ipsogen PML-RARA bcr3 Kit

采用real-time PCR对PML-RARA bcr3融合基因转录本进行定量检测

S_3180_IpsogenMLLRARAL_s

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ipsogen PML-RARA bcr3 Kit (24)

Cat. No. / ID:  672313

For 24 reactions: ABL Control Gene Standards, PML-RARA bcr3 Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix PML-RARA bcr3 Fusion Gene
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 灵敏、准确的定量检测试剂
  • 符合EAC要求的标准化试剂,结果具有可重复性
  • 即用型解决方案,便利的工作流程

Product Details

ipsogen PML-RARA bcr3 Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对PML-RARA bcr3融合基因转录本在总RNA中相对于ABL对照基因的表达进行可靠、灵敏的定量检测。

Performance

为确保高灵敏度,ipsogen PML-RARA bcr3 Kit已经过优化,使用预稀释的质粒标准品和引物探针混合液,检测PML-RARA bcr3和ABL转录本(参见Accurate detection of PML-RARA bcr3 fusion gene plasmid standardsReliable detection of ABL plasmid standards)。

Principle

ipsogen PML-RARA bcr3 Kit为即用型试剂盒,采用real-time PCR技术,检测PML-RARA bcr3融合基因转录本。该试剂盒可扩增并检测总RNA中相对于ABL对照基因表达的特定PML-RARA bcr3融合基因转录本(参见PML-RARA bcr3 gene transcriptABL control gene transcript)。该试剂盒灵敏度高,特异性强,可重复性好。ipsogen PML-RARA bcr3 Kit为PML-RARA bcr3融合基因提供5个标准品稀释液,为ABL提供3个标准品稀释液。使用标准品能够实现准确的定量检测。已与欧洲抗癌协会(EAC)联合对检测方法的标准化进行验证,ipsogen PML-RARA bcr3 Kit正是基于这种技术。

Procedure

第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增cDNA。ipsogen PML-RARA bcr3 Kit可对PML-RARA bcr3和ABL转录本进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

Applications

ipsogen PML-RARA bcr3 Kit能够对PML-RARA bcr3转录本相对于ABL对照基因的表达进行可靠、灵敏的定量检测。仅适用于科研。

Supporting data and figures

Resources

试剂盒操作手册 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks (1)