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QIAseq miRNA Library Kit

Gel-free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next-generation sequencing

  • Gel-free miRNA sequencing library prep from as little as 1 ng of total RNA
  • Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data
  • Bead based method to remove adapter dimers and unwanted RNA species
  • Integrated Unique Molecular Indices (UMIs) enable quantification of individual miRNA molecules
  • Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Center

Optimized reaction chemistry enables robust, miRNA-specific libraries while minimizing reaction biases and eliminating adapter dimers. Unique Molecular Indices (UMIs) tag each miRNA at an early stage, eliminating PCR and sequencing bias. Free, integrated data analysis is available through GeneGlobe for both primary miRNA mapping and UMI analysis as well as secondary differential expression analysis.

Cat No./ID: 331502
QIAseq miRNA Library Kit (12)
For 12 sequencing prep reactions: 3’ ligation, 5’ ligation, reverse-transcription, cDNA cleanup, library amplification and library cleanup reagents; quality control primers
Cat No./ID: 331505
QIAseq miRNA Library Kit (96)
For 96 sequencing prep reactions: 3’ ligation, 5’ ligation, reverse-transcription, cDNA cleanup, library amplification and library cleanup reagents; quality control primers
Cat No./ID: 331565
QIAseq miRNA NGS 96 Index IL (96)

Sequencing adapters, primers and indices compatible with Illumina platforms; 96 indices for 96 samples (pre-plated in 96-well plate for ease of use)

Cat No./ID: 331582
QIAseq miRNA NGS 12 Index TF (12)
Sequencing adapters, primers and indices compatible with Thermo Fisher platforms; 12 indices for 12 samples
Cat No./ID: 331585
QIAseq miRNA NGS 48 Index TF (96)

Sequencing adapters, primers and indices compatible with Thermo Fisher platforms; two 48 indices for 96 samples

Cat No./ID: 331592
QIAseq miRNA NGS 12 Index IL (12)
Sequencing adapters, primers and indexes compatible with Illumina platforms; 12 indexes for 12 samples
Cat No./ID: 331595
QIAseq miRNA NGS 48 Index IL (96)
Sequencing adapters, primers and indexes compatible with Illumina platforms; two 48 indexes for 96 samples
The QIAseq miRNA Library Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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Quick-Start Protocols (4)
Part 2: QIAseq miRNA NGS (QMN) Bead preparation, cDNA cleanup
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Part 3a: Library amplification using HT plate indices
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Part 3b: Library amplification using tube indices
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Part 1: 3' Ligation, 5' ligation, reverse transcription
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Kit Handbooks (2)
Precision small RNA library prep for Illumina® NGS systems
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Precision small RNA library prep for Thermo Fisher Scientific® NGS systems
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FAQs (17)
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Scientific Posters (1)
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