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QIAseq FastSelect –5S/16S/23S Kits

For rapid 5S/16S/23S rRNA removal for RNA-seq library preparation from bacterial RNA samples
  • Pan-bacterial 5S/16S/23S rRNA removal from fragmented or full-length RNA for metatranscriptomics studies
  • Novel RNA removal mechanism that doesn't involve hybrid capture or enzymatic digestion
  • Combine with QIAseq FastSelect –rRNA HMR for removal of human, mouse, rat rRNA for host-microbe studies
  • Compatible with QIAGEN, Illumina, NEB and KAPA stranded RNA-seq library kits
  • In-silico design predicts blocking of >95% of all 5S, 16S and 23S rRNA database sequences

QIAseq FastSelect –5S/16S/23S Kits use a novel method to remove highly abundant bacterial 5S/16S/23S rRNA from your RNA-seq library. QIAseq FastSelect –5S/16S/23S was specifically designed for complex community microbial samples from soil, water, stool and sludge, which have numerous and diverse bacterial populations. The rRNA coverage provided by this kit is based on SILVA 16S sequences (nearly 600,000 entries), SILVA 23S sequences (nearly 170,000 entries) and 5S rRNA database sequences (over 7200 entries), and in-silico modeling shows coverage of >95% of all known 5S, 16S and 23S rRNA sequences.

QIAseq FastSelect –5S/16S/23S has been tested with QIAGEN, Illumina and NEB RNA-seq library kits and is compatible with all major methods of RNA-seq library preparation. The simple 14-minute protocol and bead cleanup integrates seamlessly with all common RNA-seq library kits, and can be used on fragmented or full-length (non-fragmented) RNA.

For removal of human, mouse, rat and other mammalian rRNA, please see our QIAseq FastSelect –rRNA HMR Kits.

Also, check out our QIAseq Stranded RNA Library Kits for robust RNA-seq library preparation.

Interested in customization of a QIAseq FastSelect RNA Removal Kit for your samples? Contact us!

Want to try this solution for the first time? Request a trial kit to evaluate.

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Cat No./ID: 335925
QIAseq FastSelect –5S/16S/23S Kit (24)
$1,338.00
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Includes 3 tubes of QIAseq FastSelect –5S/16S/23S rRNA removal reagent, reaction buffer, QIAseq Beads with QIAseq bead binding buffer and nuclease-free water sufficient for 24 samples
Cat No./ID: 335927
QIAseq FastSelect –5S/16S/23S Kit (96)
$4,634.00
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Includes 1 tube of QIAseq FastSelect –5S/16S/23S rRNA removal reagent, reaction buffer, QIAseq Beads with QIAseq bead binding buffer and nuclease-free water sufficient for 96 samples
Cat No./ID: 335929
QIAseq FastSelect –5S/16S/23S Kit (384)
$16,479.00
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Includes 4 tubes of QIAseq FastSelect –5S/16S/23S rRNA removal reagent, reaction buffer, QIAseq Beads with QIAseq bead binding buffer and nuclease-free water sufficient for 384 samples
QIAseq FastSelect –5S/16S/23S Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

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QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene expression
Total RNA was isolated from Gut Microbiome Whole cell Mix (ATCC) using the RNeasy PowerMicrobiome Kit (QIAGEN). Stranded transcriptome libraries were then prepared from 1 µg and 100 ng aliquots of the RNA using the QIAseq Stranded Total RNA Lib Kit, Illumina TruSeq Stranded Total RNA Library Prep Kit and NEBNExt Ultra II Directional RNA Library Prep Kit. For QIAseq FastSelect depletion, QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps, regardless of library prep kit, were completed (starting with 1st strand synthesis). Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

When rRNA depletion is performed using QIAseq FastSelect –5S/16S/23S, RNA expression results are highly reproducible, regardless of RNA input or RNA-seq library construction kit. (Log2, FPKM >0).
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QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene detection
Total RNA was isolated from Gut Microbiome Whole cell Mix (ATCC) using the RNeasy PowerMicrobiome Kit (QIAGEN). Stranded transcriptome libraries were then prepared from 1 µg and 100 ng aliquots of the RNA using the QIAseq Stranded Total RNA Lib Kit, Illumina TruSeq Stranded Total RNA Library Prep Kit and NEBNExt Ultra II Directional RNA Library Prep Kit. For QIAseq FastSelect depletion, QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps, regardless of library prep kit, were completed (starting with 1st strand synthesis). Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

As QIAseq FastSelect –5S/16S/23S substantially increases read budget, the average number of genes detected (FPKM >0) is robustly high, regardless of stranded RNA library prep kit and input amount.
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QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB
Total RNA was isolated from Gut Microbiome Whole cell Mix (ATCC) using the RNeasy PowerMicrobiome Kit (QIAGEN). Stranded transcriptome libraries were then prepared from 1 µg and 100 ng aliquots of the RNA using the QIAseq Stranded Total RNA Lib Kit, Illumina TruSeq Stranded Total RNA Library Prep Kit and NEBNExt Ultra II Directional RNA Library Prep Kit. For QIAseq FastSelect depletion, QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps, regardless of library prep kit, were completed (starting with 1st strand synthesis). Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

QIAseq FastSelect –5S/16S/23S results in highly efficient removal of rRNA, regardless of RNA input and regardless of stranded RNA library prep kit. The percentages of reads mapped to rRNA and rRNA removed are shown for both 1 µg and 100 ng aliquots of RNA for QIAseq Stranded Total RNA Lib Kit, TruSeq Stranded Total RNA Library Prep Kit, and NEBNext Ultra II Directional RNA Library Prep Kit.
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QIAseq FastSelect –5S/16S/23S vs. other methods: total reads mapped to rRNA
Total RNA was isolated from Gut Microbiome Whole cell Mix (ATCC) using the RNeasy PowerMicrobiome Kit (QIAGEN). Stranded transcriptome libraries were then prepared from 1 µg aliquots of the RNA using the QIAseq Stranded Total RNA Lib Kit. For rRNA depletion, three methods were used: Supplier TF – method ME and Supplier TF – method RM, which used a hybrid capture workflow, and Supplier QIAGEN – method QIAseq FastSelect –5S/16S/23S. For QIAseq FastSelect –5S/16S/23S, the QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps were completed (starting with 1st strand synthesis). For rRNA depletion using method ME and method RM, the workflows were followed according to the manufacture’s recommendations. Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

For method ME, method RM, and QIAseq FastSelect –5S/16S/23S, the percentage of total reads mapped to rRNA is shown for “all bacteria”, as well as individual species. QIAseq FastSelect –5S/16S/23S efficiently removes rRNA from a broad range of bacterial species, delivering superior performance compared to methods ME and RM.
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QIAseq FastSelect –5S/16S/23S workflow
Simply add QIAseq FastSelect reagent to the RNA sample, perform fragmentation (if required), stepwise cool the reaction from 75°C to 25°C over 14 minutes, perform a bead-based cleanup step and complete the remaining library prep steps. QIAseq FastSelect –5S/16S/23S has been tested with the QIAseq Stranded Total RNA Lib Kit (QIAGEN), TruSeq Stranded (Illumina), NEBNext Ultra II Directional (New England Biolabs) and KAPA RNA HyperPrep (KAPA Biosystems) library preparation kits. FastSelect is also compatible with most RNA library prep kits.

* Absolute requirement for fragmentation, as well as the duration of the fragmentation, depends on the library prep kit and sample type. Fragmentation is not required for QIAseq FastSelect –5S/16S/23S performance.
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QIAseq FastSelect –5S/16S/23S: robust rRNA removal from gut community sample
Total RNA was isolated from Gut Microbiome Whole cell Mix (ATCC) using the RNeasy PowerMicrobiome Kit (QIAGEN). Stranded transcriptome libraries were then prepared from 100 ng aliquots of the RNA using the QIAseq Stranded Total RNA Lib Kit (QIAGEN). For QIAseq FastSelect depletion, QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps were completed (starting with 1st strand synthesis). Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

A QIAseq FastSelect –5S/16S/23S results in highly efficient removal of rRNA. The percentage of reads mapped to rRNA is shown for both untreated and QIAseq FastSelect-treated samples, with QIAseq FastSelect removing nearly 87% of all rRNA. B The percentage of total reads mapped to rRNA is shown for “all bacteria”, as well as individual species. QIAseq FastSelect –5S/16S/23S efficiently removes rRNA from a broad range of bacterial species.
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QIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community (28 strains)
Total RNA was isolated from 20 Strain Even Mix Whole Cell Material (ATCC), Skin Microbiome Whole Cell Mix (ATCC) and Oral Microbiome Whole Cell Mix (ATCC) using the RNeasy PowerMicrobiome Kit (QIAGEN) and combined into a single sample (28 bacterial strains). Afterward, stranded transcriptome libraries were prepared from 100 ng aliquots using the QIAseq Stranded Total RNA Lib Kit. To remove bacterial rRNA, QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps were completed (starting with 1st strand synthesis). Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

A QIAseq FastSelect –5S/16S/23S results in highly efficient removal of bacterial rRNA. The percentage of reads mapped to rRNA is shown for both untreated and QIAseq FastSelect-treated samples, with QIAseq FastSelect removing over 80% of all rRNA. B By efficiently removing rRNA, QIAseq FastSelect–5S/16S/23S frees up substantial read budget, with more than 80% of the sequencing reads being available for gene expression analysis. In comparison, approximately 5% of the sequencing reads are available for gene expression analysis without rRNA removal treatment. C As QIAseq FastSelect –5S/16S/23S substantially increases read budget, the average genes detected (FPKM >0) increased 76% (from approximately 28,000 to nearly 50,000), demonstrating the value of robust rRNA removal.
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QIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community: mapping analysis (28 strains)
Total RNA was isolated from 20 Strain Even Mix Whole Cell Material (ATCC), Skin Microbiome Whole Cell Mix (ATCC) and Oral Microbiome Whole Cell Mix (ATCC) using the RNeasy PowerMicrobiome Kit (QIAGEN) and combined into a single sample (28 bacterial strains). Afterward, stranded transcriptome libraries were prepared from 100 ng aliquots using the QIAseq Stranded Total RNA Lib Kit. To remove bacterial rRNA, QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps were completed (starting with 1st strand synthesis). Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

The percentage of total reads mapped to rRNA is shown for “all bacteria”, as well as individual species. QIAseq FastSelect –5S/16S/23S efficiently removes rRNA from a broad range of bacterial species.
9
QIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiome
Total RNA was isolated from Gut Microbiome Whole cell Mix (ATCC) using the RNeasy PowerMicrobiome Kit (QIAGEN). Stranded transcriptome libraries were then prepared from 100 ng aliquots of the RNA using the QIAseq Stranded Total RNA Lib Kit. For FastSelect depletion, QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps were completed (starting with 1st strand synthesis). Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

A QIAseq FastSelect –5S/16S/23S results in highly efficient and specific removal of bacterial rRNA. The percentage of reads mapped to rRNA is shown for both untreated and QIAseq FastSelect-treated samples, with QIAseq FastSelect removing more than 85% of all rRNA. B By efficiently removing rRNA, QIAseq FastSelect –5S/16S/23S frees up substantial read budget, with more than 85% of the sequencing reads being available for gene expression analysis. In comparison, approximately 5% of the sequencing reads are available for gene expression analysis without rRNA removal treatment. C As QIAseq FastSelect –5S/16S/23S substantially increases read budget, the average genes detected (FPKM >0) increased 50% from approximately 20,000 to over 30,000, demonstrating the value of robust rRNA removal.
9
QIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiome: gene expression results
Total RNA was isolated from Gut Microbiome Whole cell Mix (ATCC) using the RNeasy PowerMicrobiome Kit (QIAGEN). Stranded transcriptome libraries were then prepared from 100 ng aliquots of the RNA using the QIAseq Stranded Total RNA Lib Kit. For QIAseq FastSelect depletion, QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps were completed (starting with 1st strand synthesis). Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

A Gene expression results are similar between samples with (QIAseq FastSelect –5S/16S/23S) and without rRNA removal (No treatment), suggesting that QIAseq FastSelect –5S/16S/23S exhibits high specificity for rRNA (Log2, FPKM >0). B Reproducibility in gene expression data between replicate QIAseq FastSelect –5S/16S/23S preparations is very consistent (Log2, FPKM >0, R2=0.98).
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Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S
Stranded transcriptome libraries were prepared from 1 µg and 100 ng aliquots of DH5ɑ E. coli total RNA (Thermo Fisher Scientific) using the QIAseq Stranded Total RNA Lib Kit. For QIAseq FastSelect depletion, QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps were completed (starting with 1st strand synthesis). Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

QIAseq FastSelect –5S/16S/23S results in highly efficient removal of rRNA, regardless of RNA input. The percentage of reads mapped to rRNA is shown for both 1 µg and 100 ng aliquots of E. coli RNA.
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Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression results
Stranded transcriptome libraries were prepared from 1 µg and 100 ng aliquots of DH5ɑ E. coli total RNA (Thermo Fisher Scientific) using the QIAseq Stranded Total RNA Lib Kit. For QIAseq FastSelect depletion, QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps were completed (starting with 1st strand synthesis). Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

When rRNA depletion is performed using QIAseq FastSelect –5S/16S/23S, gene expression results are highly reproducible, regardless of RNA input (A = 1 µg and B = 100 ng), (Log2, FPKM >0).
9
Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression results (100 ng, 1 µg)
Stranded transcriptome libraries were prepared from 1 µg and 100 ng aliquots of DH5ɑ E. coli total RNA (Thermo Fisher Scientific) using the QIAseq Stranded Total RNA Lib Kit. For QIAseq FastSelect depletion, QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps were completed (starting with 1st strand synthesis). Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

When rRNA depletion is performed using QIAseq FastSelect –5S/16S/23S, gene expression results are highly reproducible between 1 µg and 100 ng (Log2, FPKM >0).
9
QIAseq FastSelect –5S/16S/23S vs. other methods: rRNA removal
Total RNA was isolated from Gut Microbiome Whole cell Mix (ATCC) using the RNeasy PowerMicrobiome Kit (QIAGEN). Stranded transcriptome libraries were then prepared from 1 µg aliquots of the RNA using the QIAseq Stranded Total RNA Lib Kit. For rRNA depletion, three methods were used: Supplier TF – method ME and Supplier TF – method RM, which used a hybrid capture workflow, and Supplier QIAGEN – method QIAseq FastSelect –5S/16S/23S. For QIAseq FastSelect –5S/16S/23S, the QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps were completed (starting with 1st strand synthesis). For rRNA depletion using method ME and method RM, the workflows were followed according to the manufacture’s recommendations. Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

QIAseq FastSelect –5S/16S/23S removed over 90% of the 5s/16s/23s rRNA, while methods ME and RM removed less than 10% of the same rRNAs.
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QIAseq FastSelect –5S/16S/23S vs. other methods: sequencing reads
Total RNA was isolated from Gut Microbiome Whole cell Mix (ATCC) using the RNeasy PowerMicrobiome Kit (QIAGEN). Stranded transcriptome libraries were then prepared from 1 µg aliquots of the RNA using the QIAseq Stranded Total RNA Lib Kit. For rRNA depletion, three methods were used: Supplier TF – method ME and Supplier TF – method RM, which used a hybrid capture workflow, and Supplier QIAGEN – method QIAseq FastSelect –5S/16S/23S. For QIAseq FastSelect –5S/16S/23S, the QIAseq FastSelect reagent was added to the RNA sample, followed by fragmentation (89°C for 8 min) and stepwise cooling from 75°C to 25°C over 14 minutes. Following a bead cleanup, the remaining library prep steps were completed (starting with 1st strand synthesis). For rRNA depletion using method ME and method RM, the workflows were followed according to the manufacture’s recommendations. Sequencing was performed on a NextSeq 550, and data analysis was performed using CLC Genomics Workbench.

By efficiently removing 5S/16S/23S rRNA, the QIAseq FastSelect –5S/16S/23S method frees up substantial read budget, with more than 90% of the sequencing reads being available for detection of other RNAs and gene expression analysis.
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Bacterial rRNA depletion: alternative products vs. QIAseq Fast Select
QIAseq FastSelect –5S/16S/23S provides a simple, streamlined workflow vs. alternative products.
Performance

In accordance with QIAGEN’s ISO-certified Quality Management System, each lot of the QIAseq FastSelect –5S/16S/23S Kit is tested against predetermined specifications to ensure consistent product quality.

QIAseq FastSelect –5S/16S/23S Kits provide robust rRNA removal from a variety of sample types.
See figures:
QIAseq FastSelect –5S/16S/23S: robust rRNA removal from gut community sample
QIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community (28 strains)
QIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community: mapping analysis (28 strains)

QIAseq FastSelect –5S/16S/23S Kits deliver highly efficient and reproducible rRNA removal.
See figures:
QIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiome
QIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiome: gene expression results
Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S
Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression results
Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression results (100 ng, 1 µg)

QIAseq FastSelect –5S/16S/23S Kits demonstrated highly efficient removal of rRNA and superior performance compared to alternative methods.
See figures:
QIAseq FastSelect –5S/16S/23S vs. other methods: rRNA removal
QIAseq FastSelect –5S/16S/23S vs. other methods: sequencing reads
QIAseq FastSelect –5S/16S/23S vs. other methods: total reads mapped to rRNA


QIAseq FastSelect –5S/16S/23S Kits are compatible with a wide range of RNA-seq library kits.
See figures:
QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB
QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene detection
QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene expression

Principle

QIAseq FastSelect –5S/16S/23S is a pan-bacterial ribosomal RNA (rRNA) removal kit designed to remove 5S, 16S and 23S rRNA from complex bacterial community samples.  Our comprehensive rRNA removal reagent has been designed using 16S (nearly 600,000 entries), 23S (nearly 170,000 entries) and 5S rRNA sequences (over 7200 entries). In-silico modeling predicts >95% rRNA removal of all known 5S, 16S and 23S rRNA sequences.

QIAseq FastSelect –5S/16S/23S can accommodate RNA amounts ranging from as little as 20 ng to 1 µg, with consistently high performance, and is compatible with low-quality, highly fragmented or high-quality full-length RNA. Since QIAseq FastSelect –5S/16S/23S does not use enzymatic digestion or hybrid-capture procedures, the fast, simple workflow results in reliable rRNA removal and high reproducibility in downstream applications.

Procedure

Most RNA removal or depletion strategies associated with RNA-seq library construction are sample pre-treatments involving hybrid-capture or enzymatic removal of unwanted RNA. Our unique QIAseq FastSelect procedure is significantly faster and is compatible with most RNA-seq library kits.  QIAGEN FastSelect –5S/16S/23S has been tested with QIAGEN, Illumina and NEB stranded RNA-seq library kits.

QIAseq FastSelect –5S/16S/23S seamlessly integrates with your existing RNA-seq library preparation, providing RNA removal in a single, 14-minute step, which is followed by bead cleanup (figure QIAseq FastSelect –5S/16S/23S workflow). Prior to RNA heat fragmentation (which is optional and dependent upon the library preparation kit and sample type), QIAseq FastSelect –5S/16S/23S removal reagent is directly combined with total RNA and fragmentation buffer. After optional fragmentation, the reaction temperature is stepwise cooled from 75°C to 25°C over 14 minutes, followed by a bead cleanup. The RNA is then ready for reverse transcription.  This is dramatically faster than other RNA depletion methods, which require more than 25 steps and approximately 2 hours to complete (figure Bacterial rRNA depletion: alternative products vs. QIAseq Fast Select –5S/16S/23S).

Applications

QIAseq FastSelect –5S/16S/23S delivers rapid, reliable rRNA removal for bacterial isolates, as well as complex bacterial communities.

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