ライゲーション計算ツール
一般的なライゲーション反応に適した範囲で、目的のインサート/ベクターモル比にするために必要なインサート量を計算します。
洞察
インサートDNA所要量 [g] = ベクターDNA量 [g] x {インサートDNA長/ベクターDNA長} x 目的のインサート/ベクターモル比
注：モル比は{1, 2, 3, 5 ,7}から選択します。
インサート長はベクター長よりも小さい値にします。
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推奨製品
CAT NO./ID 203123
AllTaq PCR Core Kit (250 U)
50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 1.9 ml RNase-Free Water
CAT NO./ID 201203
Taq DNA Polymerase (250 U)
250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
CAT NO./ID Y9140-LC-L
End-Repair Mix (low concentration)
For 200 low-concentration DNA reactions. End-Repair Mix 1X (0.20 mL), 10X End-Repair Buffer (1 x 1.5 mL) and 1 mM dNTP solution (0.5 mL).
CAT NO./ID 19092
Pathogen Lysis Tubes L
50 Pathogen Lysis Tubes with large beads, 1 vial of Reagent DX
CAT NO./ID 333777
QIAseq 96-Index L (384)
Box containing oligos for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Ion Torrent platforms; enough to process a total of 384 samples
CAT NO./ID 333764
QIAseq 12-Index L (48)
Box containing oligos for indexing up to 12 samples for QIAseq Targeted Panel sequencing on Ion Torrent platforms; enough to process a total of 48 samples
CAT NO./ID 333491
QIAseq Targeted DNA Pro Index L (12)
Box containing oligos for indexing up to 12 samples for QIAseq Targeted DNA Pro Panel sequencing on Ion Torrent platforms
CAT NO./ID 9018093
Cooling Adapter, LC capillaries 32, Qsym
Adapter for holding up to 32 capillaries (20 µl) for the LightCycler; for use with the QIAsymphony AS (software version 3.1 or higher)
CAT NO./ID 333127
QIAseq Targeted RNA 96-Index HT l (384)
Kit containing sample indexes, enough for a total of 384 samples, for indexing up to 96 samples for targeted RNA sequencing on Illumina platforms (array format), and primers necessary for sequencing RNA libraries generated by the QIAseq targeted RNA panels on Illumina platforms
CAT NO./ID 4501063
artus EBV LC PCR Kit (24) CE
For 24 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
CAT NO./ID 4502063
artus VZV LC PCR Kit (24) CE
For 24 reactions on the LightCycler 1.1/1.2/1.5/2.0 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
CAT NO./ID L6030-HC-L
T4 DNA Ligase (Rapid)
240,000 U of T4 DNA Ligase (600,000 U/mL) 2x Rapid Ligation Buffer and 10x T4 DNA Ligase Buffer
CAT NO./ID 19092
Pathogen Lysis Tubes L
50 Pathogen Lysis Tubes with large beads, 1 vial of Reagent DX
CAT NO./ID 334942
QIAseq Multimodal HC Panel HT (12)
QIAseq Multimodal HC Panel HT (12) contains ALL reagents (except indices) sufficient to process 12 samples for multimodal HT (DNA and RNA) sequencing; fixed high content panel for a total of 24 reactions
CAT NO./ID 333777
QIAseq 96-Index L (384)
Box containing oligos for indexing up to 96 samples for QIAseq Targeted Panel sequencing on Ion Torrent platforms; enough to process a total of 384 samples
CAT NO./ID 333764
QIAseq 12-Index L (48)
Box containing oligos for indexing up to 12 samples for QIAseq Targeted Panel sequencing on Ion Torrent platforms; enough to process a total of 48 samples
CAT NO./ID 333512
QIAseq Targeted DNA HC Panel (12)
Kit containing ALL reagents (except indexes) for targeted DNA sequencing; fixed panel for 12 samples; more than 100 genes
CAT NO./ID 333661
QIAseq Targeted DNA Pro HC (12)
Kit containing all reagents (except indices) for targeted DNA sequencing; fixed panel for 12 samples
CAT NO./ID 333612
QIAseq Targeted RNAscan HC Panel (12)
Kit containing ALL reagents (except indexes) for targeted Fusion sequencing; fixed panel for 12 samples; more than 100 fusions
CAT NO./ID 333615
QIAseq Targeted RNAscan HC Panel (96)
Kit containing ALL reagents (except indexes) for targeted Fusion sequencing; fixed panel for 96 samples; more than 100 fusions
CAT NO./ID L6020L
T7 DNA Ligase (900,000 U)
900,000 U of T7 DNA Ligase (3,000,000 U/mL) and 2x Rapid Ligation Buffer
CAT NO./ID P7060L
Klenow Fragment (2500 U)
2500 U of Klenow Fragment (0.5 mL at 5000 U/mL) and 10x Blue Buffer (1 x 1.5 mL)
CAT NO./ID P7250L
Taq-B DNA Polymerase (10,000 U)
10,000 U of Taq-B Polymerase (2 mL at 5000 U/mL) and 10x PCR Buffer.
CAT NO./ID RP1010
TaqNova Polymerase UCP (1000 U)
Ultra-Clean Production TaqNova DNA Polymerase.
5 U/µL concentration with reaction buffer of 10x TaqNova DNA-free Reaction Buffer, and 50 mM MgCl2Storage buffer: 20 mM Tris-HCl (pH 8.0 at 25°C); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5% (v/v) Nonident P40; 0.5% (v/v) Tween 20; 50% (v/v) glycerol
Storage temperature: -20°C
CAT NO./ID 19101
RNase A (17,500 U)
2.5 ml (100 mg/ml; 7000 units/ml, solution)
Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.
CAT NO./ID 203203
HotStarTaq DNA Polymerase (250 U)
250 units HotStarTaq DNA Polymerase, 10x PCR Buffer, 5x Q-Solution, 25 mM MgCl2
CAT NO./ID 205111
Omniscript RT Kit (50)
For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1.1 ml RNase-free water
CAT NO./ID 79254
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps