DNeasy PowerSoil Pro Kit

For the isolation of microbial genomic DNA from all soil types

Features

  • Efficient lysis of bacteria and fungi in all soil types
  • Up to 8-fold higher DNA yields compared to alternative methods
  • Recovery of inhibitor-free DNA for direct use in NGS applications
  • Unbiased results accurately represent sample alpha diversity
  • Automation on the QIAcube    
DNeasy PowerSoil Pro Kit (250)

Cat. No. / ID: 47016

For the isolation of microbial genomic DNA from all soil types.
$1,666.00
Preparations
250
50
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The DNeasy PowerSoil Pro Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Extracting microbial DNA from soil samples can be challenging. QIAGEN’s new DNeasy PowerSoil Pro Kit is even more effective than our original PowerSoil technology at isolating high yields of pure microbial DNA from all soil types, including compost, clay and top soil. The kit features a novel bead tube and optimized chemistry for more efficient lysis of soil bacteria and fungi. The kit also contains streamlined Inhibitor Removal Technology (IRT) to eliminate the challenging inhibitors commonly found in soil and environmental samples in even less time. Sequencing results reveal higher alpha diversity as measured by observed operational taxonomic units (OTUs) compared to other tested methods. Extraction of DNA using the DNeasy PowerSoil Pro Kit can be automated on the QIAcube Connect
Want to try this solution for the first time? Request a quote for a trial kit.

Performance

Significantly increased DNA yields from challenging samples

The DNeasy PowerSoil Pro Kit includes a novel bead tube and improved lysis chemistry, which enables isolation of up to 8-fold higher yields of DNA compared to the first generation DNeasy PowerSoil Kit and those of competitors in all soil types tested (See: ' Isolate more high-quality genomic DNA.'). Both the DNeasy PowerSoil Kit and the new DNeasy PowerSoil Pro Kit isolated high-quality DNA from challenging soil types (See: ' Improved, high-quality DNA yields with the new DNeasy PowerSoil Pro Kit.').

Unbiased identification of total diversity and community representation in soil samples


DNA isolated using the DNeasy PowerSoil Pro Kit identified more bacteria (OTUs) in soil samples when compared with the DNeasy PowerSoil Kit and those of two competitors (See: ' Increased bacterial OTUs with the new DNeasy PowerSoil Pro Kit.').
See figures

Principle

Inhibitor Removal Technology increases purity of isolated DNA

The DNeasy PowerSoil Pro Kit features a streamlined Inhibitor Removal Technology (IRT) to decrease sample processing time. In a comparison of methods for DNA isolation from soil, the DNeasy PowerSoil Pro Kit was the only method showing 260/280 ratios near 1.8 for all soil types, as well as the highest 260/230 ratios, indicating the absence of inhibitors. Samples isolated using the kit also showed no inhibition in PCR, in contrast to competitor methods (See: ' Increased purity and less variability in DNA extracted with the DNeasy PowerSoil Pro Kit.').
See figures

Procedure

The soil or stool samples are lysed via chemical and mechanical homogenization. Lysis buffer is added to a mixed zirconium bead tube containing the sample. Bead beating can be carried out using a standard benchtop vortex with bead tube adapter or the high-powered PowerLyser. Crude lysate is then subjected to inhibitor removal for cleanup. Following the cleanup, purified lysate is mixed with an equal volume of DNA binding solution and passed through a silica spin filter membrane. The membrane is washed with a two-step washing regime. Silica-bound DNA is then eluted using a 10 mM Tris elution buffer. Relevant downstream applications for the isolated DNA include PCR, NGS and enzymatic digestion assays.

Supporting data and figures

Resources

Webinars (3)
The work presented here thus offers an original assessment of the dynamics at play in the tree phyllosphere.
This webinar will focus on the acquisition and development of the preterm gut microbiome from birth and following discharge from intensive care.
This webinar will focus on the automation of QIAGEN’s new line of DNA and RNA sample prep kits for the microbiome.
Quick-Start Protocols (1)
Kit Handbooks (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699