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DNeasy Plant Mini Kit

For extraction of up to 30 µg total cellular DNA from plant cells and tissues, or fungi
  • Pure DNA, free from contaminants and enzyme inhibitors
  • Rapid extraction of ready-to-use DNA
  • No organic extraction, no ethanol precipitation

The DNeasy Plant Mini Kit provides fast and easy silica-based plant DNA extraction in convenient spin column format. Typical yields are 3–30 μg of high-quality DNA, depending on the samples used (e.g., wheat, maize, arabidopsis, tomato, tobacco). Extraction of plant DNA using the DNeasy Plant Mini Kit can be automated on the QIAcube Connect

DNeasy Plant Pro Kit – superior DNA yields and inhibitor removal from plant samples - See new kit.

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Cat No./ID: 69104
DNeasy Plant Mini Kit (50)
$236.00
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50 DNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)
Cat No./ID: 69106
DNeasy Plant Mini Kit (250)
$1,052.00
Order Product
250 DNeasy Mini Spin Columns, 250 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)
The DNeasy Plant Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

0
DNeasy Plant and DNeasy 96 Plant procedures.
1
PCR analysis.
DNA (10 ng) from the indicated leaves or needles was amplified using universal primers for the noncoding intergenic spacer between the tRNA genes trnL (UAA) 5' exon and trnL (UAA) 3' exon of cpDNA (Taberlet, P. et al. (1991) Plant Mol. Biol. 17, 1105). M: 100 bp ladder.
2
RAPD analysis.
DNA (50 ng) from leaves of the indicated in vitro propagated sunflower (Helianthus) species was amplified using a 10-base RAPD primer and separated on a 1.5% agarose gel. M: 100 bp ladder. (Data kindly provided by H. J. Henn, Institute of Agricultural Botany, University of Bonn, Germany.)
3
Pure DNA (20–25 kb) for restriction analysis.
Agarose gel (0.8%) analysis of DNA isolated from the indicated leaves or needles with the DNeasy Plant Mini Kit was perfromed. Undigested DNA (0.5 µg, left) or 1 µg of EcoRI-digested DNA (right) were loaded in each pair of lanes. M: lambda-HindIII.
4
DNA purity from oak leaves and pine needles.
Spectrophotometric scans (220–320 nm) of DNA isolated from pine needles using the method of Dellaporta, CTAB, or the DNeasy Plant Mini Kit. Pure DNA typically shows a symmetrical peak at 260 nm and a smooth profile. Polysaccharides and other secondary metabolites, often copurified with plant DNA isolated using traditional methods, can interfere with OD readings (A260/ A280), leading to errors in determination of concentration and purity.
Performance

The DNeasy Plant Mini Kit allows rapid and efficient isolation of high-quality DNA from a wide variety of plant species and tissue types including the most demanding sources (see table "Selection of plant species processed with DNeasy Kits"). Samples may be fresh, frozen, or dried. The optimized DNeasy Plant procedure incorporates the QIAshredder spin column, a unique filtration and homogenization column that efficiently removes cell debris and improves sample handling following lysis.

Selection of plant species processed with DNeasy Kits 
Abies alba (silver fir) Nicotiana tabacum (tobacco)
Aesculus hippocastanum (horse chestnut) Oryza sativa (rice)4
Arabidopsis thaliana (thale cress) Pelargonium sp. (geranium)4
Avena sp. (oat) Petunia sp.4
Brassica napus (oilseed rape) Pinus sylvestris (Scotch pine), P. brutia5
Brassica oleracea (kohlrabi) Populus tremula (aspen)
Chicorium endivia (chicory) Pseudotsuga menziesii (Douglas fir)
Citrullini lanatus (water melon) Quercus robur, Q. petrea (oak)6,7
Egeria sp. Rhododendron sp.2,4
Fagus sylvatica (beech)1 Rubus idaeus (raspberry)
Helianthus spp. (sunflower) Solanum tuberosum (potato)
Hordeum vulgare (barley)2 Sphagnum palustre (moss)
Humulus sp. (hops) Spinacia oleracea (spinach)
Hydrilla sp. Taxus baccata (yew)
Kalanchoe spp. Triticum aestivum (wheat)4
Lupinus sp. Ulmus glabra (elm)6
Lycopersicon esculentum (tomato)3 Vitis spp. (grape)6
Myriophyllum sp. Zea mays (maize)
Young leaves or needles (and other tissues, as indicated) were collected and immediately flash frozen. DNA isolation was then performed with the DNeasy Plant Mini Kit. 1Beechnut, 2dried leaves, 3callus, 4leaves from adult plant, 5endosperm, 6old leaves, rich in carbohydrates, 7buds. For more information on DNA isolation from other species including fungi, call QIAGEN Technical Services or your local distributor.

The typical yield is 3–30 µg, with a sample size of up to 100 mg wet weight, and an elution volume of 50–400 µl. The DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and age of tissue sample. Lower and higher range values correspond to arabidopsis and wheat, respectively.

The DNeasy Plant procedure yields pure nucleic acid, free of polysaccharides and other secondary metabolites often copurified using conventional methods. Such impurities can interfere with spectrophotometric readings and inhibit enzymatic reactions. Absorbance scans of DNeasy purified DNA show a symmetrical peak at 260 nm (see figures "DNA purity from oak leaves and pine needles"), confirming that the DNA is free of impurities, including enzyme inhibitors. DNeasy purified DNA is sized up to 40 kb (see figure "Pure DNA (20–25 kb) for restriction analysis"). Purified DNA can be used in a wide range of applications (see figures "PCR analysis" and "RAPD analysis").

Principle

DNeasy Plant Kits use advanced silica-membrane technology and simple spin procedures to isolate highly pure total cellular DNA from plant tissues and cells or fungi. DNeasy technology replaces cumbersome DNA isolation procedures such as cetyltrimethylammonium bromide (CTAB), phenol, or chloroform extraction. Using the DNeasy procedure, alcohol precipitation is not necessary – purified DNA is ready for immediate use. DNeasy sample preparation technology is fully licensed.

Procedure

Samples are first mechanically disrupted and then chemically lysed (see flowchart "DNeasy Plant and DNeasy 96 Plant procedures"). RNA is removed by RNAse digestion during lysis. Cell debries, precipitated proteins, and polysaccharides are removed and the sample is homogenized by centrifugation through a QIAshredder spin column. Buffering conditions are adjusted and the lysate is loaded onto the DNEasy Plant Mini spin column. During a brief spin, DNA selectively binds to the silica membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use.

Applications

The DNeasy Plant Mini Kit provides purification of DNA from plant tissue, including:

  • Plant cells
  • Plant tissues
  • Fungi
  • Specifications

    Features
    Specifications
    Applications PCR, real-time PCR, blotting
    Elution volume 50–400 µl
    Format Spin column
    Main sample type Plant samples
    Processing Manual
    Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
    Sample amount <100 mg
    Technology Silica technology
    Time per run or per prep <1 hour
    Yield 3–30 µg

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