The most advanced portfolio of real-time PCR and RT-PCR kits
The QuantiNova kit family represents our most advanced generation of real-time PCR and RT-PCR kits and perform equivalent or better than QuantiFast and Rotor-Gene kits. The QuantiNova kits will replace the QuantiFast and Rotor-Gene PCR kit product lines, both of which will be discontinued in production by June 30, 2021, and afterward, offered only until stocks last. The QuantiNova kits provide numerous advantages such as improved handling, speed and in-process safety tools for superior reliability and efficiency of your workflow.
- Built-in visual pipetting control prevents handling errors
- Internal Control RNA and Assays prevent inhibitor-induced mis-quantification
- The stringent Guard-protected hot-start of polymerase provides premium specificity
- Unique 2-phase hot-start for 1-step RT-PCR facilitates room temperature setup
- Ultrafast protocols for cDNA and PCR enables RNA to result in around 1 hour
- Reverse transcription with integrated gDNA removal prevents gDNA-induced mis-quantification
Phased-out QuantiFast/Rotor-Gene kits and their recommended successors
| Phased-out kit | Cat. No. | QuantiNova successor kits | Cat. No. |
|---|---|---|---|
| SYBR® Green PCR kits | |||
| QuantiFast | QuantiNova |
||
| QuantiFast SYBR Green PCR Kit (400) | 204054 | QuantiNova SYBR Green PCR Kit (500) | 208054 |
| QuantiFast SYBR Green PCR Kit (2000) | 204056 | QuantiNova SYBR Green PCR Kit (2500) |
208056 |
| QuantiFast SYBR Green PCR Kit (4000) |
204057 | QuantiNova SYBR Green PCR Kit (7500) |
208057 |
| QuantiFast SYBR Green RT-PCR Kit (400) |
204154 | QuantiNova SYBR Green RT-PCR Kit (500) |
208154 |
| QuantiFast SYBR Green RT-PCR Kit (2000) |
204156 | QuantiNova SYBR Green RT-PCR Kit (2500) |
208156 |
| Rotor-Gene | QuantiNova | ||
| Rotor-Gene SYBR Green PCR Kit (400) | 204074 | QuantiNova SYBR Green PCR Kit (500) | 208054 |
| Rotor-Gene SYBR Green PCR Kit (2000) | 204076 | QuantiNova SYBR Green PCR Kit (2500) | 208056 |
| Rotor-Gene SYBR Green RT-PCR Kit (400) | 204174 | QuantiNova SYBR Green RT-PCR Kit (500) | 208154 |
| Probe PCR kits | |||
| QuantiFast | QuantiNova | ||
| QuantiFast Probe PCR Kit (400) | 204254 | QuantiNova Probe PCR Kit (500) | 208254 |
| QuantiFast Probe PCR Kit (2000) | 204256 | QuantiNova Probe PCR Kit (2500) | 208256 |
| QuantiFast Probe PCR Kit (4000) |
204257 | QuantiNova Probe PCR Kit (7500) | 208257 |
| QuantiFast Probe PCR + ROX Vial Kit (400) | 204354 | QuantiNova Probe PCR Kit (500) | 208254 |
| QuantiFast Probe PCR + ROX Vial Kit (2000) |
204356 | QuantiNova Probe PCR Kit (2500) |
208256 |
| QuantiFast Probe RT-PCR Kit (400) | 204454 | QuantiNova Probe RT-PCR Kit (500) | 208354 |
| QuantiFast Probe RT-PCR Kit (2000) |
204456 | QuantiNova Probe RT-PCR Kit (2500) |
208356 |
| QuantiFast Probe RT-PCR + ROX Vial Kit (400) | 204554 | QuantiNova Probe RT-PCR Kit (500) | 208354 |
| QuantiFast Probe RT-PCR + ROX Vial Kit (2000) |
204556 | QuantiNova Probe RT-PCR Kit (2500) |
208356 |
| QuantiFast Probe RT-PCR PLUS Kit (80) | 204482 | QuantiNova Probe RT-PCR Kit (100) | 208352 |
| QuantiFast Probe RT-PCR PLUS Kit (400) |
204484 | QuantiNova Probe RT-PCR Kit (500) | 208354 |
| Rotor-Gene | QuantiNova | ||
| Rotor-Gene Probe PCR Kit (400) | 204374 | QuantiNova Probe PCR Kit (500) | 208254 |
| Rotor-Gene Probe RT-PCR Kit (400) | 204574 | QuantiNova Probe RT-PCR Kit (500) | 208354 |
| Multiplex PCR kits | |||
| QuantiFast | QuantiNova | ||
| QuantiFast Multiplex PCR Kit (80) | 204652 | QuantiNova Multiplex PCR Kit (100) | 208452 |
| QuantiFast Multiplex PCR Kit (400) | 204654 | QuantiNova Multiplex PCR Kit (500) | 208454 |
| QuantiFast Multiplex PCR Kit (2000) |
204656 | QuantiNova Multiplex PCR Kit (2500) |
208456 |
| QuantiFast Multiplex PCR +R Kit (80) | 204752 | QuantiNova Multiplex PCR Kit (100) | 208452 |
| QuantiFast Multiplex PCR +R Kit (400) |
204754 | QuantiNova Multiplex PCR Kit (500) |
208454 |
| QuantiFast Multiplex PCR +R Kit (2000) | 204756 | QuantiNova Multiplex PCR Kit (2500) | 208456 |
| QuantiFast Multiplex PCR +R Kit (4000) |
204757 | QuantiNova Multiplex PCR Kit (2500) |
208456 |
| QuantiFast Multiplex RT-PCR Kit (80) | 204852 | QuantiNova Multiplex RT-PCR Kit (100) | 208552 |
| QuantiFast Multiplex RT-PCR Kit (400) |
204854 | QuantiNova Multiplex RT-PCR Kit (500) |
208554 |
| QuantiFast Multiplex RT-PCR +R Kit (80) | 204952 | QuantiNova Multiplex RT-PCR Kit (100) | 208552 |
| QuantiFast Multiplex RT-PCR +R Kit (400) |
204954 | QuantiNova Multiplex RT-PCR Kit (500) |
208554 |
| Rotor-Gene | QuantiNova | ||
| Rotor-Gene Multiplex PCR Kit (80) | 204772 | QuantiNova Multiplex PCR Kit (100) | 208452 |
| Rotor-Gene Multiplex PCR Kit (400) | 204774 | QuantiNova Multiplex PCR Kit (500) | 208454 |
| Rotor-Gene Multiplex RT-PCR Kit (80) |
204972 | QuantiNova Multiplex RT-PCR Kit (100) |
208552 |
| Rotor-Gene Multiplex RT-PCR Kit (400) | 204974 | QuantiNova Multiplex RT-PCR Kit (500) | 208554 |
Why you should switch to the successor kit – product highlights at a glance
FAQs – Find answers to your product-related queries
How does the performance of the successor kit compare to my currently used kit?
QIAGEN has developed the successor kits to improve both performance and handling, speed, or other features over the precursor version. Please visit the catalog page of the successor kit to learn about these features or to download the protocol.
The performance paragraph, and particularly the "image lightbox," will contain performance and comparison data versus the precursor and other commonly used PCR kits.
You can also download the respective product profiles or visit the catalog pages linked above to view performance.
How can I request a trial kit of the successor kit?
Please visit the catalog page of the successor kit to learn about these features and click the "request trial kit" link. This will automatically create a request form and submit your request. Please note: rules and legal requirements for providing trial kits might differ in your country.
How do I ensure the optimal performance of the successor kit?
As a general rule, please do follow the protocol of the successor kit. It has been optimized for the newly developed kit composition and provides various benefits like increased speed, yield and specificity. You will only benefit from these improvements when following the recommended protocol. Refrain from copy-pasting protocols from other or older kits.
If you should have any technical questions, our technical service team is happy to support you.
I'm using the older QIAGEN kit in a validated procedure, or a customized version, and cannot easily change my lab routine. How can I ensure a seamless transition?
Please contact your QIAGEN account manager or contact us directly if the phase-out of the kits mentioned above creates significant issues. We are very willing to support the continuous performance of your laboratory routine and are confident in finding the right solution. Please let us know what your requirements or issues are at www.qiagen.com/techsupport-web or use the chat feature.
What are my ordering conditions for the successor kit?
Terms and conditions apply as listed on the catalog pages. If your institution or account has specific agreement or requirements, please contact your QIAGEN account manager. Any exclusive agreement requires implementation via proper quoting and setup before becoming active in our ordering system.
Does Taq polymerase of QuantiNova and QuantiFast/Rotor-Gene kits differ?
No, both kits contain the same Taq polymerase. However, the way it is kept inactive differs. The QuantiNova kits use a unique antibody-mediated hot-start mechanism whereas the QuantiFast/Rotor-Gene kits use a chemical hot-start mechanism.
How does hot-start procedure of QuantiNova kits compare to QuantiFast or Rotor-Gene kits?
In QuantiFast and Rotor-Gene kits, Taq polymerase hot-start is controlled by a chemical mechanism. While chemical inactivation provides a highly stringent hot-start control, the reactivation is relatively slow. The QuantiNova kits employ a novel and stringent antibody-mediated hot-start control that allows reactivation of Taq polymerase in 2–3 minutes. Additionally, the enzyme is protected by a QuantiNova Guard molecule ensuring extraordinary stringency of hot-start for utmost specificity and stability.
Does reverse transcriptase of QuantiNova and QuantiFast/Rotor-Gene kits differ?
Yes, they are different. The QuantiNova kit family features a newly developed, RNaseH-deficient, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) while QuantiFast/Rotor-Gene Reverse Transcriptase is RNase H+.
The QuantiNova RT kits come with further benefits. The QuantiNova RT-PCR Kits for 1-step RT-PCR feature a unique 2-phase hot-start procedure, that allows reaction setup at room temperature and prevents unwanted, premature enzyme activity during reaction setup. The QuantiNova Probe RT-PCR Kits additionally integrate genomic DNA reduction providing increased precision in mRNA quantification.
Will visual pipetting dyes of QuantiNova kits interfere with PCR results?
No, they won‘t. The tracking dyes allow visual check of manual or automated pipetting. They have been thoroughly tested, and have been in use for several years now. We have not experienced any difficulties.
Why is ROX dye provided in a separate tube in QuantiNova kits?
This offers highest flexibility if using ABI cyclers that require ROX as reference dye. A different amount of ROX is added before use to fit the requirements of “Low ROX” and “High ROX” cyclers, as clearly outlined in our handbook. For other cyclers that typically do not require ROX as reference dye, the respective channel could be used for target detection.
Why do QuantiNova RT-PCR and Reverse Transcription kits include Internal Control (IC) RNA?
IC RNA is an important safety measure to specifically monitor the presence of inhibitors. Its use is optional, and the respective SYBR Green- or probe-labelled IC assays are available as accessories in our (web) catalog. Inhibitors carried over from RNA extraction or cDNA synthesis will not necessarily lead to complete failure of the qPCR reaction, but can compromise PCR efficiency, shifting the CT or Cq values. This effect is neither visible nor can be quantified, potentially leading to significant, non-reproducible mis-quantification in real-time PCR results. Inclusion of an internal control RNA can avoid these effects and is a standard requirement in IVD real-time PCR procedures. We recommend its use also for real-time PCR in gene expression studies, as it is provided in a convenient and easy-to-use format in our QuantiNova kits.
Will I get different results or measured values when I switch from QuantiFast/Rotor-Gene kits to QuantiNova kits ?
All results should remain highly consistent and comparable between both kit families. However, single assays might behave slightly different and RFU values for QuantiNova SYBR Green PCR kits are higher than for QuantiFast/Rotor-Gene kits, to provide a more consistent and robust signal (this does not affect CT or Cq values.)
To ensure optimal results right from the start, we highly recommend to use the standard reaction setup protocol and cycling profiles outlined in the QuantiNova Quick Start Protocol and Handbook. This way you can fully benefit from the improved QuantiNova features.
Is the Rotor-Gene Q instrument affected by this reagent kit discontinuation?
No, the Rotor-Gene Q instrument is not affected. We will continue to offer it as an integral component of our assays technologies portfolio.
I’m using QuantiFast Pathogen (RT-) PCR + IC Kits (or QuantiFast Multiplex RT-PCR Kits) in validated procedures like detection of SARS-CoV-2 or other pathogens. Are these products affected by this phase-out?
No, QuantiFast Pathogen PCR + IC Kits (Cat. no. 211352, 211354), QuantiFast Pathogen RT-PCR + IC Kit (Cat. no. 211454) and Internal Control DNA/RNA (Cat. no. 211392, 211492) are not affected by this phase-out and will remain in our portfolio. The QuantiFast Multiplex RT-PCR +R Kit (2000) (Cat. no. 204956) will also remain available. However, smaller pack sizes of the QuantiFast Multiplex RT-PCR Kit will be offered only until June 30, 2021.