Will pipetting error affect the EpiTect Methyl qPCR Array results?
FAQ ID -2741

The passive reference dyes in the PCR SYBR Green master mixes, such as ROX and Fluorescein, are used by the real-time PCR instruments to normalize variation from well to well. Therefore, these systems tolerate volume variations caused by pipetting error or evaporation. Even a 20% pipetting error causes only 0.05-cycle differences in Ct values.