QIAsymphony SP/AS Accessories

For use with the QIAsymphony SP/AS instruments

Features

Useful Resources

Important Note — Sample Prep Cartridge positioning in unit boxes Using the EGFR PCR Kit on the Rotor-Gene® Q Instrument - (EN) (EN) - QIAsymphony RGQ — Pure Performance

Carrier, tubes (1), sample track, Qsym

Cat. No. / ID: 9019873

Tube carrier for sample input; for use with the QIAsymphony SP

$518.00

ProductTubeAccessoriesTips

Carrier, tubes (1), sample track, Qsym

Adapter 2 x Rotor-Disc, Qsym

Accessory Trough

Tube Insert, 11 mm, Revision, sample carrier, Qsym (24)

Tubes, conical,

Tube Insert, 13 mm, sample carrier, Qsym (24)

Tubes, conical

Cooling Adapter, PCR, v2, Qsym

Adapter, tubes, 2 ml, v2, Qsym

Cooling Adapter, EMT, v2, Qsym

Insert, 2.0ml v2, samplecarr. (24), Qsym

Cooling Adapter, MTP, RB, v2, Qsym

Cooling Adapter, Reagent Holder 1, Qsym

Cooling Adapter, LC capillaries 32, Qsym

Cooling Adapter, Reagent Holder 2, Qsym

Cooling Adapter, RG Strip Tubes 72, Qsym

Cooling Adapter, 2 ml, v2, Qsym

Cooling Adapter

8-Rod Covers

Cooling Adapter, Snap-Cap Microtube QIAsymphony, Qsym

Filter-Tips

Rotor-Disc 72 Loading Block

Reagent Cartridge Holder

Reuse Seal Set

Set, O-Ring, Qsym

Tip Disposal Bags (15)

Sample Carrier, plate, Qsym

Sample Prep Cartridges

Quantity

Add to cart

Product Details

QIAsymphony SP/AS Accessories are for use with the QIAsymphony SP/AS instruments for automated sample preparation and assay setup. When ordering adapters, ensure that you order the correct adapter for the software version that you are using.

Resources

Important information regarding proper positioning of Sample Prep Cartridges within unit boxes

FAQ

No. The master mixes in Rotor-Gene Kits contain dTTP instead of dUTP. If UNG treatment is required, we recommend using QuantiTect +UNG Kits. QuantiTect Kits are also compatible with the Rotor-Gene Q; however, the kits require a significantly longer cycling time.

FAQ ID -2117

Yes. The optimized master mix of the Rotor-Gene Multiplex PCR Kit ensures that all gene targets in a multiplex reaction are amplified with the same efficiency and sensitivity as in corresponding singleplex reactions, independent of starting copy numbers.

FAQ ID -2129

Yes. Samples have to be completely lysed and homogenized prior to automated processing on the QIAsymphony SP platform using the QIAsymphony DSP DNA Mini Kit. Tissue samples are lysed by proteinase K digestion. FFPE tissue samples are deparaffinized with Deparaffinization Solution or xylene/ethanol and lysed by proteinase K digestion. Insoluble material is pelleted by centrifugation. Lysates are placed on the QIAsymphony SP for purification of DNA (see protocol sheets for details).

FAQ ID -2926

The Rotor-Gene Multiplex PCR Kit has been developed primarily for use with TaqMan® probes. Protocols for probes other than TaqMan® probes have not been tested.

See trademarks.

FAQ ID -2130

We strongly recommend use of the dedicated Rotor-Gene Q Accessories, e.g., PCR Tubes 0.2 ml or Strip Tubes and Caps 0.1 ml provided by QIAGEN. These tubes are designed to give low background and perfectly match the Rotor-Gene Q requirements.

FAQ ID -2085

The Rotor-Gene Q instrument can be used with two different rotor formats: using tubes or rotor-dics. Tubes can be run in 36 and 72-well rotors and rotor-discs in Rotor-Disc 72 and Rotor-Disc 100 rotors. When programming the temperature profile please make sure the correct rotor type is selected.

FAQ ID -1519

There are several manufacturers of high-quality real-time cyclers. These include QIAGEN's Rotor-Gene Q, Applied Biosystems, BioRad, Stratagene, Eppendorf, Roche, TaKaRa, Fluidigm, and Cepheid. The important thing to keep in mind is that, once you select an instrument to use, you must use compatible Rotor-Gene Discs and tubes, 96 or 384 well plates, and qPCR master mixes that are optimized for use in that particular instrument. For example, QIAGEN's Rotor-Gene SYBR Green, Probe, and Multiplex real-time master mixes.

FAQ ID -2670

The majority of the Rotor-Gene Kit data shown in our literature has been generated with the help of the QIAgility instrument. We did not observe any problems during the pipetting steps.

FAQ ID -2116

Rotor-Gene Kits are specifically developed for the Rotor-Gene Q PCR Cycler. The unique rotary system of the cycler combined with the kits’ proprietary buffer system enable ultrafast cycling. Rotor-Gene Kits do not contain ROX dye since no normalization to a passive reference is required. Also, Rotor-Gene Kits do not contain dUTP; therefore, UNG pretreatment is not possible.

FAQ ID -2119

The Globally Harmonized System (GHS) is an initiative by the United Nations aiming to harmonize chemical hazard communication worldwide. Hazards symbols accompanying products are changing to those of the GHS. Information on the adoption of this new system is available on our GHS Hazard Labeling pages.

FAQ ID - 3430