QIAsymphony DNA Investigator 试剂盒

用于在 QIAsymphony SP 仪器上从 1 至 96 个样本中自动纯化 DNA

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QIAsymphony DNA Investigator Kit (192)

Cat. No. / ID: 931436

用于 192 份 200 µl 案件和和参考样本制备：包括 2 个试剂条、2 个酶架及附件

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The QIAsymphony Investigator Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

从法医和人类身份鉴定样本中纯化
从痕量案件工作样本中获得更高效的产量
信噪比更高
新颖易用的预填充试剂卡夹
通过标准化处理实现高重现性

Product Details

QIAsymphony DNA Investigator Kit 能在 QIAsymphony SP 上自动纯化来自 1-96 个样本（如拭子、过滤器、案件或犯罪现场样本和血液）的基因组 DNA。纯化效率高，并且纯化后的 DNA 在下游分析（如定量 PCR 和 STR 分析）中表现良好，信噪比高。QIAsymphony DNA Investigator Kit 符合 ISO 18385 要求。

Performance

QIAsymphony DNA Investigator Kit 能高效纯化 DNA，即使样本材料数量极少也可以。由于兼具高达 1000 µl 的样本输入量和低至 30 µl 的洗脱体积，因此能高灵敏地检测多种类型的样本，包括分散型斑迹。

Principle

QIAsymphony DNA Investigator Kit 兼具硅基纯化基因组和线粒体 DNA 的速度和效率与磁珠微粒处理方便性（见“”图）。创新的即用型试剂卡夹预填充了磁珠和纯化程序所需的所有试剂，并由 QIAsymphony SP 自动打开，有助于将环境污染的风险降至最低（见“”图）。工作台设置迅速，可节省您的宝贵时间。只需将最多 2 个试剂卡夹放入“Reagents and Consumables”（试剂和耗材）抽屉。试剂卡盒可来自同一试剂盒或不同试剂盒，这样就可以在 96 个样本的同一运行过程中执行不同的纯化程序。只使用所选数量样本所需的试剂量，从而实现全面的成本控制。为了提高灵活性，DNA 可以在 30 至 400 µl 的水或 Buffer ATE（改良 TE 缓冲液）中进行洗脱。

See figures

磁微粒的高效处理。

预填充密封试剂卡盒。

Procedure

纯化操作程序旨在确保安全且可重复地处理宝贵或可能有传染性的样本，包含 4 个步骤：裂解、结合、清洗和洗脱。用户可根据方案在 30 µl 到 400 µl 水或改良 TE 缓冲液 (Buffer ATE) 范围内选择不同的洗脱体积。

由于可以使用 QIAsymphony DNA Investigator Kit 处理的样本类型可能差异很大，因此还有各种不同的预处理，其已针对特定样本类型进行了优化。样本在存在蛋白酶 K 和 Buffer ATL 的变性条件下进行裂解，总体积为 200 µl、500 µl 或 1000 µl。

为了应对法医样本的特殊挑战，我们开发了样本制备方案，以确保尽可能高的一次通过成功率。高级方案 (ADV) 采用加热结合步骤并延长结合时间，以最大限度地从最难处理的样本中获得纯净 DNA。高效 (HE) 方案解决了从接触样本和其他低模板样本中回收足够 DNA 的难题。TopElute 液体可最大限度地提高对这些疑难样本的灵敏度。下表介绍了适用于不同参考和案件工作样本的全套高效法医方案。

QIAsymphony DNA Investigator 方案
方案	裂解体积选项	增强结合	Eluate volume（洗脱液体积）	TopElute 液体
参比物质	200、500 µl	否	100–400 µl	否
案件工作	200、500、1000 µl	否	100–200 µl	否
Casework ADV	200、500、1000 µl	是	100–200 µl	否
Casework HE	200、500、1000 µl	否	30–80 µl	是
Casework HE ADV	200、500、1000 µl	是	30–80 µl	是

Applications

使用 QIAsymphony DNA Investigator Kit 获得的高质量 DNA 适合直接用于法医、生物防御、生物安全以及其他人类身份鉴定应用的诸如以下活动：

基因分型，包括指纹图谱和亲子鉴定分析
从痕量样本中纯化 DNA（例如，犯罪现场）
参考样本的常规分析

Supporting data and figures

磁微粒的高效处理。

QIAsymphony SP 以如下方式处理含有磁微粒的样本。受到杆套保护的磁杆进入含有样本的孔，然后吸附磁微粒。将磁杆及杆套置于另一孔之上，然后释放磁微粒。QIAsymphony SP 使用的磁头包含 24 个磁杆组成的阵列，因此最多可以同时处理 24 份样本。样本处理期间步骤 1 和 2 重复数次。

Specifications

Features	Specifications
Applications	STR 分析、（实时）PCR
Yield	< 3 µg
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	基因组 DNA、线粒体 DNA
Elution volume	参考样本：100-400 µl；案件样本：30-200 µl
Sample type	种类多样的法医和人类身份样本材料
Input volume	参考样本：200 或 500 µl 裂解物；案件样本：200、500 或 1000 µl 裂解物
Technology	磁珠技术

Resources

For use with software version 5.0

For use with software version 5.0 or higher

For use with software version 5.0

For use with software version 5.0 or higher

For use with software version 5.0

For use with software version 5.0 or higher

For use with software version 5.0

For use with software version 5.0 or higher

Advanced sample collection, automation and STR solutions for kinship testing

Streamline your workflow with QIAGEN solutions for human identity and forensic testing

Download Safety Data Sheets for QIAGEN product components.

High-throughput DNA sample prep using the QIAsymphony SP instrument: An overview of workflow optimization at Bode Cellmark Forensics.

For use with the QIAsymphony SP/AS instruments (software version 4.0)

QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0

Quality initiatives for human identity testing and forensics

For use with software version 4.0

This protocol has been adapted from the pretreatment of sexual assault samples and is for the lysis and extraction of DNA from a sperm pellet after differential separation.

High-throughput DNA sample prep using the QIAsymphony SP instrument: An overview of workflow optimization at Bode Cellmark Forensics.

Advanced sample collection, automation and STR solutions for kinship testing

Streamline your workflow with QIAGEN solutions for human identity and forensic testing

For use with software version 4.0

QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0

For use with software version 5.0

For use with software version 5.0 or higher

Quality initiatives for human identity testing and forensics

This protocol has been adapted from the pretreatment of sexual assault samples and is for the lysis and extraction of DNA from a sperm pellet after differential separation.

FAQ

For most sample types, incubating longer than specified in the pretreatment protocol for the QIAsymphony DNA Investigator Kit does not increase yields. However, lysates can be incubated overnight without negatively affecting the quality of the extracted DNA.

FAQ ID -2032

No. All solid materials (e.g., swabs, blood card punches, chewing gum) must be removed completely from the lysate before performing automated extraction on the
QIAsymphony SP. The QIAshredder can be used to increase sample recovery from some solid materials. For more information, see the corresponding pretreatment protocol.

FAQ ID -2027

User can choose elution with either buffer AVE or modified TE buffer known as buffer ATE.

* Buffer AVE contains RNase-free water with 0.04% NaN3 (Sodium azide).

* Buffer ATE contains 10 mM Tris-Cl pH 8.3, 0.1 mM EDTA and 0.04% NaN3 (Sodium-azide).

FAQ ID - 3387

No. The specified lysate volumes are optimal for QIAsymphony DNA Investigator Kit protocols. Lower volumes (150 µL,400 µL, 900 µL, respectively) can be used.

However, to maximize yields, we recommend transferring as much of the lysate as possible.
The QIAshredder can be used to increase recovery from some sample types. For more information, see the corresponding pretreatment protocol.

FAQ ID -2031

The large-volume protocol of the QIAsymphony DNA Investigator Kit is intended for samples that can not be submerged completely during lysis in volumes less than 1 ml, as well as samples that are very absorbent.

FAQ ID -2034

QIAsymphony DNA Investigator Kit protocols are optimized for maximum sensitivity in extraction of typical forensic-casework and reference sample types, which usually have low DNA content. Due to optimized bead capacity, maximum DNA yields are approximately 3 µg.

FAQ ID -2028

No. Carrier RNA is only used in casework protocols for the QIAsymphony DNA Investigator Kit to enable efficient extraction of minute quantities of DNA. Carrier RNA is not used in reference protocols.

FAQ ID -2033

To ensure performance with the QIAsymphony DNA Investigator Kit, lysis volumes have been optimized according to sample type. In general, large or absorbent samples require larger buffer volumes than small and non-absorbent samples.

FAQ ID -2035