PolyFect Transfection Reagent

快速简单的DNA 转染,用于标准细胞株

S_1084_5_GEN_V2

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PolyFect Transfection Reagent (1 ml)

Cat. No. / ID:  301105

For 25–65 transfections in 60 mm dishes or 50–100 transfections in 6-well plates.
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Quantity
1 ml
4 x 1ml
100 ml
PolyFect Transfection Reagent 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 优化的细胞特异性转染方案
  • 操作简便迅速
  • 细胞存活率高,毒性低

Product Details

PolyFect Transfection Reagent采用先进的活化树状多聚物,从多聚物中心向外辐射出带正电荷的氨基基团与DNA 分子结合,为下列细胞株特别优化:COS-7、NIH/3T3、HeLa、293、CHO。

Performance

PolyFect Transfection Reagent保持高效转染率(参见" High transfection efficiencies in CHO and 293 cells"和" PolyFect Reagent with HeLa cells")。与其他试剂不同的是,PolyFect Transfection Reagent能够在血清存在的情况下进行转染而不降低转染效率。

See figures

Principle

PolyFect Transfection Reagent是一种特殊活化的树状聚合物。它由从中心发散出分支的球形大分子构成(参见" Activated dendrimer")。分支终止于带电的氨基,可与带相反电荷的磷酸基相互作用。PolyFect Reagent将DNA聚集成紧密结构(参见 " PolyFect–DNA interaction"),附于细胞表面通过非特异性内吞作用进入细胞。该试剂调节细胞核内pH,抑制核内核酸,从而保证了PolyFect–DNA复合物的稳定性。

See figures

Procedure

PolyFect Transfection Reagent是即用型试剂。转染流程(参见" PolyFect transfection procedure")简便迅速,只需要将试剂加入到DNA溶液中混合,反应5–10分钟,加入培养基(可以含血清和抗体),将PolyFect–DNA复合物移到细胞上。培养细胞用于基因表达。无需转染后移除复合物或更换及增加培养基,操作简便快速。

高通量转染

使用PolyFect Transfection Reagent进行转染只需少量手动操作且无需移除转染复合物,特别适用于高通量筛选。PolyFect Reagent在高通量转染中转染效率高,可重复性好,细胞毒性低,适用于批量转染。关于在96孔板中对COS-7、NIH/3T3、HeLa、HeLa-S3、293和CHO细胞进行转染的实验方案,请咨询QIAGEN技术支持。

See figures

Applications

PolyFect Transfection Reagent特别适用于标准细胞系高效转染,主要用于:

  • 基因表达与功能研究
  • 药物研究与开发

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsRNAi studies, gene expression studies...
Transfection typeTransient transfection, stable transfection, plasmid co-transfection
ControlsNot included
FeaturesOptimized protocols for COS-7, NIH/3T3, HeLa 293, and CHO cells. Transfection in the presence of serum
TechnologyActivated dendrimers
Number of possible transfections25–10,000 transfections
Nucleic acidDNA
Cell typeCOS-7, NIH/3T3, HeLa, 293, CHO cells

Resources

转染实验方案 (2)
Search for transfection data by nucleic acid, cell line, and transfection reagent. Our database contains data from researchers like yourself who have shared their experimental results with us.
Transfection protocols for specific cell types and plate formats that save you the time and effort of adapting existing protocols to fit your requirements. Simply select the cell type, nucleic acid, and culture format to receive a QIAGEN transfection protocol to print out or download in convenient PDF format.
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
补充实验方案 (13)
The following protocol is optimized for transient transfection of COS-7 cells in 96-well plates.
The following protocol is optimized for transient transfection of CHO cells in 96-well plates.
The following protocol is optimized for transient transfection of 293 cells in 96-well plates.
The following protocol is optimized for transient transfection of NIH/3T3 cells in 96-well plates.
The following protocol is optimized for transient transfection of HeLa-S3 cells in 96-well plates. Please note that for HeLa cells the optimal amounts of DNA and PolyFect® Reagent differ from the amounts given in the protocol below. Please call one of the QIAGEN Technical Service Departments or your local distributor for an optimized HeLa protocol.
The following protocol is optimized for transient transfection of HeLa-S3 cells in 60 mm dishes. Parameters for transfection using other culture formats are given in Table 1. Please note that this protocol is only for HeLa-S3 cells. Optimized protocols for HeLa cells are provided in the PolyFect® Transfection Reagent Handbook.
The following protocol is optimized for transient transfection of HeLa cells in 96-well plates. Please note that for HeLa-S3 cells the optimal amounts of DNA and PolyFect® Reagent differ from the amounts given in the protocol below. Please call one of the QIAGEN Technical Service Departments or your local distributor for an optimized HeLa-S3 protocol.
产品介绍与指南 (1)
Brochure detailing reagents for efficient and robust DNA and RNA transfection.
试剂盒操作手册 (1)
For optimized transfection of COS-7, NIH/3T3, HeLa, 293, and CHO cells
快速启动实验方案 (1)
用户开发的实验方案 (1)
This procedure has been developed by customers for the transient transfection of HEK 293 cells in 75 cm3 flasks using QIAGEN® PolyFect® Transfection Reagent. It has not been thoroughly tested and optimized by QIAGEN.
Certificates of Analysis (1)
Brochures & Guides (1)
Brochure detailing reagents for efficient and robust DNA and RNA transfection.
Kit Handbooks (1)
For optimized transfection of COS-7, NIH/3T3, HeLa, 293, and CHO cells
Transfection Protocols (2)
Search for transfection data by nucleic acid, cell line, and transfection reagent. Our database contains data from researchers like yourself who have shared their experimental results with us.
Transfection protocols for specific cell types and plate formats that save you the time and effort of adapting existing protocols to fit your requirements. Simply select the cell type, nucleic acid, and culture format to receive a QIAGEN transfection protocol to print out or download in convenient PDF format.
Supplementary Protocols (13)
The following protocol is optimized for transient transfection of HeLa-S3 cells in 60 mm dishes. Parameters for transfection using other culture formats are given in Table 1. Please note that this protocol is only for HeLa-S3 cells. Optimized protocols for HeLa cells are provided in the PolyFect® Transfection Reagent Handbook.
The following protocol is optimized for transient transfection of HeLa-S3 cells in 96-well plates. Please note that for HeLa cells the optimal amounts of DNA and PolyFect® Reagent differ from the amounts given in the protocol below. Please call one of the QIAGEN Technical Service Departments or your local distributor for an optimized HeLa protocol.
The following protocol is optimized for transient transfection of CHO cells in 96-well plates.
The following protocol is optimized for transient transfection of COS-7 cells in 96-well plates.
The following protocol is optimized for transient transfection of 293 cells in 96-well plates.
The following protocol is optimized for transient transfection of HeLa cells in 96-well plates. Please note that for HeLa-S3 cells the optimal amounts of DNA and PolyFect® Reagent differ from the amounts given in the protocol below. Please call one of the QIAGEN Technical Service Departments or your local distributor for an optimized HeLa-S3 protocol.
The following protocol is optimized for transient transfection of NIH/3T3 cells in 96-well plates.
Quick-Start Protocols (1)
User-Developed Protocols (1)
This procedure has been developed by customers for the transient transfection of HEK 293 cells in 75 cm3 flasks using QIAGEN® PolyFect® Transfection Reagent. It has not been thoroughly tested and optimized by QIAGEN.

FAQ

What are your recommendations for cotransfecting several plasmids?
The recommended amount of DNA being transfected should be split between the different plasmids. For example, if you normally transfect 5 µg of one plasmid, use 2.5 µg each for two plasmids (other proportions of the two plasmids can be used, as long as 5 µg total is maintained) as a starting point for optimization.
FAQ ID -124
Do you have transfection data for QIAGEN Transfection Reagents?

QIAGEN Transfection Reagents have been used successfully with many different cell types. For your convenience, we have organized data from researchers who have shared their experimental results with us online in our Transfection Cell Database. Simply type your cell line of interest into the 'Search' field on this page, and find transfection results achieved with various QIAGEN Transfection Reagents.  Please note that QIAGEN cannot verify data supplied from outside sources.

You can also submit your own transfection data and obtain a gift as appreciation.  In addition you can find on our TransFect Protocol Database transfection protocols for specific cell types and plate formats.

FAQ ID -158
How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent?

For the transfection of oligonucleotides and/or large vector constructs (>20 kb) using PolyFect Transfection Reagent, we recommend performing optimization trials to determine the optimal Transfection Reagent/DNA ratio. The amounts of DNA and Reagent provided in the PolyFect Transfection Reagent Handbook can be used as a guideline.

We suggest to start optimization using a range between half and twice the recommended amount of PolyFect Transfection Reagent while keeping the DNA amount constant, as well as using half and twice the recommended amount of DNA while keeping the amount of PolyFect Transfection Reagent constant. For increasing the transfection efficiency of adherent cells you can use the Attractene Transfection Reagent, or of primary cells and difficult-to-transfect cells using Effectene Transfection Reagent. "

FAQ ID -176
Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks?
FAQ ID -1014
Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect?

Yes, please follow the Supplementary Protocol 'Transient Transfecton of HeLa-S3 cells in 60 mm dishes using PolyFect Transfection Reagent' (TFP01).

FAQ ID -1012
Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent?
What is the recipe for 1x PBS solution?

The composition of 1x PBS solution is:

  • 137 mM NaCl
  • 2.7 mM KCl
  • 4.3 mM Na2HPO4
  • 1.47 mM KH2PO4

Adjust to a final pH of 7.4.

This solution is not supplied in any QIAGEN Kit, but is used in protocols for various QIAGEN transfection kits.

FAQ ID -1030
Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent?

 Yes, we have a Supplementary Protocol for 60 mm dishes and 96-well plates:

FAQ ID -997