One-step Probe RT-qPCR Mixes

For reproducible and efficient first-strand cDNA synthesis and subsequent Real-Time PCR in a single tube.

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One-Step Probe RT-qPCR Mix ROX (100)

Cat. No. / ID:  AM09.1-100

100 rxns (20 μL) Mu-MLV Reverse Transcriptase and RNase Inhibitor and ROX solution are included in separate tubes. Components: 2x 1 mL One-Step Probe RT-qPCR Mix, 40 μL 50x High ROX solution, 40 μL 50x Low ROX solution, 10 μL RNase Inhibitor, 10 μL Reverse Transcriptase, 1.9 mL Nuclease-free water Storage temperature: Store all components at -20°C. It does not lose its activity after eight successive thaw cycles. Aliquoting can be applied if necessary.
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OEM Product
One-Step Probe RT-qPCR Mix ROX
One-Step Probe RT-qPCR Mix
Reactions
100
500
This product will be discontinued as of August 31, 2024 or until stocks last.
The One-step Probe RT-qPCR Mixes is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
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Features

  • Detects low copies of RNA targets from a broad range of samples
  • Exhibits consistent amplification across a wide dynamic range
  • Eliminates non-specific amplification through precisely selected anti-Taq antibody
  • Has a high efficiency in multiplex reactions
  • Detects molecular targets accurately as fast as 40 minutes with two or three steps cycling profiles

Product Details

One-Step Probe RT-qPCR Mix is a convenient reaction mixture for fast and reliable quantitative Real-Time PCR using probes, including TaqMan ®, Scorpions ® and molecular beacon probes.

It was created for reproducible and efficient first-strand cDNA synthesis and subsequent Real-Time PCR in a single tube. It is the best choice for probe-based Real-Time PCR assays, including singleplex and multiplex gene expression studies.

Performance

Assay Specification
DNase contamination None detected
RNase contamination None detected

 

Principle

Using high-affinity antibodies for hot-start polymerase ensures higher specificity by reducing the formation of primer-dimer structures. It allows for a more comprehensive dynamic range by removing competition for reaction reagents. It also leads to higher sensitivity and reproducibility.


Precisely optimized buffer components ensure optimal reverse transcriptase and hot-start polymerase activity conditions. Additionally, RNase Inhibitor protects RNA from unspecific RNases.


The One-Step Probe RT-qPCR Mix provides fast, highly specific one-step Real-Time RT-PCR results, giving consistent results across all commonly used Real-Time PCR platforms.

Procedure

Quality Control

One-Step Probe RT-qPCR Mix is extensively tested for its performance in different Real-Time PCR assays. It is free of DNase and RNase contamination.

Applications

This is used for applications such as:

  • RT-qPCR
  • Gene expression analysis and genetic profiling
  • miRNA profiling and quantification
  • Mass screening and RNA viral pathogen detection
  • Characterization of genetically modified organisms (GMO)

 

Resources

Quick-Start Protocols (1)
Safety Data Sheets (1)
Certificates of Analysis (1)