Explore enzyme solutions across the NGS library preparation workflow
Next-generation sequencing (NGS) workflows are evolving beyond standardized kits. Now you can power your NGS workflows with scalable enzyme solutions for customizable NGS sequencing applications. Advanced users and developers need flexibility, cost control and reliable performance to manage increased throughput and diversified applications. Our comprehensive portfolio of enzymes, proteins and optimized mixes supports customizable NGS library preparation for research and clinical applications as well as commercial assay development, OEM partnerships and scalable manufacturing.
Browse the interactive workflow to discover enzymes, modules and kits for each step of the NGS library preparation workflow.
Fresh tissue, FFPE tissue
or cell samples
or cell samples
DNA/RNA purification
Library preparation
Sequencing
Data analysis
Pre-library amplification & rRNA removal
cDNA Synthesis
Fragmentation
End Repair
A-Tailing
Ligation
Library Amplification
Featured enzyme solutions by workflow step
Select the right QIAseq kit for your sample and application
Pre-library amplification & rRNA removal
cDNA Synthesis
Fragmentation
End Repair
A-Tailing
Ligation
Library Amplification
Customizable next-generation sequencing: Performance, flexibility and scalability
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NGS enzyme product catalog
NGS library preparation for DNA sequencing workflows
Our NGS enzymes and mixes support optimized enzymatic fragmentation, end repair, A-tailing, ligation and amplification across DNA workflows for NGS library preparation.
| Step | Product name | Cat. number | Description |
|---|---|---|---|
| Whole Genome Amplification (WGA) |
phi29 DNA Polymerase high or low concentration |
P7020-HC-L P7020-LC-L |
A highly processive DNA polymerase with a powerful strand displacement activity and a high fidelity 3’→5’ proofreading exonuclease function |
| Fragmentation, End-repair, A-tailing |
5X WGS Fragmentation Mix | Y9140L | Customizable fragment sizes with unbiased fragmentation comparable to mechanical shearing. Provides fragmentation, end-repair and A-tailing in a single tube solution. Optimized for Illumina sequencing workflows and automated NGS library preparation. |
| End-repair, A-tailing | 5X ER/A-Tailing Enzyme Mix | Y9420L | A single-tube solution combining end repair and dA-tailing for fragmented DNA in NGS library preparation for Illumina sequencing platforms |
| End-repair | End-Repair Mix (High Concentration) |
Y9140-HC-L | This formulation is optimized for high DNA concentrations, such as library construction for sequencing |
| T4 DNA Polymerase | P7080L1 | Proven choice for polishing 5’ and 3’ of ends; end-repair | |
| End-repair/End polishing | T4 Polynucleotide Kinase | Y9040L | Phosphorylation of 5’-ends of DNA prior to ligation |
| A-tailing | Klenow (3’ → 5’ exo-) high or low concentration |
P7010-LC-L P7010-HC-L |
DNA polymerase best for dA/dT tailing for NGS adapter ligation |
| TaqIT DNA Polymerase | P7620L | Exo-deficient derivative of Taq DNA polymerase; more thermostable and with greater fidelity than full-length Taq enzyme | |
| Taq-B DNA Polymerase | P7250L | Taq DNA polymerase that produces robust yields with little or no optimization | |
| Ligation | WGS Ligase | L6030-W-L | WGS Ligase optimized for ligation following WGS Fragmentation or ER/A-Tailing |
| T4 DNA Ligase | L6030-HC-L | High-concentration rapid formulation ligase increases efficiency and decreases ligation times | |
| LoopLigase | L6130L | Enzyme for high-efficiency and low-bias intramolecular ligation (circularization) of ssDNA/ssRNA | |
| Taq DNA Ligase | L6060L | Thermostable ligase for joining or ligation of DNA fragments | |
| Tth DNA Ligase | EN13-250 | Thermostable NAD+-dependent dsDNA ligase | |
| T3 DNA Ligase | L6010L | ATP-dependent dsDNA ligase with increased salt tolerance | |
| T7 DNA Ligase | L6020L | For ligating cohesive ends of duplex DNA | |
| NGS library amplification | 2X HiFi PCR Master Mix | P7670L | A high efficiency, high fidelity, and low bias PCR master mix for NGS library amplification |
| VeraSeq™ ULtra DNA Polymerase | P7520L | High fidelity DNA polymerase that tolerates uracil-containing templates, nucleotides and primers | |
| QIAGEN Multiplex PCR Kit | 206145 | For highly specific and sensitive multiplex PCR without optimization requirements | |
| UltraRun LongRange PCR Kit | 206444 | For ultrafast, hot-start mediated, long-range PCR | |
| dNTP Set | 201912 | Individual high-purity dNTPs (dATP, dCTP, dGTP and dTTP) for flexibility and sensitive PCR workflows | |
| Efficient NGS library prep | Recombinant Albumin, AOF | 531001 | Animal-origin-free protein improves amplification yield by binding PCR inhibitors |
| T4 Gene 32 | Y9130L | A ssDNA-binding protein for increasing PCR amplification and DNA sequencing efficiency | |
| Exonuclease I | X8010L | Cleaves single-stranded DNA in the 3’ → 5’ direction, releasing 5' mono/di-nucleotides and leaving dsDNA and the 5' terminus intact |
NGS library preparation for RNA sequencing workflows
Our NGS enzymes and mixes support RNA sequencing and RNA-seq library preparation workflows with optimized reverse transcriptases, RNase inhibitors, ligases and amplification enzymes. High-quality cDNA synthesis is critical for reliable library preparation and downstream RNA sequencing accuracy.
| Step | Product name | Cat. number | Description |
|---|---|---|---|
| cDNA synthesis | EnzScript™ (MMLV Reverse Transcriptase RNase H-) |
P7600L | RNA-dependent DNA polymerase with template-switching activity and no detectable RNase H activity |
| RNase Inhibitor | Y9240L RT35 |
Non-competitive inhibitor of RNase A, B, C (porcine or human origin) | |
| Second strand cDNA synthesis | RNase H | Y9220L | Cleaves RNA strand of DNA:RNA hybrids |
| Ribosomal RNA depletion | Thermostable RNase H | Y9250-5000-FIN | Optimal activity above 65°C to cleave RNA strand of DNA:RNA hybrids for high-stringency reactions |
| Amplification | Uracil DNA Glycosylase | G5010L | An enzyme that creates abasic (AP) sites in Uracil-containing DNA |
| Thermolabile UNG | G5030L | Removes uracil from DNA leaving AP site | |
| DNA Polymerase I | P7050L | DNA polymerase with 5’→3’ synthesis, 5’→3’ and 3’→5’ exonuclease activities | |
| Bst X DNA Polymerase | P7390L | DNA polymerase homologous to the well-known DNA polymerase from Bacillus stearothermophilus (Bst) | |
| Ligation | T4 RNA Ligase 1 | L6050L | Single-stranded RNA ligase. Also joins single-stranded DNA molecules. |
| T4 RNA Ligase 2 | L6080L | Ligates single-stranded or double-stranded RNA molecules, as well as RNA:DNA hybrids | |
| T4 RNA Ligase 2 Truncated | L6070L | Requires a preadenylated 5’ phosphate-containing DNA or RNA to ligate to 3’ hydroxyl of RNA | |
| E. coli DNA Ligase | L6090L | Efficiently ligates DNA at nicks and cohesive termini | |
| LoopLigase | L6130L | Enzyme for high-efficiency and low-bias intramolecular ligation (circularization) of ssDNA/ssRNA |