QIAamp DSP DNA FFPE Tissue Kit

用于从石蜡包埋组织中纯化DNA

S_1084_5_GEN_V2

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QIAamp DSP DNA FFPE Tissue Kit (50)

Cat. No. / ID:  60404

For 50 DNA preps: QIAamp MinElute columns, Proteinase K, Buffers, and Collection Tubes (2 ml)
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QIAamp DSP DNA FFPE Tissue Kit 旨在用于体外诊断用途。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 从石蜡包埋组织中快速纯化DNA
  • 产量高且稳定
  • 去除污染物和抑制剂

Product Details

QIAamp DSP FFPE Tissue Kit利用硅胶技术从福尔马林固定、石蜡包埋组织中纯化DNA。QIAamp DSP DNA FFPE Tissue Kit专为处理福尔马林固定、石蜡包埋组织的实验室设计。

Performance

The QIAamp DSP DNA FFPE Tissue Kit使用QIAamp MinElute离心柱从福尔马林固定、石蜡包埋组织中纯化高品质DNA。可有效从福尔马林固定、石蜡包埋组织切片中纯化基因组DNA,无需过夜孵育。流程可部分去除DNA和福尔马林的交联,可提高产量和DNA品质,用于下游应用。

Principle

QIAamp DSP DNA FFPE Tissue Kit使用成熟的QIAamp MinElute技术从福尔马林固定、石蜡包埋组织中纯化基因组DNA。试剂盒包含的硅胶膜可灵活选择20到200 µl的洗脱体积。

特殊优化的裂解条件可从福尔马林固定、石蜡包埋组织切片中高效纯化基因组DNA,无需过夜孵育。蛋白酶K消化后升高温度孵育,可部分去除释放的DNA和福尔马林的交联,提高产量以及DNA品质。从福尔马林固定、石蜡包埋组织中纯化的DNA与从新鲜或冷冻样本中纯化的DNA相比,通常具有更低的分子量。片段化程度取决于样本类型、时间和固定条件。

Procedure

QIAamp DSP DNA FFPE Tissue Kit使用特殊的裂解条件从组织切片中释放DNA,并避免福尔马林与核酸交联产生的抑制作用。流程包括6个步骤:脱腊、裂解、加热、结合、洗涤和洗脱(参见"Procedure")。石蜡在二甲苯中溶解并被去除。样本在变性条件下裂解,用蛋白酶K短暂消化。90°C孵育,使福尔马林交联逆转。DNA结合到膜上,污染物被洗去。DNA在Buffer ATE中被洗脱,可即用于扩增反应或在–20°C保存。简便的QIAamp DSP DNA FFPE Tissue Kit操作流程非常适合同时处理多个样本。

Applications

QIAamp DSP DNA FFPE Tissue Kit专门用于从福尔马林固定、石蜡包埋组织中纯化DNA。

Resources

试剂盒操作手册 (2)
QIAamp DSP DNA FFPE Tissue Handbook_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
For the Directive 98/79/EC (IVDD) compliant kit (kit version 1)
产品介绍与指南 (1)
High-quality, nucleic acid purification for successful PCR and NGS experiments.
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
性能数据 (1)
QIAamp DSP DNA FFPE Tissue Performance Characteristics_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

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