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SARS-CoV-2 N1+N2 Assay Kit

Primers and probes for real-time RT-PCR detection of SARS-CoV-2 for epidemiological research.
  • Primers based on the CDC primer design targeting N1 and N2
  • Probes coupled with FAM as a reporter dye for co-detection of both targets in the same channel
  • Primer and probe mixture purified by HPLC at a 20x concentration

The SARS-CoV-2 N1+N2 Assay Kit is compatible with the QIAprep&amp Viral RNA UM Kit.

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Cat No./ID: 222015
SARS-CoV-2 N1+N2 Assay Kit (600)
$200.00
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For 600 x 20 µl reactions: 1x 600 µl SARS-CoV-2 N1+N2 assay, 20x concentrate

Cat No./ID: 222017
SARS-CoV-2 N1+N2 Assay Kit (2400)
$720.00
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For 2400 x 20 µl reactions: 4x 600 µl SARS-CoV-2 N1+N2 assay, 20x concentrate

The SARS-CoV-2 N1+N2 Assay Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.


Product Details

Principle

The SARS-CoV-2 N1+N2 Assay is a mixture of four primers and two probes purified by HPLC at a 20x concentration. Therefore, only 1 µl of the assay is needed per 20 µl reaction.

The four primers are based on the CDC design, targeting the regions N1 and N2 of the viral genome. The two probes are coupled with FAM as a reporter dye and use ZEN™ quenchers for enhanced sensitivity (see table).

The SARS-CoV-2 N1+N2 Assay Kit does not include positive control.

Please ensure to follow the reaction setup and cycling condition provided by the suppliers of your RT-PCR chemistries and PCR cyclers to achieve the best performance.

Primer Concentration in 20x [µM] Concentration per reaction [µM] Sequence (5’-3’)
2019-nCoV_N1-F 16 0.8 GAC CCC AAA ATC AGC GAA AT
2019-nCoV_N1-R  16 0.8 TCT GGT TAC TGC CAG TTG AAT CTG
2019-nCoV_N2-F 16 0.8 TTA CAA ACA TTG GCC GCA AA
2019-nCoV_N2-R 16 0.8 GCG CGA CAT TCC GAA GAA
2019-nCoV_N1-P 5 0.25 FAM-ACC CCG CAT /ZEN/ TAC GTT TGG TGG ACC-3IABkFQ
2019-nCoV_N2-P 5 0.25 FAM-ACA ATT TGC /ZEN/ CCC CAG CGC TTC AG-3IABkFQ

 

Applications

The SARS-CoV-2 N1+N2 Assay Kit is intended for molecular biology applications such as epidemiological research using real-time RT-PCR.

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