Cat. No. / ID: AM09.1-100
One-Step Probe RT-qPCR Mix is a convenient reaction mixture for fast and reliable quantitative Real-Time PCR using probes, including TaqMan ®, Scorpions ® and molecular beacon probes.
It was created for reproducible and efficient first-strand cDNA synthesis and subsequent Real-Time PCR in a single tube. It is the best choice for probe-based Real-Time PCR assays, including singleplex and multiplex gene expression studies.
Using high-affinity antibodies for hot-start polymerase ensures higher specificity by reducing the formation of primer-dimer structures. It allows for a more comprehensive dynamic range by removing competition for reaction reagents. It also leads to higher sensitivity and reproducibility.
Precisely optimized buffer components ensure optimal reverse transcriptase and hot-start polymerase activity conditions. Additionally, RNase Inhibitor protects RNA from unspecific RNases.
The One-Step Probe RT-qPCR Mix provides fast, highly specific one-step Real-Time RT-PCR results, giving consistent results across all commonly used Real-Time PCR platforms.
One-Step Probe RT-qPCR Mix is extensively tested for its performance in different Real-Time PCR assays. It is free of DNase and RNase contamination.
This is used for applications such as: