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Introduction

WGA applications

DNA amplified using MDA-based WGA is suitable for use in:

  • Next-generation sequencing
  • Genotyping using microarrays
  • Comparative genome hybridization studies (CGH)
  • Single nucleotide polymorphism (SNP) genotyping
  • Sanger sequencing
  • STR/microsatellite analysis
  • Haplotyping
Next Multiple displacement amplification WGA
Previous Whole transcriptome amplification WTA
    • PCR based WGA
    • Whole transcriptome amplification WTA
      • WTA techniques
    • WGA/WTA applications
    • Multiple displacement amplification WGA
      • Unbiased amplification
      • The importance of DNA denaturation in WGA
      • WGA from single cells
      • Working with fragmented DNA
      • Working with fixed tissue samples: DNA from FFPE tissue
    • Critical factors that influence downstream PCR-based analysis following MDA
    • References