Switch easily to advanced end-point PCR products
New and improved end-point PCR solutions
In our effort to bring you the best and latest of PCR technologies, the TopTaq/HiFi/LongRange PCR product lines have been discontinued in production by October 15, 2020. The technologically-superior and thoroughly-validated successor kits offer multiple advantages over the phased-out products.
- Antibody-mediated hot-start of polymerase and exonuclease ensures superior specificity and sensitivity
- Master mix format ensures exceptional protocol speed and convenient handling
- Master Mix and Template Tracers prevent pipetting errors and track gel
- Improved room temperature stability enables automation
End-point PCR kits to be phased out and their recommended successors
|To-be-phased-out PCR kit||Cat. No.||Recommended successor kit||Cat. No.|
|TopTaq PCR and EpiTect MSP Kits||AllTaq PCR Kits|
|TopTaq DNA Polymerase (50)||200201||203123|
|TopTaq DNA Polymerase (250)||
|AllTaq PCR Core Kit (250 U)||203123|
|TopTaq DNA Polymerase (1000)||200205||203125|
|TopTaq DNA Polymerase (5000)||200207||203127|
|TopTaq Master Mix Kit (250)||200403||203144|
|EpiTect MSP Kit (100)||59305||AllTaq Master Mix Kit (500)||203144|
|HotStar HiFidelity Polymerase Kits||UCP HiFidelity PCR Kits|
|HotStar HiFidelity Polymerase Kit (100)||202602||202742|
|HotStar HiFidelity Polymerase Kit (1000)||202605||202744|
|QIAGEN LongRange Kits||UltraRun LongRange Kits|
|QIAGEN LongRange PCR Kit (20)||206401||206442|
|QIAGEN LongRange PCR Kit (100)||206402||206442|
|QIAGEN LongRange PCR Kit (250)||206403||206444|
Why you should switch to the successor kit – product highlights at a glance
FAQs – Find answers to your product-related queries
How does the performance of the successor kit compare to my currently used kit?
QIAGEN has developed the successor kits to improve both performance and handling, speed, or other features over the precursor version. Please visit the catalog page of the successor kit to learn about these features or to download the protocol.
The performance paragraph, and particularly the "image lightbox," will contain performance and comparison data versus the precursor and other commonly used PCR kits.
You can also download the respective product profiles or visit the catalog pages linked above to view performance.
How can I request a trial kit of the successor kit?
Please visit the catalog page of the successor kit to learn about these features and click the "request trial kit" link. This will automatically create a request form and submit your request. Please note: rules and legal requirements for providing trial kits might differ in your country.
How do I ensure the optimal performance of the successor kit?
As a general rule, please do follow the protocol of the successor kit. It has been optimized for the newly developed kit composition and provides various benefits like increased speed, yield and specificity. You will only benefit from these improvements when following the recommended protocol. Refrain from copy-pasting protocols from other or older kits.
If you should have any technical questions, our technical service team is happy to support you.
What is the recommended replacement for the EpiTect MSP Kit?
The EpiTect MSP Kit contains a slow-reading polymerase, that is particularly sensitive to single nucleotide mismatches at 3' end of primers. Although our AllTaq PCR kit provides a highly specific Guard-protected hot start, it is more processive than the EpiTect MSP Kit. However, it might not be similarly sensitive to single nucleotide mismatches. We, unfortunately, do not offer an enzyme anymore, that behaves identically to the EpiTect MSP Kit.
I'm using the older QIAGEN kit in a validated procedure, or a customized version, and cannot easily change my lab routine. How can I ensure a seamless transition?
Please contact your QIAGEN account manager or contact us directly if the phase-out of the kits mentioned above creates significant issues. We are very willing to support the continuous performance of your laboratory routine and are confident in finding the right solution. Please let us know what your requirements or issues are at www.qiagen.com/techsupport-web or use the chat feature.
What are my ordering conditions for the successor kit?
Terms and conditions apply as listed on the catalog pages. If your institution or account has specific agreement or requirements, please contact your QIAGEN account manager. Any exclusive agreement requires implementation via proper quoting and setup before becoming active in our ordering system.