Plasmid Selection Guide - QIAprep® Spin Miniprep Kit

QIAprep® Spin Miniprep Kit For easy purification of up to 20 µg molecular biology-grade plasmid DNA in spin-column format Format Silica-membrane spin column* Applications PCR/Sequencing In vitro transcription/translation Cloning BAC/PAC prep Transfection of robust cells most cells primary/sensitive cells Equipment required Centrifuge, vacuum manifold or QIAcube® * Specifications Available kit scale† Culture volume‡ DNA yield Processing time Mini <5 ml <20 µg 30 min High-Yield Protocol >5 ml ≤50 µg 30 min * Automatable on the QIAcube. † 96-well format is also available for molecular biology-grade plasmid. ‡ Culture volumes when using a high-copy vector.

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