QIAwave DNA/RNA Mini Kit

More eco-friendly alternative to our AllPrep DNA/RNA Mini kit for simultaneous purification of DNA and RNA from cells and tissues

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Get in touch with our team today and request a quote for your QIAwave DNA/RNA Mini Kit (50) trial kit.
QIAwave DNA/RNA Mini Kit (50)

Cat. No. / ID:  80504

For 50 minipreps: AllPrep DNA Spin Columns, RNeasy Mini Spin Columns, Waste Tubes, RNase-Free Water and Buffers
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NOK 5,305.00
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The QIAwave DNA/RNA Mini Kit (50) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Want to try this solution for the first time?
Get in touch with our team today and request a quote for your QIAwave DNA/RNA Mini Kit (50) trial kit.

Features

  • High-quality DNA and RNA from the cells and tissues
  • DNA/RNA quality and performance identical to the AllPrep DNA/RNA Mini Kit
  • 52% less plastic and 58% less cardboard compared to the AllPrep DNA/RNA Mini Kit
  • Reusable Waste Tubes made from 100% post-consumer recycled plastic
  • Buffer concentrates that use up to 90% less plastic than our standard buffer bottles

Product Details

The QIAwave DNA/RNA Mini Kit is a more eco-friendly version of our standard AllPrep DNA/RNA Mini Kit designed for purifying both genomic DNA and total RNA from the same cell or tissue sample. Since there is no need to divide the sample into two for separate purification procedures, maximum yields of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately and ready to use in any downstream application.


The QIAwave DNA/RNA Mini Kit uses 52% less plastic and 58% less cardboard than our standard kit and offers Waste Tubes made from 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers also come as concentrates, reducing the amount of plastic by up to 90% per bottle. To save paper, there are no printed protocols in the kit. Instead, you can download the protocols you need from the resources list or by simply scanning the QR code inside the box lid. So, while the kit packaging and components of our QIAwave DNA/RNA Mini Kit may look different, it’s as easy to use as the AllPrep DNA/RNA Mini Kit, and the chemistry and performance are identical.


Please be aware that you will need sterile glass bottles to store the reconstituted buffers.

 

Performance

The QIAwave DNA/RNA Mini Kit gives you highly reproducible yields of DNA and total RNA from cells and tissues.  The performance of our QIAwave AllPrep DNA/RNA Mini Kit and AllPrep DNA/RNA Mini Kit is identical because the chemistry is the same. (see figure “ DNA yields obtained with QIAwave DNA/RNA Mini Kit” and figure " RNA yields obtained with QIAwave DNA/RNA Minit Kit"). Total RNA isolated with the QIAwave DNA/RNA Mini Kit results in similar downstream RT-qPCR performance as total RNA isolated with the AllPrep DNA/RNA Kit. (see figure: RT-qPCR performance of total RNA isolated with the QIAwave AllPrep DNA/RNA Mini Kit from cells and tissue samples)

We have also compared DNA and RNA yield obtained with the QIAwave DNA/RNA Mini Kit where the buffer was prepared by pouring or pipetting and with the AllPrep DNA/RNA Mini Kit using standard buffers. All methods result in comparable DNA and RNA yields as shown in the figure “ Handling of buffer concentrates”.

We’ve also shown that the kit outperforms kits from alternative suppliers with regards to DNA RNA separation (see figure "  QIAwave DNA/RNA Mini Kit RT-qPCR performance").

DNA and RNA isolated with the QIAwave DNA/RNA Mini Kit showed high linearity in downstream dPCR over a wide range of input materials as shown in figure “ Linearity of dPCR copies using DNA from various starting amounts of tissue” and “ Linearity of dPCR copies using RNA from various starting amounts of tissue.”

 

See figures

Principle

The QIAwave DNA/RNA Mini Kit is designed to purify both genomic DNA and total RNA from the same cell or tissue sample. Since there is no need to divide the sample into two for separate purification procedures, maximum yields of DNA and RNA can be obtained. Efficient purification of high-quality DNA and RNA from different tissue types is achieved without the need for additional RNase or DNase digestion. The purified DNA and RNA are eluted separately and ready to use in any downstream application. 

Procedure

A simple spin-column workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart “ QIAwave DNA/RNA procedure”). Both cultured cells and easy-to-lyse tissues can be processed. Genomic DNA is purified using the novel AllPrep DNA spin column, and total RNA is purified using the RNeasy Mini spin column.

When disrupting and homogenizing tissues in Buffer RLT Plus (supplied with the QIAwave DNA/RNA Mini Kit), excessive foaming may occur. This foaming is substantially reduced by adding Reagent DX (supplied separately) to Buffer RLT Plus at a final concentration of 0.5% (v/v) before starting disruption and homogenization. Reagent DX has been carefully tested with the kits, and has no effect on RNA purity or on downstream applications such as real-time RT-PCR. Buffer RLT Plus containing Reagent DX can be stored at room temperature (15–25ºC) for at least 9 months.


QIAwave DNA/RNA Mini Kit is part of QIAGEN's solution for preparing multiple analytes from the same sample. These include Allprotect Tissue Reagent, which stabilizes DNA, RNA, and protein in tissues, and RNAprotect Cell Reagent, which stabilizes DNA and RNA in cells. Both reagents deliver immediate stabilization at room temperature. For tissues, TissueRuptor and TissueLyser systems provide fast disruption at low- to high-throughputs.


The QIAwave DNA/RNA Mini Kit can be automated on the QIAcube Connect using the AllPrep DNA/RNA Mini Kit protocols. 

 

See figures

Applications

The purified genomic DNA has an average length of 15–30 kb, depending on homogenization conditions, and is suited for any application, including next-generation sequencing, Southern-, dot-, and slot-blot analyses; and PCR, multiplex PCR and dPCR.


The purified total RNA can be used in any application, such as RNA-seq RT-PCR, real-time RT-PCR and dPCR; differential display; cDNA synthesis; northern-, dot-, and slot-blot analyses; and microarrays.

 

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, real-time PCR, dPCR, microarray, blotting
TechnologySilica technology
FormatSpin column
Sample typeCells and tissue
ProcessingManual (centrifugation) and automated (QIAcube Connect)
Sample amount1 x 107 cells or 30 mg tissue
Time per run or per prep35 minutes
Elution volume100 µl

Resources

Quick-Start Protocols (1)
Safety Data Sheets (1)
Kit Handbooks (1)

FAQ

I don’t have any glassware in the lab, is the QIAwave kit still a good option for me?
The concept of QIAwave includes the reconstitution of functional buffers. We recommend the use of glass bottles for this procedure. Glass bottles are easier to clean, sterilize and reuse than plastic bottles, further reducing the plastic footprint of the kit. If you do not have the option of using clean glass or plastic bottles, we recommend using our legacy kits. 
FAQ ID - 3989

Can the QIAwave kits be recycled?
We provided an infographic describing the composition of most of our purification kits. You can use the information provided as a guide to recycle kit components and use it to reduce plastic waste in your laboratory. Depending on the specific kit and application, certain kit components may contain or come into contact with chemicals and biological samples, in this case, the components should be disposed of according to local guidelines and regulations. You can find more information on More Sustainable Products (qiagen.com).
FAQ ID - 3992
What water should I use to prepare the buffer concentrates?
We recommend using highly pure water for reconstitution. Ultrapure water (such as the one from MilliQ system, also known as type 1 water) with a resistivity of 18.2 MΩ-cm at 25°C can be used. In case you do not have access to type 1 water, QIAGEN offers Nuclease-Free Water (5 L, cat. No. 129117); and Nuclease-Free Water (1000 mL, cat. No. 129115). It is important that you do not use tap water as this may interfere with the extraction of the target analyte. 
FAQ ID - 3986

Is there a way to compare the environmental impacts of the kit?
In partnership with My Green Lab, we were able to assess the environmental impact of the kits. My Green Lab ACT (accountability, consistency and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria. The products are scored from 1 to 10, except for energy and water consumption which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental factor.  
FAQ ID - 3991

Does the reuse of the Waste Tubes increase the risk of cross-contamination?
No, we were able to prove experimentally that cross-contamination does not occur through the reuse of the Waste Tubes. The Waste Tubes are used to collect the flow-through from the lysis and washing steps, where the flow-through is discarded afterwards. If the flow-through is to be used for further processing, we recommend the use of a Collection Tube
FAQ ID - 3988

Are the QIAwave kits based on a different chemistry than the legacy kits?
No, the chemistry between the QIAwave kits and the legacy kits is the same, and so is the performance. The QIAwave buffers come as concentrates, reducing the amount of plastic per bottle while maintaining the same functionality after reconstitution. The advantage of the QIAwave kits is the reduction of materials used for the kits, such as plastics, cardboard and paper. In addition, the QIAwave kits contain Waste Tubes made from 100% post-consumer plastic. 
FAQ ID - 3990

What effect does homogenization have on DNA yield and integrity when using QIAwave DNA/RNA Kits?
The yield and integrity of genomic DNA depend on the disruption and homogenization method used with the QIAwave DNA/RNA kits. 
Homogenization with the TissueRuptor II (or other rotor-stator homogenizer) or the TissueLyser III results in greater DNA fragmentation (depending on homogenization time and intensity). However, shorter DNA fragments are easier to elute, resulting in higher DNA yields.
FAQ ID - 3985

What is the new Waste Tube made of?
The new Waste Tube is made from recycled plastic recovered from post-consumer plastic waste. Due to the slight composition differences of the raw material, the color of the tubes may differ from lot to lot. However, this has no effect on its intended use of collecting flow-through from sample binding and membrane washing. After each step, the flow-through is discarded, and the Waste Tube can be reused. The Waste Tube is only used to process waste and should never come into direct contact with the analyte of interest. For detailed instructions, you can watch our instructional video at www.qiagen.com/qiawavewastetube
FAQ ID - 3987