GeneRead DNA QuantiMIZE Kits

用于在NGS前,定量、定性检测可扩增DNA

S_5056_NGS_QIAseqDNA_s
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QIAseq DNA QuantiMIZE Assay Kit

Cat. No. / ID:  333414

Assay 100 and Assay 200 for 400 x 25 µl reactions (400 µl) each; QuantiMIZE Control gDNA (50 µl); RNase- and DNase-free water (1 ml x 4); GeneRead qPCR SYBR Green Mastermix (1.35 ml x 4)
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GeneRead DNA QuantiMIZE Kits 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。
Configure at GeneGlobe
Find or custom design the right target-specific assays and panels to research your biological targets of interest.

Features

  • 在Array或单独的反应管中实施定性或定量类PCR分析
  • 对PCR扩增后的DNA进行定性和定量
  • 指导靶向富集条件的优化
  • 无需进行梯度稀释

Product Details

GeneRead DNA QuantiMIZE System是基于qPCR方法的检测系统,适于在NGS分析之前对即将进行靶向富集扩增的DNA进行定量与定性分析。本系统专为探索型、转化型和临床型等不同类型的研究人员而研发。这一类人员往往面对的是分离自包括FFPE等不同生物样本的DNA,需要更为经济、高效的检测手段来确定靶向富集DNA的最优条件。不同于其他技术手段,此系统不仅能够对可扩增的DNA进行定量,还能同时优化靶向富集条件,从而提升NGS检测的成功率。

Performance

通过GeneRead DNA QuantiMIZE System对可扩增的DNA进行定量和定性操作,能够成功从低质量的FFPE样本中获得有效样本进入后续的NGS分析。使用GeneRead DNA QuantiMIZE System可获得高质量的文库,进而在MiSeq设备上成功实施测序(参见 Successful quantification and qualification of DNA by the GeneRead DNA QuantiMIZE system)。
See figures

Principle

GeneRead DNA QuantiMIZE System应用了两类qPCR分析,能够对20个基因组位点进行查询以确定生物样本中可扩增DNA的数量。这两类分析——即Assay 100和Assay 200——能够分别生成100和200 bp的扩增子。QuantiMIZE Kit使用Assay 100和Assay 200分析中获得的CT值来同时为对照(Control)样本和检测(Case)样本的DNA进行定量和定性(参见 Principle of the GeneRead DNA QuantiMIZE System)。
See figures

Procedure

将生物样本中抽提出的基因组DNA与所提供的预混液进行混合。如果使用了芯片试剂盒,则将混合之后的样品平均加样至芯片当中;如果使用了分析试剂盒,则在加样至芯片之前将其与Assay 100和Assay 200进行混合。执行PCR反应并将原始的CT值导出至网络数据分析模块当中,后者将对DNA进行定量和定性,并提供所需的DNA数量和PCR循环数方面的指导意见,以帮助优化靶向富集条件。

Applications

GeneRead DNA QuantiMIZE Kit适合于针对任何种类DNA样本进行定量和定性操作。其尤为适于FFPE等低质量DNA样本。不仅如此,为了成功地实施靶向富集操作,此试剂盒还能够针对任何种类的DNA样本中的基因组区域,进行条件优化方面的必要工作;其所兼容的DNA样本囊括了FFPE等低质量DNA样本和细针吸取液等低含量DNA样本。

Supporting data and figures

Resources

产品介绍与指南 (1)

Next-generation sequencing using QIAGEN’s QIAseq – a powerful tool for human identification


安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
分析软件 (4)
Excel-based data analysis template for use with QuantiMIZE assay kits for 72-well plates
Excel-based data analysis template for use with QuantiMIZE array and assay kits for Rotor-Disc 100 rings
Excel-based data analysis template for use with QuantiMIZE array and assay kits for 96-well plates
Excel-based data analysis template for use with QuantiMIZE array and assay kits for 384-well plates
试剂盒操作手册 (1)
New version – For advanced qualification and quantification of amplifiable genomic DNA for targeted enrichment prior to next-generation sequencing (assay format)
Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (1)

Next-generation sequencing using QIAGEN’s QIAseq – a powerful tool for human identification


Analysis Software (4)
Excel-based data analysis template for use with QuantiMIZE array and assay kits for 384-well plates
Excel-based data analysis template for use with QuantiMIZE assay kits for 72-well plates
Excel-based data analysis template for use with QuantiMIZE array and assay kits for Rotor-Disc 100 rings
Excel-based data analysis template for use with QuantiMIZE array and assay kits for 96-well plates
Kit Handbooks (1)
New version – For advanced qualification and quantification of amplifiable genomic DNA for targeted enrichment prior to next-generation sequencing (assay format)

FAQ

How do I obtain the concentration of my gDNA from the data analysis using the GeneRead DNA QuantiMIZE?

The data analysis template provides the number of cycles and volume of gDNA to use with our GeneRead workflow but no absolute DNA concentration.

FAQ ID - 3530
Can I use the QuantiMIZE system for determination of gDNA quality for non-multiplex PCR enrichment methods (ex. Hybridization) (cat. No. NGQA-002, NGQC-100Y)?

No. This system is best for determining gDNA quality for multiplex PCR enrichment methods only.

FAQ ID - 3531