Puregene Accessories

从各种类型的样本中纯化可存档的DNA

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Proteinase K (5 ml)

Cat. No. / ID:  158146

5 ml Puregene Proteinase K
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EnzymeSolution
Enzyme
Solution
Type
Proteinase K
RNase A
Volume
5 ml
650 µl
The implementation of an in-house manufacturing solution has lead to product name and catalogue number changes in the Puregene portfolio . This change will not reflect on the quality and performance of the products.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 高品质DNA用于长期储存
  • 高重复性的DNA纯化
  • 方便、可扩展的纯化操作

Product Details

Puregene Accessories配合Gentra Puregene Kits纯化高分子量(100–200 kb)DNA,适合用于存档。可扩展的纯化操作,能温和的去除大体积样本中的污染物和抑制剂,纯化获得的大体积样本适合长期保存。

Performance

 

Catalog number changes in the Puregene line.
NEW product name NEW cat.no. Old product name Old cat. no.
158103 Puregene RBC Lysis Solution (450 ml) 158902 RBC Lysis Solution(450 ml)
158106 Puregene RBC Lysis Solution (1000 ml) 158904 RBC Lysis Solution (1000 ml)
158113 Puregene Cell Lysis Solution (125 ml) 158906 Cell Lysis Solution (125 ml)
158116 Puregene Cell Lysis Solution (1000 ml) 158908 Cell Lysis Solution (1000 ml)
158123 Puregene Protein Precipitation Sol. (50 ml) 158910 Protein Precipitation Solution (50 ml)
158126 Puregene Protein Precipitation Solution (350 ml) 158912 Protein Precipitation Solution (350 ml)
158133 Puregene DNA Hydration Solution (100 ml) 158914 DNA Hydration Solution (100 ml)
158136 Puregene DNA Hydration Solution (500 ml) 158916 DNA Hydration Solution (500 ml)
158143 Proteinase K (650 µl) 158918 Puregene Proteinase K (650 µl)
158146 Proteinase K (5 ml) 158920 Puregene Proteinase K (5 ml)
158153 RNase A Solution (650 µl) 158922 RNase A Solution (650 µl)
158156 RNase A Solution (5 ml) 158924 RNase A Solution (5 ml)
158183 Glycogen Solution (500 µl) 158930 Glycogen Solution (500 µl)
158186 Glycogen Solution (5 ml) 949002 Autopure Glycogen Solution (5 ml)

Principle

应用Gentra Puregene Kits,细胞用阴离子洗涤剂裂解,其中加入DNA稳定剂。DNA稳定剂限制胞内及其他环境中的DNases的活性。使用RNA降解酶去除RNA。蛋白质等其他污染物经盐析沉淀法去除。最终,通过乙醇沉淀回收基因组DNA,水合溶液(1 mM EDTA、10 mM Tris·Cl pH 7.5)溶解DNA。一般情况下,纯化的DNA的A260/A280值在1.7–1.9之间,大小可达200 kb。纯化的DNA可贮存于2–8°C、–20°C或–80°C,确保安全。

Procedure

可存档的DNA  

DNA的纯度对下游应用结果的精确性至关重要,诸如PCR等敏感的下游应用要求使用最高品质、且分子量尽可能大的DNA,以确保可靠的结果。Gentra Puregene Kits去除污染物和酶抑制剂,确保获得高度稳定的适合存档的DNA。纯化获得高品质的DNA,分子大小为100–200 kb。

Applications

应用Puregene Accessories和Gentra Puregene Kits纯化的DNA高度稳定,适用于各种下游应用,包括:

  • DNA存档
  • PCR
  • SNP分析
  • Southern印迹

Resources

Safety Data Sheets (1)
Certificates of Analysis (1)

Publications

Methylation variation at IGF2 differentially methylated regions and maternal folic acid use before and during pregnancy.
Hoyo C; Murtha AP; Schildkraut JM; Jirtle RL; Demark-Wahnefried W; Forman MR; Iversen ES; Kurtzberg J; Overcash F; Huang Z; Murphy SK;
Epigenetics; 2011; 6 (7):928-36 2011 Jul 1 PMID:21636975

FAQ

What is the composition of RBC Lysis Solution?
The exact composition of RBC Lysis Solution is confidential. RBC Lysis Solution is a component of Gentra Puregene Blood Kits. RBC Lysis Solution selectively lyses human red blood cells leaving white blood cells intact. You can order RBC Lysis Solutin separately.
FAQ ID -2808
What is the composition of DNA Hydration Solution in Gentra Puregene Kits?

The composition of DNA Hydration Solution is as follows:

  • 10 mM Tris
  • 1 mM EDTA
  • pH 7–8

DNA Hydration Solution is included in QIAGEN’s Gentra Puregene Kits for DNA purification and can also be bought separately from QIAGEN.

FAQ ID -2813
What is the composition of Cell Suspension Solution?
The detailed composition of Cell Suspension Solution is confidential. Cell Suspension Solution is a component of the Gentra Puregene Yeast/Bacteria Kit. Essentially, it is an isotonic solution that prevents spheroblasts from bursting. Cell Suspension Solution can be ordered separately.
FAQ ID -2811
What is the composition of Protein Precipitation Solution?
The exact composition of Protein Precipitation Solution is confidential. Protein Precipitation Solution is a component of Gentra Puregene Kits for DNA purification. Essentially, this is a high-salt buffer that lowers the solubility of proteins. Protein Precipitation Solution can be ordered separately.
FAQ ID -2810
What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?
The exact composition of Glycogen Solution is confidential. The Glycogen concentration is 20mg/mL. Glycogen Solution is a component of Gentra Puregene Kits for DNA purification. During isopropanol precipitation, Glycogen Solution acts as a nucleic acid carrier and helps to efficiently precipitate small amounts of DNA. In addition, it facilitates visualization of the DNA pellet. Glycogen Solution can be purchased separately.

 

Note: Glycogen Solution does not work in combination with QIAGEN’s silica membrane technologies, e.g., RNeasy, DNeasy, QIAamp, etc. With these technologies, poly-A carrier RNA is used when dealing with low amounts of nucleic acids.

 

 

FAQ ID -2812
What is the composition of Cell Lysis Solution?
The exact composition of Cell Lysis Solution is confidential. Cell Lysis Solution is a component of Gentra Puregene Kits for DNA purification. It functions by enabling cell lysis. Cell Lysis Solution can be purchased separately.
FAQ ID -2809
What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?
The Puregene RNase A and Autopure RNase A solutions both have a concentration of 4 mg/ml.

 

FAQ ID -3083
What do you recommend for the cleanup of genomic DNA (gDNA)?

Using QIAGEN Genomic-tips

Protocol for DNA cleanup using Genomic-tips can be found in the 'Special Applications' section of the QIAGEN Genomic DNA Handbook.

 

Using QIAamp DNA Micro kit

Up to 10ug of gDNA can be cleaned using the cleanup protocol in the QIAamp DNA Micro Handbook.

 

Using Gentra Puregene Reagents

Gentra Puregene Handbook has protocols for removal of proteins and RNA from purified gDNA in Appendix C and Appendix D respectively.

FAQ ID -618