TissueLyser LT

We do not sell ceramic beads or glass beads for use with our TissueLyser II or TissueLyser LT instruments. However, we still sell stainless steal beads and tungsten carbide beads. For more information, please go to this link.

TissueLyser LT Adapter is tolerant to temperatures between –78.5°C and +150°C.

Do not freeze the TissueLyser LT Adapter or the sample tubes in liquid nitrogen. The adapter and tubes may break and may cause injury.

However, the insert of the TissueLyser LT Adapter, the sample tubes, and grinding beads can be placed on dry ice for cooling prior to disruption.

Height: 280 mm

Width: 150 mm

Depth: 270 mm

Efficient DNA isolation requires thorough sample disruption and digestion.

Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used.

To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight. DNA yields may be improved by increasing the amount of Proteinase K or by adding additional proteinase K after several hours of digestion.  

We are sorry, but we do not offer repair on the TissueLyser LT.

Buffer ATL can produce foam when used in conjunction with the TissueRuptor. You can add 0.5% final volume of Reagent DX (cat. no. 19088) to prevent the formation of foam.

Any number of samples between 1 and 12 can be processed on the TissueLyser LT. If processing either 1 sample or 11 samples, load an additional, empty sample tube to balance the TissueLyser LT Adapter. For more information please see the TissueLyser LT Handbook.

See: TissueLyser Virtual Demo for a virtual demonstration on how the TissueLyser LT works.

We recommend using 2 ml Sample Tubes RB (cat. no. 990381) with our TissueLyser LT.

We do not guarantee the stainless steel beads to be RNase free. However, when used according to our specifications for disruption of biological samples, no special treatment is needed to make the beads RNase free. This is because our chaotropic lysis buffers will usually inactivate these and RNases contained in the samples themselves.

Our stainless steel beads and tungsten carbide beads are ready to use.

The TissueLyser LT has the following weight and dimensions:

• Weight: Approx. 7.35 kg (net); approx. 9 kg (gross)
• Width: 150 mm
• Depth: 270 mm
• Height: 280 mm

When disrupting tough or very tough samples, we recommend using one and two 7 mm stainless steel beads, respectively, instead of one 5 mm stainless steel bead, to guarantee optimal disruption.

Do not exceed the recommended amount of tissue per sample tube. Please refer to the TissueLyser LT Handbook for our recommendations of starting sample amount for purification of DNA, RNA, or proteins from various sample types.

You may need to increase the time and/or intensity of homogenization from what is recommended in the TissueLyser LT Handbook. But please be aware that this may lead to some fragmentation of gDNA.

Yes, if using fresh samples that are not stabilized with a stabilization reagent (e.g., RNAprotect Tissue Reagent or Allprotect Tissue Reagent) the TissueLyser LT Adapter can be precooled on dry ice. During the disruption process the adapter will passively cool the samples to prevent heating and degradation of nucleic acids or proteins.

See:  TissueLyser Virtual Demo for a virtual demonstration of this precooling process with the TissueLyser LT.

Yes, the TissueLyser LT Adapter can completely be removed from the instrument for cleaning. For more information on how to clean or decontaminate the instrument and the adapter please refer to chapter 6.1 in the TissueLyser LT User Manual on regular maintenance. The TissueLyser LT Adapter must not be autoclaved.

See:  TissueLyser Virtual Demo for a virtual demonstration on how the TissueLyser LT Adapter is used 

The largest and most efficient beads that can be used with the TissueLyser LT are the 7 mm stainless steel beads. We suggest pre-disrupting teeth or bone into smaller pieces (e.g., using a hammer) and then use the resulting pieces for further disruption in the TissueLyser LT. In this case, we suggest cooling the samples and the adapter on dry ice prior to disruption, rather than disruption in a lysis buffer.

Please note that the adaptor and tubes should not be cooled using liquid nitrogen, as this could lead to breakage of the TissueLyser Adapter and the sample tubes and may cause injury.

Yes, the 2 ml Sample Tubes RB (cat. no. 990381) can be used with both TissueLyser II and TissueLyser LT.

Stainless steel beads can be reused for DNA extractions with DNeasy Plant Kits. Used beads can be recovered from cell-debris and cleaned using the procedure below:

1. Close the cap of the 2 ml microtube and briefly vortex to dislodge the bead and pellet from the bottom of the tube. If using grinding jars for the disruption of large sample volumes, skip to step 2.
2. Empty the contents of the tubes/jars into a sieve and rinse the beads thoroughly with water.
3. Incubate beads in 0.4 M HCl for 1 min at room temperature (15–25°C) to degrade any DNA and avoid cross-contamination in future preparations.
4. Rinse beads thoroughly with distilled water to remove the HCl.
5. Dry beads before use.

You can also find these instructions in Appendix B of the DNeasy Plant Handbook.

In general, disruption works very well using one stainless steel bead with a diameter of 5 mm. However, for tougher samples we recommend the use of two 7mm stainless steel beads to optimize the effectiveness of disruption.

See:  TissueLyser Virtual Demo for a demonstration on how these beads are used with the TissueLyser LT.

We do not sell 3 mm stainless steel beads for use with our TissueLyser instruments.