QIAxcel RNA QC Kit v2.0

QIAxcelでRNAの定量/定性自動解析

S_1084_8_GEN_Kitrot

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QIAxcel RNA High Sensitivity Kit (1200)

Cat. No. / ID:  929112

QIAxcel RNA High Sensitivity Gel Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, QX RNA Alignment Marker, QX RNA Size Marker 200–6000 nt, QX RNA Denaturation Buffer, 12-Tube Strips
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¥225,000
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Cartridge type
High Sensitivity
RNA QC
Note: The QIAxcel High Sensitivity Kit only works with the QIAxcel Connect
QIAxcel RNA QC Kit v2.0は分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 即使用可能なゲルカートリッジで安全かつ簡便なシステム
  • 能率的なワークフローで最小限の解析時間
  • わずか5 ng/μlのトータルRNAから信頼できる結果
  • アガロースゲル電気泳動よりも高い感度
  • デジタルデータ収集によりプロセスの標準化

Product Details

QIAxcel Advanced System および QIAxcel SystemとQIAxcel RNA QC Kit v2.0を組み合わせると、1ランあたり最大96個のRNAサンプルまでの定性/定量解析を自動的に行ないます。サンプルアプライおよび解析の自動化は、サンプルの取り扱い時間を短縮し、RNA分解やコンタミのリスクを最小限に抑えます。本システムは、わずか5 ng/μl の希釈トータルRNA(オリジナルのRNA溶液では10 ng/µl)および50 ng/µlのcRNAあるいは一本鎖cDNAを検出できます。

Performance

高速かつ高感度なRNA解析

遺伝子発現研究やmiRNA研究においてはリアルタイムPCRやマイクロアレイ解析などの感度の高いテクノロジーが利用されています。これらの研究で信頼できる結果を得るためには、RNAが分解していないことが非常に重要な因子となります。分解度は検出結果に強く影響するので、スタートサンプルのトータルRNAの分解度や全プロセスで作製される産物の分解度をモニタリングすることは重要です。完全自動化されたQIAxcel Advanced System および QIAxcel SystemはトータルRNAおよび断片化/インタクトなcRNAの分解度および品質の高速かつ高感度な解析を実現します。サンプルの分解状態や完全性はQIAxcel Advanced System および QIAxcel Systemで容易に解析できます(図" 分解したRNAの解析")。サンプルアプライの自動化および解析は、サンプルのマニュアル操作を削減し、RNA分解やコンタミのリスクを最小限に抑えます(図 " 植物からのRNA解析")。

QIAxcel Advanced System および QIAxcel SystemでのRNA解析は再現性が高く、Agilent Bioanalyzerに匹敵する結果が得られます(表参照)。

再現性の高い結果

75℃での時間(分)

QIAxcel ラン 1
28S/18S 比
QIAxcel ラン 2
28S/18S 比

Agilent BioAnalyzer
28S/18S 比

0 1.59 1.53 1.7
2 1.42 1.55 1.6
4 1.31 1.45 1.6
7 1.35 1.36 1.5
13 1.16 1.18 1.2
20 1.09 1.07 1.3
30 0.68 0.72 1.1
40 0.58 0.53 0.8
52 0.64 0.61 0.5
70 0.34 0.38 0.3
分解したRNAの解析に使用したサンプルと同一サンプルから得られた値である。
See figures

Principle

QIAxcel Advanced System および QIAxcel Systemは、核酸の大きさにより迅速に核酸を分離するためにキャピラリーゲル電気泳動を用いています(図 " サンプル分離プロセス")。従来のアガロースゲル電気泳動とは異なり、充填済みゲルカートリッジのキャピラリー内で分離が行なわれます。サンプルは自動的に個々のキャピラリーにアプライされ、電圧がかかります。負に電化した核酸はキャピラリー内を正に電化した末端まで移動します。アガロースゲル電気泳動と同様に、分子量の低い分子は高い分子よりも早く移動します。分子がキャピラリーを移動する際に通過する検出器が、蛍光シグナルを検出・測定します。光電子倍増管により蛍光シグナルが電子データに変換され、これがコンピューターにトランスファーされ、QIAxcel ScreenGel Softwareにより処理されます。処理後、データはエレクトロフェログラムとゲルイメージの両方で表示されます。
See figures

Procedure

QIAxcel RNA QC Kit v2.0 は、RNAの分離および解析を簡略化し、ユーザーの安全性向上を実現します。ゲルカートリッジをセット、バッファートレイの充填とセット、96ウェルプレート/PCR チューブ/ストリップにサンプルをアプライ、使用するprocess profileを選択するといった、少ないステップの操作で簡単です。煩わしいゲル作製や専門的なトレーニングは不要なので、ラボのワークフローが能率化され、ルーチンワークへのインテグレーションが容易に行なえます。泳動開始後数分でコンピューター画面に最初の結果がリアルタイムで表示されます。

Applications

QIAxcel RNA QC Kit v2.0はトータルRNAおよび断片化あるいはインタクトなcRNAの分解度および品質を高速かつ高感度で解析します。

Supporting data and figures

Resources

パンフレット (3)
A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher
MSDS (1)
Download Safety Data Sheets for QIAGEN product components.
キットハンドブック (4)
QIAxcel システムを用いてRNAの定量/定性自動解析
For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments
For automated quantitative and qualitative RNA analysis using the QIAxcel Connect instrument
For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments
クイックスタートプロトコール (1)
機器ユーザーマニュアル (1)
For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6
Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (3)
A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher
Kit Handbooks (4)
For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments
QIAxcel システムを用いてRNAの定量/定性自動解析
For automated quantitative and qualitative RNA analysis using the QIAxcel Connect instrument
For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments
Quick-Start Protocols (1)
Instrument User Manuals (1)
For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6

FAQ

Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?

The intensity calibration procedure on the QIAxcel System and QIAxcel Advanced is performed to normalize the signal intensities across all 12 channels of the gel cartridge. This corrects for natural intensity reading variations between each capillary in the cartridge. A correction factor is determined and applied for every subsequent run performed with the cartridge.

FAQ ID -1824
What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing?

No, the RNA gel itself is not denaturing. It is a native polymer gel. 

FAQ ID - 3366
What is the concentration range for RNA Integrity Score analysis when using the QIAxcel RNA High Sensitivity Kit?
When analysing RNA Integrity Score with the QIAxcel RNA High Sensitivity Kit - samples must be in the concentration range of 1 ng/µL - 50 ng/µL. 
FAQ ID - 3984

Where are the QIAxcel data stored?

For the BioCalculator software, the default data folder is:

C:\Program Files\QIAGEN\QIAxcel BioCalculator\Data.

For the ScreenGel software, the default data folders are:

C:\Documents and Settings\All Users\Application Data\QIAGEN\QIAxcel\ScreenGel\Data\Experiment (for a computer with Windows XP operation system) or C:\ProgramData\QIAGEN\QIAxcel\ScreenGel\Data\Experiment\ (for a computer with Windows 7 operating system).

Alternatively, you can more conveniently access the ScreenGel experiment data folder using the menu options File\Open data directory\Experiment within the ScreenGel software.

FAQ -9008
How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?

If the gel cartridge tips for the QIAxcel System and QIAxcel Advanced are in contact with air, the tips will dry out. There are 3 ways of preventing the tips from drying.

  1. Store the cartridge in the instrument with the instrument in the “Park” position. The washing solution (8 ml) covered with mineral oil must be in the “Park” position of the Solution Tray. 
  2. Store the cartridge in the Cartridge Stand with enough mineral oil in the well of the Cartridge Stand to cover the capillary tips. The Cartridge Stand should only be used for short term storage, i.e. when changing cartridges during one working day. 
  3. Place the cartridge back into its packaging with the capillary tips gently inserted into the soft gel in the box.
FAQ ID -1823
Why does the ScreenGel software version 1.2 crash when I try to use an old reference size marker table?

The reference size marker table created by ScreenGel version 1.1 or earlier is incompatible with ScreenGel version 1.2. Re-analyze the size marker run in ScreenGel version 1.2 and create a new reference size marker table for future use.

FAQ -9009
Why is the cartridge not recognized?

When using the BioCalculator software, ensure the color of the software key matches the type of cartridge used. For example, the QIAxcel Fast Analysis cartridge requires the red software key, while the QIAxcel High Resolution cartridge requires the blue software key.

In addition, check that the communication between the computer and the instrument is established. Further, check that the smart key is inserted securely.

If the issue persists, contact QIAGEN Technical Services and provide the cartridge ID and lot number, which is printed on the packaging of QIAxcel cartridges.

FAQ -9004
How do I perform the filter test?

When using the BioCalculator software, select Settings under File in the Instrument control panel window. In the new windows, click Check Filter in the new window and follow the on-screen prompts.

When using the ScreenGel software, log in to the ScreenGel software with an account with basic or advanced privilege.  Select System Check under the Service environment. Select Filter Check and click Start.

Please send the filter test result to QIAGEN Technical Services for further assistance.

FAQ -9006
Why is the signal of the QX alignment marker weak?

Incorrect storage can lead to QX alignment marker degradation. The QX alignment markers can be used for 50 runs or 3 days, whichever comes first. Opened QX alignment marker should be store at –20°C between uses.

FAQ -9003
I have upgraded the ScreenGel software to version 1.2. How can I stop the software crash when I try to select a cartridge?

Please delete the 6 obsolete method files as outlines in the Important Note QIAxcel ScreenGel Software

FAQ -9010
How do I perform the detector test?

When using the BioCalculator software, select Detector test under File in the Instrument control panel window. Perform the Detector test with and without a cartridge latched inside the QIAxcel. Feedback is not shown on the screen when you select the Detect test. Instead, the file named detector.asc is saved at C:\Program Files\QIAGEN\QIAxcel BioCalculator. Change the file name before performing another detector test.

Note: Older versions of BioCalculator software save the detector.asc at C:\Program Files\QIAxcel BioCalculator.

When using the ScreenGel software, log in to the ScreenGel with an account with basic or advanced privilege.  Select System Check under the Service environment. Select Detector Test and click Start. The file is saved at C:\Documents and Settings\All Users\Application Data\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting\Troubleshooting_xxxxxxxx (current date in the format of YYYYMMDD) for a computer with Windows XP operating system or C:\ProgramData\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting for a computer with Windows 7 operating system. Alternatively, you can perform a screen capture for the Detector test result.

Please send the Detector test results to QIAGEN Technical Services for further assistance.

FAQ -9006