。下一步,将稀释的DNA标准品和样本文库与提供能够的PCR试剂和相应的GeneRead qPCR SYBR Green Mastermix混合。进行PCR,将原始CT数据导出至提供的数据分析Excel文件中,进行文库浓度的自动计算(Illumina)或模板稀释因子计算(Ion Torrent/Proton)。

See figures

Applications

GeneRead Library Quant Array和GeneRead Library Quant Kit能够在使用Illumina或Ion Torrent/Proton NGS平台测序前,快速、准确的检测样本文库浓度。

Supporting data and figures

Resources

分析软件 (8)
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 96-well plates for Ion Torrent libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for Rotor-Disc 100 rings for Ion Torrent libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) for 72-well Rotor rings for Illumina libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 384-well plates for Illumina libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 96-well plates for Illumina libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) for 72-well Rotor rings for Ion Torrent libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 384-well plates for Ion Torrent libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for Rotor-Disc 100 rings for Illumina libraries
产品介绍与指南 (2)
Accelerate your NGS performance through Sample to Insight solutions
Introducing QIAseq
PDF (450KB)
Accelerate your NGS performance through Sample to Insight solutions
安全数据表 (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.
试剂盒操作手册 (1)
Certificates of Analysis (1)
Brochures & Guides (2)
Introducing QIAseq
PDF (450KB)
Accelerate your NGS performance through Sample to Insight solutions
Accelerate your NGS performance through Sample to Insight solutions
Analysis Software (8)
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 384-well plates for Illumina libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 384-well plates for Ion Torrent libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 96-well plates for Illumina libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for 96-well plates for Ion Torrent libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for Rotor-Disc 100 rings for Illumina libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) or GeneRead Library Quant Array for Rotor-Disc 100 rings for Ion Torrent libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) for 72-well Rotor rings for Illumina libraries
New version – Excel-based data analysis template for use with GeneRead Library Quant Kit (assay format) for 72-well Rotor rings for Ion Torrent libraries
Kit Handbooks (1)