Measurable residual disease (MRD) is the most important post-treatment predictor of outcome in patients with adult myeloid leukemia (AML). While flow cytometry-based methods for MRD detection are well-established clinically, molecular methods of MRD detection are still in research development. The use of digital PCR for highly sensitive monitoring of AML-associated mutations in patients on a variety of treatment plans will be discussed, as well as its predictive value for relapse. Bone marrow and cell-free DNA-based MRD will be explored. We will also  demonstrate the value of multiplexing assays for simultaneous detection of multiple mutations in limiting patient samples.

Measurable residual disease (MRD) is the most important post-treatment predictor of outcome in patients with adult myeloid leukemia (AML). While flow cytometry-based methods for MRD detection are well-established clinically, molecular methods of MRD detection are still in research development. The use of digital PCR for highly sensitive monitoring of AML-associated mutations in patients on a variety of treatment plans will be discussed, as well as its predictive value for relapse. Bone marrow and cell-free DNA-based MRD will be explored. We will also  demonstrate the value of multiplexing assays for simultaneous detection of multiple mutations in limiting patient samples.