Real-time qPCR

Ensuring quality qPCR

The versatility of quantitative, real-time PCR (qPCR) resulted not only in its ubiquity as a research tool, but also in an abundance of questionable qPCR findings in scientific journals. Without experimental transparency, it’s impossible to assess the integrity of a study or its conclusions. Thus in 2009, the MIQE guidelines were established to address this problem and define the standard for designing and reporting qPCR experiments.

Good qPCR assays display high accuracy, dynamic range, reliability, reproducibility, sensitivity and specificity. In our experience, the primer design algorithm and the reaction buffer conditions must work together to provide the best results. Many researchers design their own assays without full knowledge of the critical design aspects, and often unknowingly compromise their results.

QIAGEN qPCR solutions take the guesswork out of your experiments. Our broad selection of qPCR kits and an unparalleled choice of predesigned assays and panels and the option to design your own using our advanced algorithms ensure quality qPCR results time and again.

Streamline your research planning with GeneGlobe​
Explore targets and pathways, find or customize the optimal PCR assays and panels, get quick insights into your data and plan follow-up studies – in just one tool!​
There’s no compromise in qPCR

QuantiNova kits represent today’s most advanced portfolio of real-time PCR and RT-PCR kits for gene expression analysis, gene quantification and pathogen detection. A unique combination of in-process controls removes variables and minimizes human error, so your experiments are bound to work. Simple and fast procedures increase your efficiency, saving you time and money. Plus, flexibility in detection chemistry and full real-time cycler compatibility mean there’s no need to compromise.

Not sure which PCR products to pick?

This smart online tool can help you find the right products and instruments for every step of your protocol.

Workflow Configurator, wfc, Luna

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Does your project have unique requirements?
Our complete range of real-time PCR solutions has you covered. Contact our experts to find the best tool for you!

Guides and tips for great results​

Unparalleled range of assays and panels

Quantitative real-time RT-PCR remains the method of choice for quantification of RNA transcripts, providing flexibility and speed for time-critical assays. Whether you’re assessing miRNA, mRNA or lncRNA expression or verifying the results of NGS studies, our qRT-PCR solutions offer significant benefits for your work.

  • High sensitivity: Robust chemistry and an extraordinary offering of assays designed to detect and quantify even the rarest target efficiently.
  • High specificity: Proprietary LNA oligo design algorithms and bench verification ensure on-target amplification from a single assay to a full panel.
  • Large dynamic range: Whether you have unlimited or precious amounts of total RNA, robust or rare targets, one assay or thousands, our tailored solutions enable detection across the entire spectrum.
  • Choice of detection chemistry: Our newest assays and panels are available in both SYBR® Green and hydrolysis probe formats, so you can achieve excellent results no matter which technology you prefer.
What does LNA do for your PCR?

Our newer qPCR assays and panels are enhanced with locked nucleic acids (LNA). This increases primer binding affinity, dramatically increasing the assay specificity and sensitivity. This higher affinity also allows us to design shorter primers and probes, which are more flexible to place on the target, making it easier to achieve an optimal assay design that minimizes non-specific amplification. Plus, varying the number of LNA bases lets us normalize the primer Tm so that all PCR primers have the optimal Tm for the specified conditions, regardless of the target GC content.

Want to know more? Visit our LNA technology center to learn how LNA can enhance your PCR-based miRNA profiling and gene expression analyses!

Probe-based miRNA profiling for demanding applications

You asked, and we delivered! The new miRCURY LNA miRNA Probe PCR system uses hydrolysis probe-based detection to bring even higher specificity and robustness to your demanding microRNA analyses. LNA enhancement allows us to design very short, sequence-specific and nonoverlapping primers and probes with extremely high affinity and specificity. This just isn't possible with conventional oligos. Plus, a streamlined workflow gives robust results without pre-amplification or bias.

miRNA profiling for SYBR® friends

Our SYBR® Green-based miRCURY LNA miRNA PCR assays and panels offer a cost-effective alternative to probe-based detection. Some researchers worry about getting false positives when using intercalating dyes, but that problem comes from sub-optimal primer design and PCR conditions. The miRCURY LNA miRNA PCR system includes highly specific, well-designed primers that are already optimized for use with the miRCURY LNA RT Kit and the miRCURY LNA SYBR® Green PCR Kit. You can count on extremely sensitive and specific miRNA quantification.

The new standard in mRNA/lncRNA profiling

qPCR-based gene expression analyses and verification of RNA-seq data require quick access to highly specific and sensitive assays for the targets of interest. The new QuantiNova LNA PCR and Probe PCR portfolios give you just that. Predesigned and custom assays and panels provide the option of SYBR® Green or probe-based detection. Short, LNA-enhanced primers and probes provide greater sensitivity and flexibility in assay design than traditional oligos, making the systems ideal for a broad range of research needs.

Raise your qPCR workflow to the next level

It is increasingly challenging to find the right real-time PCR cycler for your laboratory’s application requirements. For best results, you need low operating costs, high throughput, the ability to multiplex with fast analysis times and reliable reproducibility. As the list of features and specifications grows, an understanding of some of the key performance benefits such as precision, uniformity, dynamic range and resolution becomes critical.

Featured resources​

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