FlexiGene DNA Kit

用于从单个试管内的全血、血沉棕黄层和培养细胞中可扩展地分离基因组 DNA

S_1624_RPA_QA_1070

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FlexiGene DNA Kit (250)

Cat. No. / ID:  51206

用于通过快速试剂盒制备隔离来自单个试管内全血、血沉棕黄层和培养细胞中的基因组 DNA
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FlexiGene DNA Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 操作简便,无样本混淆风险
  • 可从 0.1-20 ml 全血中进行可扩展纯化
  • 直接从全血、血沉棕黄层和培养细胞中纯化
  • 快速操作流程
  • 无有机萃取

Product Details

FlexiGene 系统利用简单的离心操作,从全血、血沉棕黄层和培养细胞中分离高纯度 DNA。FlexiGene DNA Kit 包含缓冲液和 QIAGEN Protease,可直接在单管中进行简单的纯化。有关使用 FlexiGene 试剂的自动化解决方案,请联系 Autogen, Inc.。

Performance

使用 FlexiGene 系统纯化的 DNA 大小可达 150 kb(见图“ 高品质基因组 DNA”)。它在扩增和南方印迹法实验中表现出色,也适用于归档(见图“ 高效的下游 PCR 分析”)。

从经离心处理的单管内的 0.1-20 ml(血液)、0.1-2 ml(血沉棕黄层)或 1-2 x 106 个细胞(培养细胞)中纯化 DNA。1 小时内可处理十二个样本。根据样本量的不同,补液量为 0.2-1 ml。

使用 FlexiGene 操作流程从不同类型样本中纯化出的 DNA 产量
样本体积DNA 产量 (µg)
血液*0.3 ml11-14
血液*2.0 ml75-90
血液*10.0 ml380-460
血沉棕黄层0.5 ml110-130
HeLa 细胞106 个细胞25-35
See figures

Principle

FlexiGene DNA 系统可从不同量的全血、血沉棕黄层和培养细胞中分离基因组 DNA。简单的纯化操作流程在单管内完成,从而降低成本并简化操作。纯化的 DNA 在敏感的下游应用中表现出色。

Procedure

第一步是在样本中加入裂解缓冲液(见流程图“ FlexiGene DNA 操作流程”)。通过离心将细胞核和线粒体沉淀,然后在含有 QIAGEN Protease 的变性缓冲液中重新悬浮。蛋白消化后,添加异丙醇沉淀 DNA,离心回收,用 70% 乙醇洗涤,然后干燥。DNA 在水化缓冲液中重新悬浮后,即可直接用于下游检测或在 –20ºC 下储存。从全血、血沉棕黄层和培养细胞中获得高产量的 DNA(见表“使用 FlexiGene 操作流程从不同类型样本中纯化出的 DNA 产量”)。
See figures

Applications

使用 FlexiGene DNA 试剂盒纯化的 DNA 在下游应用中表现出色,包括

  • PCR
  • 南方印迹法
  • 归档

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR 技术、限制性消化、南方印迹法、测序
Sample amount100 µl - 20 ml
Format可扩展
Time per run or per prep25 - 45 分钟(+ DNA 复水)
Main sample type全血、血沉棕黄层、培养细胞
Technology单管沉淀法
Processing手动
Yield35 µg/ml 全血
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein基因组 DNA
Elution volume200 µl - 1000 µl

Resources

试剂盒操作手册 (1)
For purification of DNA from human whole blood, buffy coat, cultured cells
其他资源 (1)
7 years storage at 2-8°C and -20°C
补充实验方案 (1)
This protocol is designed for purification of DNA from up to 10 ml clotted blood using the FlexiGene DNA Kit.
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)

Publications

The (TAAAA)n microsatellite polymorphism in the SHBG gene influences serum SHBG levels in women with polycystic ovary syndrome.
Ferk P; Teran N; Gersak K;
Hum Reprod; 2006; 22 (4):1031-6 2006 Dec 22 PMID:17189294
A functional SNP of interferon-gamma gene is important for interferon-alpha-induced and spontaneous recovery from hepatitis C virus infection.
Huang Y; Yang H; Borg BB; Su X; Rhodes SL; Yang K; Tong X; Tang G; Howell CD; Rosen HR; Thio CL; Thomas DL; Alter HJ; Sapp RK; Liang TJ;
Proc Natl Acad Sci U S A; 2007; 104 (3):985-90 2007 Jan 10 PMID:17215375
Lipopolysaccharide-activated CD4+CD25+ T regulatory cells inhibit neutrophil function and promote their apoptosis and death.
Lewkowicz P; Lewkowicz N; Sasiak A; Tchórzewski H;
J Immunol; 2006; 177 (10):7155-63 2006 Nov 15 PMID:17082633
Predictors of vitamin B6 and folate concentrations in older persons: the InCHIANTI study.
Gori AM; Sofi F; Corsi AM; Gazzini A; Sestini I; Lauretani F; Bandinelli S; Gensini GF; Ferrucci L; Abbate R;
Clin Chem; 2006; 52 (7):1318-24 2006 May 11 PMID:16690736
Past adult lead exposure is linked to neurodegeneration measured by brain MRI.
Stewart WF; Schwartz BS; Davatzikos C; Shen D; Liu D; Wu X; Todd AC; Shi W; Bassett S; Youssem D;
Neurology; 2006; 66 (10):1476-84 2006 May 23 PMID:16717205

FAQ

Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?

Yes, please follow the User-Developed Protocol 'Purification of DNA from clotted blood using the FlexiGene DNA Kit' (FG01).

 

FAQ ID -973
Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?

Yes. We have shown that DNA purified with the Flexigene DNA Kit is stable for at least 7 years when being stored at either 2-8°C or -20°C.  However, the DNA stability is dependent upon storage conditions.  The data is available here under Additional Resources.

 

FAQ ID -1317
Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?

Yes, we have the following protocols:

  • Isolation of genomic DNA from frozen clotted whole blood using the QIAGEN Genomic-tip 100/G (QG02). TEST

You will need to prepare the required buffers according to the recipes in Appendix A of the QIAGEN Genomic DNA Handbook, or you can purchase the Genomic DNA Buffer Set containing pre-made solutions. Alternatively, the QIAGEN Blood & Cell Culture Midi Kit, containing Genomic-tips 100/G and buffers, can be used.

  • Purification of archive-quality DNA from clotted whole blood using Clotspin Baskets and the Gentra Puregene Blood Kit (PG03).
  • Purification of archive-quality DNA from clotted whole blood using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit (PG04).
  • Purification of DNA from clotted blood using the FlexiGene DNA Kit (FG01).
FAQ ID -900. Test
What is the composition of Buffer FG3?
Buffer FG3, the hydration buffer supplied in the FlexiGene DNA Kit, is a 10 mM Tris-Cl solution at pH 8.5.
FAQ ID -356