PCR/qPCR/dPCR
满怀信心地进行特异性、灵敏且稳健的 PCR
在进行基因扩增时，您的实验成功与否取决于几个关键因素，其中包括通量、检测灵敏度、反应的稳健性（即使使用困难模板）以及可靠的数据均一化。您可能还需要克服与您正在使用的 PCR 方法相关的特定挑战。
无论您选择哪种方法，我们都能为终点法 PCR、qPCR 和 dPCR 提供丰富的优化解决方案。我们的试剂盒、assay、仪器和附件可帮助您在几乎任何应用种都能更有信心地扩增和检测靶标以及简化您的工作流程。
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ΔΔCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/µL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probe‑based or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
浏览 PCR/qPCR/dPCR
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找到 133 个产品
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
QIAcuity Digital PCR System
用于基于纳米板的数字 PCR 应用
QIAcuity OneStep Advanced Probe Kit
适用于在 QIAcuity 数字 PCR 仪器上进行一步法RT-PCR
QuantiTect PCR Kit
适用于使用 SYBR Green I 的 qPCR 和两步法 qRT-PCR，qPCR 和两步法 qRT-PCR，以及使用序列特异性探针的多重 qPCR 和两步法多重 qRT-PCR
QuantiTect RT-PCR Kit
用于使用 SYBR® Green I 进行一步法 qRT-PCR 以及使用序列特异性探针进行基因表达分析的一步法 qRT-PCR 和多重 qRT-PCR
QuantiNova PCR Kit
适用于使用基于 SYBR Green 的 qPCR 进行高灵敏度、特异性和超快探针法 Real-time PCR 和多重 PCR 以及获得无与伦比的结果
数字 PCR 用 miRCURY LNA miRNA PCR Assay
使用基于 QIAcuity EvaGreen 的 dPCR 进行极其灵敏和特异性的 miRNA 定量
QuantiNova RT-PCR Kit
适用于使用序列特异性探针进行高灵敏度和特异性的一步法 qRT-PCR 和多重 qRT-PCR，或使用 SYBR® Green I 进行基因表达分析的一步法 qPCR
Custom Microbial DNA qPCR Arrays
real-time PCR方法的微生物鉴定或分析
dPCR Microbial DNA Detection Assay
用于微生物靶标的数字 PCR 检测，包括细菌、真菌、寄生虫、病毒、抗生素耐药性或毒力因子基因
REPLI-G Kits
For highly uniform whole genome amplification from 10ng of genomic DNA
QIAcuity High Multiplex Probe PCR Kit
用于具有挑战性的样本和超过五个靶标的 dPCR 多重检测
FastLane Cell RT-PCR Kits
For SYBR® Green real-time one-step RT-PCR analysis and probe-based real-time one-step RT-PCR and multiplex RT-PCR analysis from cultured cells without RNA purification
QIAcuity Cell and Gene Therapy (CGT) dPCR Assays
用于在 QIAcuity数字PCR系统上以卓越的准确性、重复性和速度进行载体基因组滴定和载体拷贝数测定
QIAcuity Residual DNA Quantification and Sizing Kits
For quantification and sizing of host cell DNA with higher accuracy and precision using the QIAcuity Digital PCR System
QIAcuity OneStep Advanced EG Kit
For dye-based one-step RT-PCR on the QIAcuity digital PCR instruments
数字 PCR 用 miRCURY LNA miRNA Custom PCR Assay
使用基于 QIAcuity EvaGreen 的 dPCR 进行极其灵敏和特异性的 miRNA 定量
Q-Solution Kit
该试剂盒包括 Q-Solution 和 MgCl2，用于在更具挑战性的设置下从 PCR 反应中获得更好的结果。
QIAcuity Mycoplasma Quant Kit and Standards
For digital PCR quantification and detection of mycoplasma contamination using the QIAcuity Digital PCR System
QIAcuity UCP Probe PCR Kit
用于 QIAcuity 数字 PCR 仪器的 Ultra Clean Production 试剂盒
dPCR Copy Number Variation (CNV) Probe Assays
用于通过单重或多重 dPCR 检测进行基因座特异性拷贝数变异及改变分析
mericon Sample Preparation and Bacteria Kits
For extraction of high-quality DNA from microbial enrichment cultures and raw or processed foods
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
UCP PCR Kits
For SYBR Green-based and probe-based, real-time quantitative PCR for microbiome or quality control applications using reagents from Ultra Clean Production
QIAprep& Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAprep& Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
VeraSeq™ ULtra DNA Polymerase
Ultrathermostable uracil-literate polymerase for high-fidelity PCR
QIAcuity mericon Food Testing Kits
For digital PCR authentication of food and animal feed using QIAcuity
dPCR PanCancer Kits
For detection of multiple hallmark mutations in cancer-relevant genes using the QIAcuity digital PCR instruments
VeraSeq™ 2.0 High-Fidelity DNA Polymerase
Ultrathermostable polymerase for high-fidelity PCR
2x HiFi PCR Master Mix
A high efficiency, high-fidelity and low bias PCR master mix for NGS library amplification.
VeraSeq™ PCR Mix
Ultrathermostable mastermix for high-fidelity PCR
mericon Ingredient Authentication Assay
For real-time PCR authentication of food and animal feed ingredients
QIAcuity Nanoplates 及附件
供与 QIAcuity 数字 PCR 仪器一起使用
QIAGEN OneStep RT-PCR Kit
用于灵敏、特异性的一步法RT-PCR
QIAGEN Multiplex PCR Kit
用于高特异性和灵敏度的多重PCR，无需优化
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