samples (250 mg each) were prepared using
sample preparation solutions
and compared to the new
DNeasy PowerSoil Pro Kit.
DNA purity was depicted
via UV measurements and
the analysis of ratios
removal was visualized with an inhibitor-sensitive
PCR with an internal control
spiked with eluates from
the PureLink Microbiome
DNA Purification Kit and
the DNeasy PowerSoil
Pro Kit. Eluate-free
reactions served as
internal control, C. DNA extracted with the DNeasy PowerSoil Pro Kit shows an increased purity compared with other sample preparation kits with no inhibition of PCR.
Gel A shows 1.8 ml of Candida albicans fungal culture processed with the DNeasy PowerSoil Kit (a) compared with the DNeasy PowerSoil Pro Kit (b). Gels B and C
contain various soil samples (250 mg). Gel B was processed with the original DNeasy PowerSoil Kit, while Gel C was
processed using the DNeasy PowerSoil Pro Kit. As evidenced by the stronger gel bands, a greater DNA yield was isolated from the sample using the DNeasy PowerSoil Pro Kit.
DNA prepared from soil samples was isolated
with different methods and enriched. Analysis of the 16S rRNA-genes was done using the QIAseq 1-Step Amplicon Kit and data analysis was done using the Microbial Genomics Pro suite (CLC workbench). Alpha diversity was determined by total
number of bacterial operational taxonomic units (OTUs). The greatest diversity was seen in the samples processed with the DNeasy PowerSoil Pro Kit.
250 mg of the three different soil types were prepared using commercially available sample preparation solutions and compared to the new DNeasy PowerSoil Pro Kit. The DNeasy PowerSoil Pro Kit out-performed other available kits, resulting in 8-fold greater recovery of DNA across the variety of soil types.