HRM Technology - Key Citations

Key publications demonstrating the successful use of HRM technology

HRM technology has been successfully utilized in diverse applications as illustrated by the citations listed below.
Application Title Citation
Microbiology The use of high-resolution melting
analysis for genotyping Symbiodinium
strains: a sensitive and fast approach
 Granados-Cifuentes C. and
Rodriguez-Lanetty M. (2011) Mol.
Ecol. Resour. 11.
Microbiology
 A rapid real-time PCR/DNA
resolution melting method to
identify Prototheca species
 Ricchi. M., Cammi. G., Garbarino,
C.A., Buzzini, P., Belletti, G.L., and
Arrigoni. N. (2011) J. Appl. Microbiol.
110, 27.
Microbiology
 Detection of vancomycin-resistant
enterococci using multiplex real-time
PCR assay and melting curve analysis
 Cha, C.H., An, H.K., and Kim, J.U.
(2010) Korean J Lab Med. 30, 138.
Microbiology
 Rapid identification of maternal lineages
in common carp (Cyprinus carpio L.)
using real-time PCR and high resolution
melt-curve analysis
 Haynes, G.D., Gongoraa, J., Nicholas,
F.W., and Zenger, K.R. (2009) Aquaculture,
287, 59.
Microbiology
 Genotyping of Chlamydophila psittaci
by real-time PCR and high-resolution
melt analysis
 Mitchell, S.L. et. al. (2009) J. Clin.
Microbiol. 47, 175.
Microbiology
 Classification of fowl adenovirus
serotypes by use of high-resolution
melting-curve analysis of the hexon
gene region
 Steer, P.A., Kirkpatrick, N.C., O'Rourke,
D., and Noormohammadi,
A.H. (2009) J. Clin. Microbiol. 47, 311.
Methylation
analysis/epigenetics		 Quantitative evaluation of DNA
methylation by optimization of
a differential-high resolution melt
analysis protocol
 Malentacchi, F., Forni, G., Vinci, S.,
and Orlando, C. (2009) Nucleic Acids
Res., 37, 86.
Methylation
analysis/epigenetics
 Detection of MGMT promoter
methylation in normal individuals
is strongly associated with the T allele
of the rs16906252 MGMT promoter
single nucleotide polymorphism
 Candiloro, I.L.M. and Dobrovic, A.
(2009) Cancer Prevention Research,
2, 862.
Methylation
analysis/epigenetics
 Methylation-sensitive high-resolution
melting
 Wojdacz, T.K., Dobrovic, A., and
Hansen, L.L. (2008) Nat Protoc.
2, 1903.
SNP genotyping Nucleoside and nucleotide analogs
select in culture for different patterns
of drug resistance in Human
Immunodeficiency Virus Types 1 and 2
 Ntemgwa, M.L. et al. (2009)
Antimicrob. Agents Chemother.
53, 708.
SNP genotyping
 Implication of sex and SORL1 variants
in Italian patients with Alzheimer disease
 Cellini, E. et. al. (2009) Arch Neurol.
66, 1260.
Mutation analysis
 ScreenClust: Advanced statistical
software for supervised and unsupervised
high resolution melting (HRM) analysis
 Reja, V. et.al. (2010). Methods
(San Diego, Calif.). 4, 10.
Mutation analysis
 Limited copy number-high resolution
melting (LCN-HRM) enables the
detection and identification by sequencing
of low level mutations in cancer biopsies
 Do, H. and Dobrovic, A.
(2009) Mol. Cancer. 8, 82.
Mutation analysis
 An investigation of the C77G
and C772T variations within
the human protein tyrosine
phosphatase receptor type C gene
for association with multiple
sclerosis in an Australian population
 Szvetko, A.L. et al.
(2009) Brain Research,
1255, 148.
Mutation analysis
 A high-throughput candidate gene
mutation screen in lymphoproliferative
and myeloproliferative neoplasias
 Kreil, S., Hochhaus, A., Cross, N.C.P.,
and Chase, A. (2009) Leukemia Research,
33, 168.
Mutation analysis
 Frequent CBL mutations associated
with 11q acquired uniparental disomy
in myeloproliferative neoplasms
 Grand, F.H. et al. (2009) Blood,
113, 6182
Mutation analysis
 Plasmid-borne blaSHV genes in
Klebsiella pneumoniae are associated
with strong promoters
 Turner, M.S., Andersson, P., Bell, J.M.,
Turnidge, J.D., Harris, T., and Giffard
P.M. (2009) J. Antimicrob. Chemother.
64, 960.
Mutation analysis
 Development of a high-resolution
melting method for mutation
detection in familial
hypercholesterolaemia patients
 Whittall, R.A. et al. (2009)
Ann Clin Biochem, 10.
Mutation analysis
 Analysis of blaSHV codon 238
and 240 allele mixtures using
SYBR Green high-resolution
melting analysis
 Andersson, P., Harris, T., Tong,
S.T.Y.C., and Giffard, P.M. (2009)
Antimicrob. Agents Chemother.
53, 2679.
Mutation analysis High resolution melting analysis
for rapid and sensitive EGFR
and KRAS mutation detection
in formalin fixed paraffin
embedded biopsies
 Do, H., Krypuy, M., Mitchell,
P.L., Fox, S.B., and Dobrovic,
A. (2008) BMC Cancer. 21, 142.