Since QIAGEN launched the world’s first kit for purification of plasmid DNA, technologies have been developed to make plasmid DNA preparation even more convenient and reduce endotoxin levels in line with scientists’ needs in the laboratory. A lot of well-known companies have developed kits, and the choice of solutions available has never been greater. We compared popular advanced-technology kits to see their relative performance in speed, convenience of handling, yield and endotoxin levels.