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Plasmid Selection Guide - QIAGEN Large-Construct Kit

QIAGEN Large-Construct Kit For purification of up to 50 µg BAC, PAC and P1 DNA or up to 200 µg cosmid DNA, free of genomic DNA Format Gravity-flow anion-exchange column Applications PCR/Sequencing In vitro transcription/translation Cloning BAC/PAC prep Transfection of robust cells most cells primary/sensitive cells Equipment required Centrifuge Specifications Available kit scale Culture volume DNA yield Processing time N/A 500 ml <200 µg 300 min

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