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Your path to cell-free DNA analysis
Comprehensive workflow solutions starting with blood collection and stabilization along the molecular analysis workflow from dedicated cfDNA preparation to integrated data analysis solutions for various applications by next-generation sequencing or digital PCR, scalable to your sample volume, throughput or outsourcing needs.
Products along the workflow
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Learn more about the options available to you and consult with our experts.
A range of different manual or automated cfDNA isolation systems are available to suit different starting volume, processing time and throughput needs. To help you choose the best combination of products to isolate cfDNA for your applications, see the graphics, or download the brochure that also includes information on cfDNA analysis by dPCR and next-generation sequencing and data interpretation systems.
Select products for research applications
Select products for diagnostic applications
cfDNA isolation from large starting volumes
The concentration of cfDNA in biological fluids such as plasma or urine is usually low and varies considerably between individuals, usually from 1–50 ng/ml. Typically, input volumes of 4 ml are used, which corresponds to the total plasma volume from one blood collection tube. Depending on the target DNA, the application, and the sensitivity of the downstream assay, the sample input volume needed can vary from 1 to 10 ml. We provide a range of cfDNA purification kits that offer fast workflows and unmatched cfDNA concentrations from varying sample inputs for high sensitivity in downstream NGS and PCR analyses.
More information on instruments suitable for automated cfDNA isolation
Imagine analyzing a cfDNA eluate containing a very rare event. You might want to get the maximum possible input into the cell-free DNA analysis workflow. Combine up to 8 ml plasma sample processing on EZ2 Connect with high-volume eluate loading on QIAcuity.
Have you considered the power of two?
EZ2 Connect and QIAcuity for cfDNA analysis
If you isolate cfDNA from a larger volume of serum or plasma and then use large-volume eluate loading with a highly sensitive detection method like digital PCR, you can load more and see more. Processing larger volumes gets more template cfDNA and dPCR will detect more mutations. You can achieve this goal by combining EZ2 Connect for easy a sample processing with the QIAcuity dPCR system.
You can now combine a fully automated and convenient high-volume sample processing on the EZ2 Connect with fast and sensitive ultra-low mutation detection using digital PCR on the QIAcuity.
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See for yourself
The best way to see the power of two is to try it out yourself. Complete the form to request a demo in your lab.
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Cell-free DNA analysis – status and outlook
Ellen Heitzer presents several studies evaluating various workflows for cfDNA analysis. Issues hampering the clinical implementation of cfDNA analysis and possible ways forward are discussed.
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Standardization in cell-free DNA sample processing
A deep dive into cell-free DNA: learn about a true walkaway solution for automated isolation from up to 8 ml. And hear from Constanze Kindler, Ph.D. about challenges and new solutions for analyzing cfDNA from up to eight milliliters of blood.
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cfDNA collection/stabilization and sample preparation
Martin Schlumpberger and our panel of experts answer your questions on how to collect, stabilize and process your samples when working with cfDNA. Get answers, tips and tricks to ensure a successful start to your analysis.
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cfDNA Digital PCR
Özlem Karalay and our dPCR experts dive deep into the exciting analysis opportunities offered by dPCR technologies. Check out how sensitive and accurate your cfDNA analysis can be.
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cfDNA NGS (SNV, CNV, methyl markers) and data analysis
Want to use the power of NGS to analyze your samples? Peter Hahn and our next-generation sequencing experts answer all your questions in this final panel discussion on cfDNA analysis.
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Cell-free DNA analysis – status and outlook
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Standardization in cell-free DNA sample processing
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cfDNA collection/stabilization and sample preparation
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cfDNA NGS (SNV, CNV, methyl markers) and data analysis
Quick answers and insights
