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Digital PCR

The new absolute in PCR – Digital PCR

Finding a needle in a haystack

Researchers performing sensitive applications such as copy number variation and rare target detection often find themselves struggling to identify faint genetic signals against a strong background, especially when it is a single positive lost in a dense pool of negatives. Finding the rare allele or mutant sequence is a typical needle in a haystack problem! Digital PCR (dPCR) is a technique where the sample is partitioned into a large number of individual reactions. This approach makes it surprisingly easy to detect the single positive. It is in such instances where the lower limit of detection excludes standard real-time quantitative PCR as a viable solution.

At QIAGEN, our mission is to empower you with everything you need to overcome challenges in real-time and digital PCR research applications. In the end, finding a needle in a haystack might not remain as challenging as it sounds.

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Realize the full potential of digital PCR

Find the top applications to be covered by our new assays.

Video recorded prior to COVID-19
If your future is digital, why shouldn't your PCR be, too? Imagine keeping the familiarity and ease of qPCR but gaining the higher sensitivity and precision of digital PCR, without prolonging the time to scientific insight. The QIAcuity, QIAGEN's fully integrated nanoplate-based digital PCR system, has been designed with your research needs and the limitations of the currently available methods in mind. It's time to simplify the transition from qPCR and prepare your critical research applications for the changing landscape of digital PCR.

Advantages over droplet digital PCR technology

The nanoplate-based technology offers significant benefits over digital droplet PCR (ddPCR). These include:

• Fixed partitions prevent variation in size and coalescence
• Sealed nanoplates prevent well to well contamination
• Faster readout possible due to simultaneous reading of all partitions of a sample
• User-friendly, familiar plates are easy to pipet just like for qPCR
• Plates are amenable to front-end automation

Read more about dpcr vs ddpcr

Video recorded prior to COVID-19
It is easy to use the QIAcuity
Transition into a simple and rapid digital PCR workflow.
New to dPCR?
If you’re ready to enter the world of dPCR, but don’t know where to start, this educational hub covers key topics designed for beginners.