Make a smooth transition to superior miRNA PCR

We aim to provide the most innovative and high-quality products to help scientists overcome challenges and advance their research. Accordingly, we will stop producing miScript miRNA PCR products on May 1, 2021. miScript PCR products will be available after that date as long as our stocks last, and we recommend that miScript PCR users switch to our miRCURY LNA miRNA PCR systems.

qPCR-based miRNA analysis presents special challenges: their low abundance in common sample types often necessitates a preamplification step, and their short sequences make designing highly specific primers and probes difficult. Our miRCURY LNA miRNA PCR systems eliminate these issues and deliver robust and highly sensitive miRNA profiles without preamplification, even for the most demanding applications.

miRCURY LNA miRNA PCR provides several unique advantages:

  • Unmatched sensitivity with quantification from just 1 miRNA copy
  • Accurate detection from low miRNA content samples, such as serum/plasma, FFPE sections and LCM samples, without preamplification
  • High specificity and discrimination of closely related miRNAs
  • Easy online customization of assays and panels
  • A fast and simple protocol that takes just 2–3 hours

miScript PCR kits to be phased out and their recommended successors 

To-be-phased-out product Recommended successor product for SYBR Green detection Recommended successor product for probe-based detection
 Product name Cat. No. Product name Cat. No. Product name  Cat. No.
miScript Reagents
miRCURY LNA PCR Reagents

miRCURY LNA Probe PCR Reagents
miScript II RT Kit (12)
218160 miRCURY LNA RT Kit 339340 miRCURY LNA RT Kit 339340
miScript II RT Kit (50)
218161 miRCURY LNA RT Kit
339340 miRCURY LNA RT Kit 339340
miScript SYBR Green PCR Kit (200)
218073 miRCURY LNA SYBR Green PCR Kit (200) 339345 miRCURY LNA Probe PCR Kit (200) 339371
miScript SYBR Green PCR Kit (1000) 218075 miRCURY LNA SYBR Green PCR Kit (600)
339346 miRCURY LNA Probe PCR Kit (800)
339372
miScript SYBR Green PCR Kit (2000) 218076 miRCURY LNA SYBR Green PCR Kit (4000)
339347 miRCURY LNA Probe PCR Kit (4000)
339373
miScript Assays
miRCURY LNA miRNA PCR Assays
miRCURY LNA miRNA Probe PCR Assays
miScript Primer Assay (100) 218300

miRCURY LNA miRNA PCR Assay (200)
or
miRCURY LNA miRNA Custom PCR Assay (200)

339306
or
339317

miRCURY LNA miRNA Probe PCR Assay (200)

or

miRCURY LNA miRNA Custom Probe PCR Assay (200)

339350

or

339351

miScript Panels/Arrays
miRCURY PCR Panels   miRCURY Probe PCR Panels  
miRNome miScript miRNA PCR Array 331222

miRCURY LNA miRNA miRNome PCR Panels

FIND YOUR PANEL

339322 miRCURY LNA miRNA Probe PCR miRNome Panel Contact us
miScript miRNA PCR Pathway Array 331221

miRCURY LNA miRNA Focus PCR Panels

FIND YOUR PANEL

339325 miRCURY LNA miRNA Probe PCR Focus Panel Contact us
miScript miRNA HC PCR Array 331223

miRCURY LNA miRNA miRNome PCR Panels
or
miRCURY LNA miRNA Focus PCR Panels

FIND YOUR PANEL

339322
or
339325

Varies Contact us
miScript miRNA QC PCR Array 331292 miRCURY LNA miRNA QC PCR Panel 339331 Varies Contact us
Custom miScript miRNA PCR Array 331231 miRCURY LNA miRNA Custom PCR Panel 339330 miRCURY LNA miRNA Probe PCR Custom Panel Contact us
Other specialized miScript Kits  
 
 
miScript Plant RT Kit (50) 218761 Contact us for best solution   Contact us for best solution  
miScript Plant RT Kit (96)
218762 Contact us for best solution
  Contact us for best solution  
miScript Single Cell qPCR Kit (24)
331053 Contact us for best solution
  Contact us for best solution  
miScript PreAMP Pathway Primer Mix
331241
Learn more below   Learn more below  
miScript PreAMP Pathway HC Primer Mix 331242 Learn more below   Learn more below  
miScript PreAMP miRNome Primer Mix 331251 Learn more below   Learn more below  
miScript PreAMP Custom Primer Mix 331260 Learn more below   Learn more below  
miScript PreAMP PCR Kit (12) 331451 Learn more below   Learn more below  
miScript PreAMP PCR Kit (60) 331452 Learn more below   Learn more below  
miRCURY LNA PCR – when sensitivity, accuracy and flexibility really matter

The miRCURY LNA miRNA PCR systems are available in both SYBR® Green- and probe-based versions and combine the speed and convenience of a universal reverse transcription reaction with the sensitivity and specificity of LNA-enhanced qPCR primers. The easy workflow is designed for high-performance miRNA profiling and delivers fast, accurate results for all sample types – even those with low RNA content.

Tailor the experimental setup to your specific needs using individual assays or fully flexible custom panels. Using the same cDNA reaction, shift seamlessly between individual predesigned or custom primer assays or panels. Our extraordinary offering of predesigned assays and panels allows you to detect and quantify even the rarest target with highest efficiency.

Accurate quantification without preamplification

Quantification of low-abundance miRNAs by qPCR usually requires preamplification, but the miRCURY LNA miRNA PCR systems provide accurate results without this extra step. What’s the secret? miRCURY PCR assays and panels contain optimally positioned locked nucleic acid (LNA) bases. LNA-enhanced oligos exhibit increased primer binding affinity, allowing the design of very short primers (and probes) that are both miRNA-specific and short enough to prevent overlap when binding to the target sequence. This just isn’t possible with traditional oligos. As a result, we are able to design an optimal assay to detect any biologically relevant miRNA with the highest specificity and sensitivity – without the need for preamplification.

The miRCURY LNA miRNA PCR system has already helped many scientists advance their research. Want to know more? This team used our system to discover new miRNA biomarkers for early diagnosis of osteoporosis. These researchers use it for detecting plasma miRNA signatures associated with phenotypes related to common human diseases. Our Guidelines for Profiling Biofluid miRNAs discuss how to monitor the efficiency of your PCR with RNA spike-ins and include other tips and tricks for successful PCR analysis of low miRNA content biofluid samples without preamplification.

If you have a specialized application that does require preamplification, please contact our experts for further support.

Your choice of detection chemistry

SYBR® Green-based assays are flexible to design and offer lower assay setup and running costs, but many researchers are concerned about false positives since the dye binds to all double-stranded DNA. False positives are usually due to sub-optimal PCR conditions, not the detection chemistry. Plus, SYBR® Green detection allows melting curve analysis immediately following PCR, so you can confirm high reaction specificity and rule out formation of primer–dimers or non-specific amplicons.

Hydrolysis probes provide highly sensitive and robust detection, because only the desired PCR product is detected. However, assay design can be a challenge for short targets like miRNA, and designing conventional DNA oligo primers and probes is often impossible. Plus, amplification artifacts can compete for reaction components, compromising assay sensitivity and efficiency, and reducing the yield of your desired PCR product.

Both methods can provide excellent results, as long as you use a highly specific PCR system with well-designed primers (and probes) and reaction conditions that are optimized to ensure that only the desired targets are amplified. The miRCURY LNA miRNA PCR assays and panels are enhanced with LNA, allowing us to create very short primers (and probes) that are spatially separated and still display extremely high affinity and specificity. miRCURY LNA PCR ensures a successful analysis every time, without primer–dimer interference or false positive results.

miRCURY LNA miRNA PCR with SYBR® Green detection

The miRCURY LNA miRNA PCR system includes highly specific, well-designed primers that are already optimized for use with the miRCURY LNA RT Kit and the miRCURY LNA SYBR® Green PCR Kit. This cost-effective alternative to probe-based detection provides extremely sensitive and specific quantification of any miRNA target.

icon-kit-tube
Try a miRCURY LNA miRNA PCR Starter Kit
Includes everything you need to get started – two assays of your choice, a spike-in control primer set, one candidate endogenous control primer, plus reagents.
miRCURY LNA miRNA PCR with hydrolysis probe detection

For demanding miRNA analysis that require even higher specificity and robustness, we recommend the miRCURY LNA miRNA Probe PCR system. LNA enhancement allows us to design very short, sequence-specific and non-overlapping primers and probes that display extremely high affinity and specificity. This just isn't possible with conventional oligonucleotides.

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