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Next-generation sequencing

Never miss a microbial target

Harness the power of hybrid capture technology

Sequencing microbial genomes and genes can be notoriously problematic. Viral genomes can vary immensely in morphology, composition, size and genomic organization. Additionally, low copy numbers or high viral mutation rates can reduce sensitivity and the efficacy of amplicon-based enrichment methods. The highly diverse nature of microbial genomes and genes, particularly bacterial antimicrobial resistance (AMR) genes, makes sequencing more complex. We can help you navigate these obstacles with ease. Transform targeted viral and bacterial sequencing using our high-sensitivity, probe-based enrichment panels.

Probing for microbiological answers using the right enrichment panel

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Target respiratory viruses
Viral genomes exhibit high diversity and can be composed of DNA or RNA, or both, complicating detection and sequencing. The QIAseq xHYB Respiratory Panel includes probes for whole genome enrichment, detection and characterization of 89 separate viral targets, including SARS-CoV-2, influenza viruses, rhinovirus, parainfluenza viruses, enterovirus and more.
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Target mpox virus (MPXV)

Whole viral genome sequencing is critical for surveillance of the multi-country mpox virus (MPXV) outbreak. However, coverage issues persist when sequencing viral genomes. The QIAseq xHYB MPXV Panel works with all known MPXV clades and enables targeted enrichment of the whole MPXV genome, including ITRs. Providing high coverage and uniformity, the panel is available either as a spike-in for use with any QIAseq xHYB Viral Panel or as a standalone kit solely for MPXV whole genome sequencing.

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Target sexually transmitted pathogens
Integration of a viral genome into the host-genome, which could occur in latent infection, can make standard two-primer amplicon-based enrichment less effective. The QIAseq xHYB Viral STI Panel offers high-quality targeted enrichment, detection and genotyping of HBV, HIV-1 and 19 types of high-risk HPV, including types 16 and 18.
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Target bacterial antimicrobial-resistant genes
While deep sequencing is capable of detecting diverse antimicrobial signatures, it lacks the sensitivity of targeted sequencing approaches. The QIAseq xHYB AMR Panel offers targeted enrichment, sequencing and detection of over 2700 separate AMR gene targets.
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Target adventitious agents
NGS-based detection of adventitious agents can fulfill a critical unmet need in the detection of known and novel microbes in biopharmaceutical manufacturing. The QIAseq xHYB Adventitious Agent Panel offers targeted enrichment for the detection of 132 separate viral targets, including human adenovirus, norovirus, rotavirus, influenza viruses, SARS-CoV-2, HPV, Epstein-Barr virus, HIV, Zika virus, hepatitis viruses and more.
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Discuss your project needs
Our specialists are on hand to answer all your questions and help you find solutions to your research challenges.
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Why QIAseq?

QIAseq FX DNA Library Kit – efficient enzymatic library prep in 2.5 hours

All-enzymatic, Illumina-compatible library prep
Pair QIAseq xHYB Viral and Bacterial Panels with the QIAseq FX DNA Library UDI Kit for high-quality library prep and easy multiplexing of up to 384 samples.
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Looking to perform bacterial metagenomic analyses?
Check out our QIAseq 16S/ITS Region Panels for taxonomic profiling, using all bacterial 16S rRNA gene variable regions as well as fungal ITS regions. "Phased primers" increase the quality of reads and base calling and eliminate the need for PhiX spike-in.
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Watch the webinar

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Join our next AMR research webinar on March 9
Find inspiration from our guest speakers on precision microbiome engineering and monitoring AMR by hospital wastewater analysis.
Register
Hybrid capture for microbial detection
Title: High-sensitivity, targeted NGS variant detection of viral and bacterial pathogens: A novel solution for healthcare and environmental surveillance and research
Speaker: Dylan Barbera, M.S., Global Product Manager, Microbial NGS, Genomics
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Webinar, online event

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