The fine art of dPCR – From template to validation
About the session
In 2009, a group of qPCR experts published the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines for reproducible experiments. Those guidelines shaped present-day qPCR, which is considered a gold standard technique in molecular biology. Fast forward to 2013, dPCR also took advantage of the publication of the Minimum Information for Publication of Quantitative Digital PCR Experiments (dMIQE) guidelines to ensure global standardization. A new version of the dMIQE guidelines was published in 2020, considering the increasing number of applications and the introduction of new platforms.
In this session, we will address practical considerations for:
- Template treatment
- Assay design
- Thermal cycling protocols
- Assay validation
John Paul Chuckalovcak is broadly trained in molecular biology with expertise in analytical test optimization and validation. In his 10 years of experience with digital PCR, he has supported the technology’s integration into academic, government, biotech and commercial operations. At QIAGEN, he offers consultation to research and applied testing laboratories in North America to facilitate the adoption of dPCR for a breadth of applications.