Investigator Casework GO! 试剂盒

用于直接扩增案件样本

S_1084_5_GEN_V2

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Investigator Casework GO! 试剂盒

Cat. No. / ID:  386546

Casework GO! 裂解缓冲液、蛋白酶 K 溶液和无核酸酶水
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KitAccessories
Casework GO! Kit
DTT
Investigator Casework GO! 试剂盒旨在用于法医鉴定、人类身份识别、亲子鉴定等领域的分子生物学应用场景。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 裂解法医案件样本以供随后在直接扩增工作流程中使用
  • 最大限度地提高从各种痕量样本中回收 DNA 的概率
  • 实现 Y 染色体筛选和选择证据样本进行 STR 测试

Product Details

Investigator Casework GO! 试剂盒可用于对包括性侵犯拭子、织物碎片、纸张、烟头、口香糖和其他样品类型在内的各种基质上的法医鉴定样本进行裂解。生成的裂解物与所有 Investigator Quantiplex 和 Investigator STR QS 系统都兼容。Investigator Casework GO! 试剂盒符合 ISO 18385 要求。

Principle

Investigator Casework GO! 试剂盒用于在直接扩增工作流程中对法医鉴定样本进行预处理。该试剂盒可裂解样本且无需 DNA 纯化,并且裂解物与几种下游选项兼容,包括 Investigator Quantiplex Pro 和 Quantiplex Pro RGQ 或 Investigator 24plex QS、26plex QS、ESSplex SE QS 或 IDplex Plus Kit。

Investigator Casework GO! 试剂盒可用于直接扩增案件样本,从而实现加速案件分析工作流程的目的。它不需要进一步纯化便可快速处理来自案件样本的拭子,包括性侵犯拭子的切片或其他材料。裂解物可直接用于所有 Investigator Quantiplex 检测的定量,或用于使用所有 Investigator STR 检测的 DNA 分型(除非定量数据表明存在可能的 PCR 抑制剂)。

在性侵犯筛查分析中,Investigator Casework GO! 工作流程可以通过男性定量和常染色体/Y 染色体比率来帮助做出样本处理决策。

Procedure

加 100-400 µl 的 Casework GO! Master Mix 添加到试管内的样本中,并在 60°C 下孵育 25 分钟,然后在 80°C 下再孵育 5 分钟。使用 Quantiplex Pro Kit 定量 2-5 μl 的该裂解物,并将最多 15 μl 裂解物用于使用 Investigator STR QS Kit 进行扩增。

Applications

案件样本

许多大规模犯罪样本(例如,来自入室盗窃或车辆相关犯罪的样本)未经过检测,因为没有资金或时间可用于样本制备。使用 Investigator Casework GO! 试剂盒,可以免除样本制备步骤,使 DNA 图谱的生成更快速、成本更低,并且可以提高对更大数量的大规模犯罪样本进行 DNA 检测的可行性。使用 Investigator Casework GO! Kit 时可以获得有价值的工作流程信息,如男性/总人类 DNA 的存在/不存在、样本降解或者是存在潜在抑制剂试剂盒和 Quantiplex Pro 试剂盒或 Investigator STR QS 试剂盒搭配使用

性侵犯证据/Y 染色体筛查

许多性侵犯案件都被封存了数月甚至数年,因为恢复适用于 PCR 的 DNA 的工作量和复杂性过高。按照最新的 SWGDAM 建议采用“直达 DNA”方法,可最大限度提高只对值得充分处理的样本做进一步处理的几率。Investigator Casework GO! Quantiplex Pro 工作流程确定男性/女性 DNA 比率并预测 PCR 抑制以及样本降解。因此,它可以提供快速的 Y 染色体筛查和质量评估,以便选择证据样本做进一步的 STR 检测。

Resources

快速启动实验方案 (1)
产品介绍与指南 (1)
技术资讯 (2)
Quality initiatives for human identity testing and forensics 
其他资源 (1)
应用说明 (2)
Application Note Investigator Casework GO!  Kit
Application Note Skin flake isolation with the Investigator Casework GO! Kit
Certificates of Analysis (1)
Application Notes (2)
Application Note Investigator Casework GO!  Kit
Application Note Skin flake isolation with the Investigator Casework GO! Kit
Technical Information (2)
Quality initiatives for human identity testing and forensics 
Quick-Start Protocols (1)
Supplementary Protocols (1)
Additional Resources (1)

FAQ

Why is DTT used only for semen samples?
The DTT breaks down the protein disulfide bridges that make up the sperm nuclear membranes. Thus, DTT is always required for semen lysis.

It may also be used for all samples to keep the process simple, but it is not required to lyse blood, epithelial cells, etc. 
FAQ ID - 143761
Is it possible to use Lyse&Spin baskets?
It is possible, but modifications to the heat incubation is required because the temperature transfer to the sample might decrease due to the two plastic walls of the vessel.

This could reduce the yield and ProtK inactivation efficiency.

Note: ProtK inactivation must be done after sample collection and removal of the basket.
FAQ ID - 143762
How long are sample lysates stable in the fridge/ freezer?
Store samples at 2–8°C for storage up to 2 years, or at −30°C to −15°C for storage periods >2 years.

FAQ ID - 143765
How critical is the time of the temperature change from 60°C to 80°C?
There is no adverse effect for samples remaining in the heater during temperature increase. Nor does it make a difference if samples are kept on the bench until the heater has reached its temperature.

If only one heater shaker is available, the protocol will take a few minutes longer than the usual processing time.
FAQ ID - 143768
If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.)
Different concentration devices are available on the market, but are not tested with the kit. Testing needs to be done first by customer.

Another helpful modification is the starting lysis volume, which can be reduced for most of the swab type to 200 µl. This helps to have higher sample concentrations.




FAQ ID - 143766
What if only one heater shaker is available?
The usage of only one heater shaker does not adversely affect the outcome.

The process will just last a few minutes longer than the usual processing time.
FAQ ID - 143764
Can the lysis volume be reduced for higher sensitivity?
The ideal lysis volume needs to be determined for each collection device.

For cotton swabs (e.g. Sarstedt Forensic swabs), 200 µl are usually sufficient.

Cuttings can be lysed with 100 µl Casework Go! Buffer depending on the size.
FAQ ID - 143763
Why is there no male DNA obtained from sexual assault samples?
DTT is not stable. The 1M solution should be stored frozen in small aliquots (50 µl) and disposed after thawing.

Repeat with freshly made DTT solution.
FAQ ID - 143767