RNase A
用于在DNA制备时进行RNase消化
Get 5% off the enzyme of your choice.
Use the code ENZ2526 at checkout. Offer valid from December 1, 2025, to March 31, 2026. T&Cs* apply.
RNase A
目录编号 / ID. 19101
2.5 ml (100 mg/ml; 7000 units/ml, solution)
*Offer valid on orders placed from December 1, 2025, to March 31, 2026, or while stocks last. Offer void where prohibited and cannot be combined with any other promotion. Offer is only valid in the following countries: Australia, Austria, Belgium, Canada, Denmark, Finland, France, Germany, Italy, Luxembourg, Netherlands, New Zealand, Norway, Poland, South Africa, Sweden, Switzerland, United Kingdom, United States of America. Freight and delivery costs are not included. It is the customer's responsibility to ensure that acceptance of this offer will not violate any internal policies of the customer's organization and applicable laws and regulations.
RNase A 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
Get 5% off the enzyme of your choice.
Use the code ENZ2526 at checkout. Offer valid from December 1, 2025, to March 31, 2026. T&Cs* apply.
特点
- Selectively cleaves single-stranded RNA
- Active under a wide range of reaction conditions
- DNase-free and quality-controlled
- Does not need to be boiled before use
产品详情
QIAGEN Ribonuclease A（RNase A）不含核酸内切酶，并经过质量控制，适用于在质粒纯化实验中消化RNA。这一即用型溶液与QIAGEN质粒DNA纯化试剂盒中的RNase具有相同规格参数。使用时需要将溶液等分、在Buffer P1中稀释至合适浓度。
绩效
QIAGEN Ribonuclease A is DNase-free and quality-controlled for use in plasmid purification procedures for the digestion of RNA.
RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA. RNase A is also used to remove contaminating RNA from DNA samples after extraction or PCR, especially if downstream applications (e.g., cloning or sequencing) require RNA-free DNA.
RNase A is very active under a wide range of reaction conditions and is difficult to inactivate.
QIAGEN RNase A does not need to be boiled before use.
| Feature | RNase A |
|---|---|
| Quantity | 2.5 mL |
| Concentration | 100 mg/; 7000 units/ml, solution |
| Unit definition | One Kunitz unit is the amount of enzyme causing the hydrolysis of RNA at a rate such that the velocity constant (k) equals unity at 25°C and pH 5.0 |
程序
Before use in plasmid and DNA purification, centrifuge QIAGEN RNase A briefly, and then add into Buffer P1 to obtain a final concentration of 100 μg/mL. Store Buffer P1 at 2–8°C after addition of RNase A. For detailed instructions on RNase A usage during plasmid and DNA purification, please refer to the corresponding kit handbook.
应用
- Plasmid and genomic DNA preparation
- Removal of RNA contamination from recombinant protein preparations
- Cleanup of RNA from enzymatic reactions
资源
安全数据表 (1)
产品介绍与指南 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
常见问答
3314-What is the concentration of RNase A sold separately?