Supplemental Microbial qPCR Mastermix (ROX)

用于real-time PCR

S_1084_5_GEN_V2

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Supplemental Microbial qPCR Mastermix (ROX)

Cat. No. / ID:  330530

2 tubes of 1.35 ml each
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Supplemental Microbial qPCR Mastermix (ROX) 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 使用5'端水解探针法检测的多功能PCR预混液
  • 产物特异性高,引物二聚体少
  • 可在使用ROX染料作为参比染料的PCR仪上进行微生物分析

Product Details

Supplemental Microbial qPCR Mastermix (ROX)采用5'端水解探针法检测,十分适用于real-time PCR,适合用于使用ROX染料作为参比染料的PCR仪。如自动移液体系在Microbial DNA qPCR Arrays and Assay Kits提供的预混液之外,需要更多预混液,则可使用此产品。Supplemental Microbial qPCR Mastermix (ROX)含有real-time PCR缓冲液、高性能的HotStart DNA Taq聚合酶、核苷酸、以及用于优化仪器光学性能的ROX染料。HotStart酶经化学修饰和严格的质检,采用5'端水解探针技术,避免扩增生产引物二聚体和非特异性产物,可获得准确结果。

Principle

Supplemental Microbial qPCR Mastermix (ROX)含有real-time PCR缓冲液、高性能HotStart DNA Taq聚合酶、核苷酸、以及用于优化PCR仪光学性能的ROX染料。HotStart酶经过化学修饰和严格的质检,采用5'端水解探针技术,避免生产引物二聚体和非特异性产物,可获得准确的扩增结果。

Applications

某些自动化移液体系需要除Microbial DNA qPCR Arrays and Assay Kits中预混液以外的更多预混液,这时可使用Supplemental Microbial qPCR Mastermix (ROX)。

Resources

安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
技术资讯 (1)
试剂盒操作手册 (1)
For real-time PCR-based profiling/detection of microbial species, antibiotic resistance genes or virulence factor genes
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks (1)
For real-time PCR-based profiling/detection of microbial species, antibiotic resistance genes or virulence factor genes
Technical Information (1)

FAQ

What are the storage conditions for the Microbial DNA qPCR products?
Store Microbial DNA qPCR Array plates at room temperature.
Store Microbial DNA qPCR Assays, Microbial DNA qPCR Assay Kits, and Microbial DNA qPCR Multi-Assay Kits at –20°C.
Store Microbial qPCR Mastermix at –20°C.
Store Microbial DNA Positive Control at –20°C.
Store Microbial DNA-Free Water at –20°C.

FAQ ID — 3394
Which Microbial qPCR Mastermix should I use?
The Microbial qPCR Mastermix (ROX) is suitable for use with the following real-time cyclers: all Applied Biosystems and Agilent (formerly Stratagene); BioRad Opticon, Opticon 2, and Chromo 4; Roche LightCycler 480; and Eppendorf Mastercycler ep realplex 2, 2S, 4, and 4S.

The Microbial qPCR Mastermix (Fluorescein) is suitable for use with the following real-time cyclers: BioRad iCycler, MyiQ, and iQ5.

FAQ ID — 3395