Plasmid Selection Guide - EndoFree® Plasmid Kits

EndoFree® Plasmid Kits For purification of up to 10 mg endotoxin-free advanced transfection-grade plasmid or cosmid DNA Format Gravity-flow anion-exchange column Applications PCR/Sequencing In vitro transcription/translation Cloning BAC/PAC prep Transfection of robust cells most cells primary/sensitive cells Equipment required None for Midi and Maxi QIAvac HiSpeed LS Vacuum manifold for Mega and Giga Specifications Available kit scale Culture volume* DNA yield Processing time Maxi 100 ml <500 μg 150 min Mega 500 ml <2.5 mg 220 min Giga 2.5 l <10 mg 310 min * Culture volumes when using a high-copy vector.

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