Automate the purification of high-quality gDNA from human whole blood samples (up to 4 ml) using the QIAsymphony SP.
Automated DNA purification from 4 ml on the QIAsymphony SP results in high yields (measured via gel electrophoresis and spectrophotometer) of high-molecular-weight DNA (see High-molecular-weight DNA) in 4.75 hours (see QIAsymphony DNA Maxi Kit workflow), and even faster for 2 ml or for fewer than 24 samples.
The QIAsymphony DNA Maxi Kit uses an interlaced system (see QIAsymphony DNA Maxi Kit workflow) that shortens the time needed to complete the process:
The QIAsymphony SP performs all 4 steps (lyse, bind, wash and elute) of the purification procedure for up to 36 samples in a single run.
|Applications||PCR, real-time PCR, genotyping|
|Elution volume||200–500 µl|
|Main sample type||Human whole blood|
|Sample amount||2 or 4 ml (smaller volumes can be adjusted with PBS to 2 or 4 ml)|
|Yield||60–150 µg from 4 ml sample (on average; samples with particularly high or low WBC count may fall outside that range)|