Digital PCR workflow and products

The QIAcuity Digital PCR System is a microfluidic nanoplate-based digital PCR (dPCR) platform that enables all the necessary steps for dPCR - partitioning, thermal cycling, and imaging/data acquisition - on a single instrument.

QIAcuity advantages

pcr dpcr qiacuity workflow

How does QIAcuity work?

Just like in qPCR experiments, sample preparation includes the transfer of master mix, probes and primers to a 96- or 24-well nanoplate, followed by the addition of samples. The system integrates partitioning, thermocycling and imaging into a single fully automated instrument that takes users from the sample to result in under 2 hours. One can perform analysis on the Software Suite, providing the concentration in copies per microliter of your target sequence as well as for quality control such as positive samples or NTC. This analysis can also be extended to remote computers within the same local area network (LAN).

Step-by-step tutorial

Getting started with QIAcuity Nanoplate dPCR can be easier than you expect. Designed for beginners, this detailed tutorial video discusses experimental considerations at each of the major steps in the workflow.
Refer to the QIAcuity Guide for more information on setting up experiments for your research applications.

The QIAcuity ecosystem

While using a QIAcuity Digital PCR System is a great foundation, it takes an ecosystem of supporting accessories, kits and assays for your digital PCR reaction to truly flourish.

You use different strategies to analyze gene expression, rare mutation detection and microbial quantification. Why struggle to find the right tools to turn those strategies into actionable insights? Explore the many options available in the QIAcuity ecosystem to customize your digital PCR runs to your specific application.

Streamline your clinical PCR workflows with QIAcuityDx

QIAcuityDx is tailored for IVD applications. This fully automated system enhances diagnostic precision and operational efficiency by reducing hands-on time and ensuring accurate detection and quantification of important genetic variations. Easily develop your own assay menu* by using QIAcuityDx utility mode and IVD medical device consumables, reagents and software.

How to choose a QIAcuity Digital PCR System

Together with its nanoplates, kits and assays, each instrument delivers high performance and data quality, with flexible throughput and multiplexing. Ask the right questions to evaluate your needs.

  • What applications will you perform most frequently?
  • Which instrumentation options best enable your scale of study?
  • What are the costs for product care beyond the initial investment?

For further guidance about comprehensive product care when making your decision, download the QIAcuity Service Brochure.


Browse a growing list of articles featuring QIAcuity dPCR products.

*FDA ‘Medical Devices; Laboratory Developed Tests’ final rule, May 6, 2024 and European Union regulation requirements on ‘In-House Assays’ (Regulation (EU) 2017/746 -IVDR- Art. 5(5))

The QIAcuity dPCR System is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Therefore, the performance characteristics of the product for clinical use (i.e., diagnostic, prognostic, therapeutic or blood banking) is unknown.

The QIAcuityDx dPCR System is intended for in vitro diagnostic use, using automated multiplex quantification dPCR technology, for the purpose of providing diagnostic information concerning pathological states.

QIAcuity and QIAcuityDx dPCR instruments are sold under license from Bio-Rad Laboratories, Inc. and exclude rights for use with pediatric applications.