Types of PCR
Methylation-specific PCR (MSP)
Hot start PCR
RAPD: Rapid amplified polymorphic DNA analysis
RACE: Rapid amplification of cDNA ends
In situ PCR
Differential display PCR
Differential display PCR is based on RT-PCR and is used to compare and identify differences in mRNA (and therefore gene) expression patterns between two cell lines or populations.
In this technique, first-strand cDNA synthesis is primed with an anchored primer complementary to ~13 nucleotides of the poly(A) tail of mRNA and the adjacent 2 nucleotides of the transcribed sequence. After reverse transcription and amplification, amplified products are visualized using gel electrophoresis. The banding patterns observed can be compared to identify differentially expressed cDNAs in the 2 populations.
Invented in the 1990s, the technique fast became a key tool in gene expression analysis. However, it has been more recently superseded by RNA-seq, microarrays, and qRT-PCR.