Microbial DNA-Free Water

Microbial DNA qPCR製品と使用

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Microbial DNA-Free Water

Cat. No. / ID: 338132

12 tubes of 1.35 ml each

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￥11,500

Microbial DNA-Free Waterは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Microbial DNA qPCR アッセイでの使用のために厳密な検証済み
0.22 μMフィルターで滅菌済み
コンタミしたゲノムDNAを破壊するためのUV処理済

Product Details

Microbial DNA-Free Waterは分子生物学グレードのヌクレアーゼフリー水で、ゲノムDNAのコンタミを除去するためにフィルター滅菌とUV処理がされています。品質はバクテリアおよび菌類DNAに反応するMicrobial DNA qPCR AssayのパネルでPCRにより検証済みです。Microbial DNA-Free Waterをテンプレートとして使用したPCRアッセイにおいてポジティブ反応が無い場合にロットが検査を通過します。この厳密なプロセスはMicrobial DNA qPCR Assay またはArray実験の結果を最も正しいものにさせることができます。

Performance

Microbial DNA-Free Waterの各ロットのクオリティコントロールで、微生物DNAが存在しないことが検証されています。クオリティコントロールテストの結果は、製品に同梱されるプロダクトシートに記載されます。

Principle

厳しい精製および検証プロセスは、Microbial DNA-Free Waterが清浄でゲノムDNAのコンタミが完全にないことを確実にします。このMicrobial DNA-Free Waterは0.22 μMフィルターで滅菌され、ゲノムDNAがUV処理で除去されています。この浄化工程が完了すると、各ロットのMicrobial DNA-Free Waterで、Microbial DNA qPCR Assayを用いて菌類およびバクテリアDNAのコンタミを確認するクオリティコンロトールテストが行なわれ、各アッセイでネガティブシグナルが得られた場合にのみテスト合格とみなされます。

Procedure

Microbial DNA-Free WaterはMicrobial DNA qPCR ArrayおよびAssayでの使用に最適で、濃縮されたMicrobial DNA Positive Control、とMicrobial qPCR Mastermixの希釈用、およびNo Template Controlとして使用できます。

Applications

Microbial DNA-Free Water is highly suited for use with Microbial DNA qPCR Arrays and Assays as a diluent for concentrated Microbial DNA Positive Control and Microbial qPCR Mastermix, and as a no-template control.

Resources

Download Safety Data Sheets for QIAGEN product components.

For application-specific microbial identification and profiling by real-time PCR

For real-time PCR-based profiling/detection of microbial species, antibiotic resistance genes or virulence factor genes

For application-specific microbial identification and profiling by real-time PCR

For real-time PCR-based profiling/detection of microbial species, antibiotic resistance genes or virulence factor genes

FAQ

The Microbial DNA qPCR Assays for bacterial species detection use the 16S rRNA gene as the template. Microbial DNA qPCR Assays for detection of fungal species use ribosomal (small subunit or larger subunit) genes as the template. Microbial DNA qPCR Assays for detection of microbial genes use various antibiotic resistance genes and virulence factor genes for the template.

FAQ ID — 3403

Store Microbial DNA qPCR Array plates at room temperature.
Store Microbial DNA qPCR Assays, Microbial DNA qPCR Assay Kits, and Microbial DNA qPCR Multi-Assay Kits at –20°C.
Store Microbial qPCR Mastermix at –20°C.
Store Microbial DNA Positive Control at –20°C.
Store Microbial DNA-Free Water at –20°C.

FAQ ID — 3394

Limit of detection (LOD) is defined as the lowest concentration at which 95% of positive samples are detected. Since LOD is not necessarily within the linear range of an assay, LOD can be lower than LLOQ.

FAQ ID — 3402

The Microbial DNA qPCR Arrays and Assay Kits do not allow measurement of antibiotic resistance gene expression because reverse transcriptase enzyme is not included. Microbial DNA qPCR Assays have not been tested on cDNA.

FAQ ID — 3404

The lower limit of quantification (LLOQ) is the lower limit at which an assay can provide quantitative results. It is the lowest template concentration that is within the linear range of the assay.

FAQ ID — 3401

Most of the Microbial DNA qPCR Assays that detect bacterial species are species-specific. Assays that do detect more than one species will list which species are detected in the product specification sheet. These were determined by both experimental data and in silico prediction.

FAQ ID — 3400

The Microbial qPCR Mastermix (ROX) is suitable for use with the following real-time cyclers: all Applied Biosystems and Agilent (formerly Stratagene); BioRad Opticon, Opticon 2, and Chromo 4; Roche LightCycler 480; and Eppendorf Mastercycler ep realplex 2, 2S, 4, and 4S.

The Microbial qPCR Mastermix (Fluorescein) is suitable for use with the following real-time cyclers: BioRad iCycler, MyiQ, and iQ5.

FAQ ID — 3395