MAPK1 (a protein kinase) and IL6 (a cytokine) in human leukocyte cDNA (1 ng) were quantified in duplicate using [A] QuantiTect Primer Assays and the QuantiTect SYBR® Green PCR Kit, or [B] probe-based assays and a real-time PCR kit from Supplier AII. QuantiTect Primer Assays provided greater sensitivity than the probe-based assays, as demonstrated by the lower CT values, as well as high specificity, as indicated by the single peaks in melting curve analysis.
[A] Different amounts of leukocyte cDNA were analyzed in duplicate using the QuantiTect Primer Assay for IL8 (a chemokine) and QuantiFast SYBR® Green PCR Kit. No template control (NTC) reactions were also performed (shown by the flat plots). [B] Melting curve analysis, demonstrating high PCR specificity.
QuantiTect Primer Assays enable significant time savings over homemade methods.