QIAsprint Connect was designed to adapt
We strongly recommend using a QIAGEN-validated QIAsprint protocol.
QIAGEN-validated QIAsprint protocols provide the most reliable performance and best overall results.
Where to start?
Find existing QIAGEN-validated QIAsprint Connect protocols
We have a growing collection of validated protocols for the QIAsprint Connect covering common sample types, nucleic acid targets and workflows. Before you modify or create a new protocol, first check whether a QIAGEN-validated QIAsprint Connect protocol already exists for your sample type and application.
Starting from an existing protocol significantly reduces development time and avoids unnecessary troubleshooting.
We currently offer protocols for the following sample types and applications:
- DNA isolation from leaves, standard plant material, inhibitor-rich plant samples and whole blood
- gDNA isolation from cells and tissue
- Pathogen nucleic acid isolation from serum, plasma and swabs
- RNA and miRNA isolation from cells and tissue
- Microbial DNA and RNA isolation from stool
- Plasmid DNA isolation from bacterial pellets and cultures
We are continuously developing new QIAsprint Connect protocols. Additional validated protocols will be released from time to time to enhance flexibility and expand the application range.
Some applications may require optional add-ons. For example, QIAphish Beads enable a centrifugation-free plasmid isolation workflow through efficient magnetic capture of bacterial cells. Below, you can see the recommended combinations of PrepSets and Essential Kits used in QIAGEN-validated protocols.
Recommended combinations of PrepSets and Essential Kits for QIAGEN-validated protocols
Typical reasons for protocol adaptation on QIAsprint Connect
QIAsprint Connect is an open and flexible system. While many users work successfully with validated protocols, certain situations require protocol adjustments. Only consider making modifications if:
- Your sample type is not available.
- Your input amount is far outside the validated range.
- Your sample is stored in a special reagent.
- You need to optimize the protocol for yield, purity or throughput.
Understanding why a protocol needs to be adapted is the first step toward making the right adjustment. The following sections provide practical guidance for each of these typical scenarios as well as information on how to best overcome other common challenges.
If you need to modify a protocol, proceed with the guidance shown in Adapt/develop a protocol. Please note that any protocol modifications and developments you implement are outside our validated conditions, and performance and results cannot be guaranteed.