Looking to the future of missing persons analysis with NGS
Under the watchful eye of Thomas Parsons, the International Commission on Missing Persons has become the global reference in forensic human identification. As the ICMP establishes its new headquarters in The Hague, it presents the opportunity to raise its expertise to the next level.
A definitive center of excellence
Responsible for creating this facility will be Thomas Parsons, the ICMP’s Director of Science and Technology. Quiet-spoken and precise, Parsons leads the ICMP’s team of experts in forensic anthropology, forensic archeology, DNA profiling, genetic kinship matching and informatics. Although his new laboratory space is still at a very early stage, his vision for it, and what it can deliver, is clear. “This is the opportunity to establish a definitive center of excellence for DNA-based human identification,” he says.
At the same time, he also wants the facilities in The Hague to benefit from the mindset that helped the ICMP achieve so much in former Yugoslavia. In Sarajevo, Parsons explained, his team pursued a single-minded approach to problem solving. It was this mindset that drove many of the advances that DNA laboratories around the world now benefit from. “We continuously updated our techniques, with unrelenting focus. We regularly asked ourselves ’What is currently holding us back? How do we address it?’ Once we had solved one issue, we would repeat the process to tackle the next.”
Answering fundamental questions
However, Parsons is quick to stress that although the science is important, ultimately it’s about the difference that it makes to families and individuals. He and his team are hugely motivated by the consequences of their work for families of missing persons. “Our efforts bring a degree of resolution for these anguished people. We may not cure anything, but in many instances we can answer some of their most fundamental questions and resolve some of the causes of their ongoing trauma.” But equally importantly, Parsons appreciates what ICMP’s work contributes to justice. “Where perpetrators believed they would be able to remove people from the human experience, we provide irrefutable forensic evidence of identity. This means that perpetrators can be held accountable.”With the new laboratory in The Hague, Parsons and his team hope to build on these capabilities. He is eager to leverage the potential of next-generation sequencing (NGS), which he believes will address many of the existing challenges in delivering large-scale missing persons identification. Parsons explains: “There are three problems that currently hold us back; the unit cost per test; the difficulty of working with highly degraded samples and the amount of genetic information needed to confirm identity via distant family members. Advancements in next-generation sequencing clearly offer the potential to address these issues.
Turning the promise of NGS into reality
To turn the promise of NGS into reality, Parsons and his team are focusing on developing novel Single Nucleotide Polymorphism (SNP)-based testing methods. These will identify singular mutations in the human genome and provide greater power for establishing kinship when used in large numbers. The objective is to develop highly homogeneous and cost-effective laboratory mechanisms that can be applied irrespective of the level of sample degradation. Ultimately, he hopes to make this feasible at around one tenth of the current cost by pooling samples using molecular barcoding mechanisms.
Many in the field of forensic genetics foresee that an SNP-based approach may best capitalize on the strengths of NGS, and Parsons believes that with carefully directed effort it may even replace STR analysis as the standard approach in current human ID applications. Certainly, he views NGS as the way forward, not only for the ICMP but also for the entire forensic community, allowing it to address some of the major existing challenges in missing persons investigations.
At the forefront of human identity testing
The new laboratories in The Hague mark another step in the long-standing collaboration between the ICMP and QIAGEN. The relationship dates back to the ICMP’s initial work in Bosnia and Herzegovina, where QIAGEN helped the organization implement highly efficient approaches for isolating DNA from bone. The next phase will see QIAGEN provide support in developing SNP-based assays. The new ICMP laboratory will also take delivery of the QIAGEN GeneReader NGS System, the first fully integrated NGS workflow solution.
For QIAGEN, the collaboration with the ICMP is a unique opportunity. “The ICMP is at the forefront of human identity testing around the world, pioneering methods and principles for analyzing challenging samples,” says Keith Elliott, QIAGEN’s Senior Global Product Manager in Applied Testing. “What makes the ICMP so special is their commitment to leveraging science to uphold basic human rights and justice for missing persons and their families. Working with Thomas Parsons and his team in developing Sample to Insight next-generation sequencing is a huge privilege for QIAGEN.”
Parsons believes that within the next three to five years capabilities will advance substantially from where they are today. He is dedicated to maximizing the impact of these technological advances on the ICMP’s mission. “This is a worldwide crisis on many levels; economic displacement, mass disaster, terrorism, post-conflict, ongoing conflict, all of these. We have already demonstrated some of the possibilities; now we want to expand those as broadly as we can. Our cooperation with QIAGEN is a major benefit for this objective.”
About the ICMP
Founded in 1996, since 2001 the International Commission on Missing Persons (ICMP) has provided the benchmark for DNA identification techniques, combining forensic archeological and anthropological techniques with state-of-the-art DNA matching processes. To date, it has assisted national authorities in resolving the fate of some 70 percent of the 40,000 people who disappeared during the war in former Yugoslavia, with DNA assisted identifications of over 20,000 individuals worldwide; an unprecedented achievement. In 2015, the ICMP entered a new phase as an internationally recognized, treaty-based international organization and moved its base from Sarajevo, Bosnia and Herzegovina, to its new headquarters in The Hague in the Netherlands. There, the ICMP will sit alongside other institutions including the International Court of Justice and the International Criminal Court.