Dispensing pressures and pulse time settings must be checked and, if necessary, updated every time a cartridge from a new lot number is used.
Managing instrument parameters in PyroMark Q96 ID Software
- Click “Instrument” and then “Parameters” to reveal the list of installed instrument parameter files.
- Right-click an existing instrument parameter file from the list and click “Duplicate”.
- Enter a new file name (e.g., “Instrument parameters Code 0005”).
- In the following table, find the dispensing pressures and pulse time settings that correspond to the code number printed on the label of the cartridge to be used.
- Enter the dispensing pressures and pulse times in the corresponding fields of the “Instrument Parameters” dialog box.
- Save the new instrument parameters file.
- When creating a new Run Setup, select this new instrument parameters file (e.g., “Instrument parameters Code 0005”) from the list of instrument parameter files.
Instrument methods for various lots of PyroMark Q96 Cartridges (cat. no. 979004) for the PyroMark Q96 ID instrument
Dispensing pressures (mbar) | Pulse times (ms) | ||||
---|---|---|---|---|---|
Code | Nucleotides | Reagents | Enzyme mix | Substrate mix | Nucleotides |
0001 | 650 | 400 | 90 | 88 | 8.5 |
0002 | 650 | 400 | 97 | 88 | 8.5 |
0003 | 650 | 400 | 109 | 99 | 8.5 |
0004 | 650 | 400 | 126 | 115 | 8.5 |
0005 | 650 | 400 | 126 | 115 | 9.0 |
0006 | 650 | 400 | 112 | 110 | 9.4 |
0007 | 650 | 400 | 124 | 114 | 8.7 |
0008 | 650 | 400 | 124 | 114 | 9.0 |
0009 | 650 | 400 | 124 | 114 | 8.0 |
0010 | 650 | 400 | 114 | 98 | 9.0 |
0011 | 650 | 400 | 114 | 98 | 8.1 |
0012 | 650 | 400 | 124 | 114 | 9.2 |
0013 | 650 | 400 | 120 | 110 | 9.2 |
0014 | 650 | 400 | 124 | 112 | 9.2 |
0015 | 650 | 400 | 120 | 110 | 9.2 |
0016 | 650 | 400 | 116 | 106 | 9.2 |
Managing instrument parameters in PyroMark CpG Software
- Access the available downloadable instrument method files below or under "Resources" on the PyroMark Q96 ID product page.
- Click the instrument parameter file that corresponds to the code printed on the label of the cartridge to be used.
- Unzip the downloaded file and save the instrument parameter file to the operator computer:
- If using PyroMark CpG SW Version 1.0.9 or lower, save the file to C:\Program Files\Biotage\Pyro Q-CpG 1.0.X\Instrument Parameters (where "X" represents the installed version of PyroMark CpG Software)
- If using PyroMark CpG SW Version 1.0.11 or higher, save the file to
C:\Program Files\Qiagen\PyroMark CpG Software 1.0.11.14\Instrument parameters
The downloaded file will now appear in the list of available instrument parameter files and can be selected when creating a new Run Setup.